Designed for adult learners taking Functional Skills courses (ICT L1 and L2) Inspired by my father and his references to
cell block H, this resource encourages people to think of the cells as prison cells, and the data inside them as prisoners.
As an alternative test for receptor specificity, we applied the 5 - HT3 receptor agonist CPG, which induced an increase in intracellular calcium in 5 - HT3AGFP
cells; the agonistic effect was
blocked by ODS (n = 5, t (4) = 20.55, p < 0.0001 paired Student's t test; Fig. 4D,
H).
The membrane was
blocked with 3 % BSA / PBS for 1
h at RT followed by incubation with the corresponding primary antibody in 1 % BSA / PBS - T overnight at 4 °C: pTyr (Millipore, # 05 - 321, 1:500), Tie2 (R&D, #AF313, 1:1,000), Tie2 (R&D, #AF762), pAKT (
Cell Signaling, # 4060S, 1:1,000), AKT (
Cell Signaling, # 9272S, 1:1,000), FOXO1 (
Cell Signaling, # 9454, 1:1,000), pFOXO3A (
Cell Signaling, # 9466, 1:1,000), FOXO3A (
Cell Signaling, # 12829, 1:1,000), VEGFR2 (
Cell Signaling, # 2479, 1:1,000), tubulin (Sigma, #T8203, 1:5,000).
Within the Maze, during 1975, several
H - shaped buildings, called
H -
blocks were constructed, each with four small wings of 25
cells.