Sentences with phrase «cell clones expressing»

Not exact matches

A team led by Byeong - Chun Lee of Seoul National University in South Korea created the dogs by cloning fibroblast cells that express a red fluorescent gene produced by sea anemones.

Functional glutamate receptor (GluRs) were transiently expressed in cultured mammalian cells from cloned complementary DNAs encoding GluR - A, - B, - C, or - D polypeptides.

When this clone was inserted into a mammalian expression vector and transfected into COS - 7 cells, a protein that specifically phosphorylated the agonist - occupied form of the beta 2 - adrenergic receptor and phosphorylated, much more weakly, the light - bleached form of rhodopsin was expressed.

The parent TZM - bl cell line is an engineered HeLa cell clone that expresses human CD4, CCR5 and CXCR4 and contains HIV - 1 Tat -

The parent TZM - bl cell line is an engineered HeLa cell clone that expresses human CD4, CCR5 and CXCR4 and contains HIV - 1 T

The parent TZM - bl cell line is an engineered HeLa cell clone that expresses human CD4, CCR5 and CXCR4 and contains HIV - 1 Ta

The mutant allele was expressed in d42m1 tumor cells and regressor clones, but absent from all escape tumors and regressor clones.

Positive clones were then differentiated using defined serum - free conditions outlined in Ng, et al [7], which overall gave rise to a CD45 + CD56 + CD117 − CD94 + population of cytotoxic NK cells [8] expressing the CD4 construct and other surface markers in a similar manner to peripheral blood NK cells.

Five beagles were created by cloning fibroblast cells that express a red florescent gene produced by sea anemones.

Following electroporation into R26 BirA cells, a cell line that expresses bacterial BirA ligase from the ROSA26 locus, hygromycin resistant clones (200 µg / ml) were expanded for Southern analysis to identify correct targeting events.

However, if it is known which unique antigen is expressed by a tumour cell type, it should be possible to clone a T - cell receptor that has the capacity to bind to that antigen.

The DT40 cell clone used expresses an inducible form of the Cre recombinase (a MerCreMer fusion protein) in order to excise the floxed AID and thereby stop mutational activity if desired.

Tags for this Online Resume: Ecl (Control Lang), Purchasing, Software, ARIA, Injections, Intelligent Call Management, Research, Transcription, Genotyping, Western blot, viral vectors, DNA cloning, RNA extraction, RNA syntehesis, confocal microscopy, cell culture, transfection, transduction, western blot, PCR, RT - qPCR, Next Generation DNA sequencing, ion torrent, gromacs, ICM, Schrodinger Tools, NCBI software, DIVA, FlowJaw, Flow Cytometry, Cell Sorting, Aria II, Aria III, XCalibur, TaqMan, electrophoresis, Sanger DNA sequencing, FCS Express, cell patterning, ELISA, immunoassay, antibody, HPLC, his - tag, flag - tag, flash - tag, chromatography, stem cells, DNA extraction, CRISPR, cas9, plasmid, lentivirus, CAR - technology, MLV, Chimeric Antigen Receptors, Jurkat, U87, HeLa ccell culture, transfection, transduction, western blot, PCR, RT - qPCR, Next Generation DNA sequencing, ion torrent, gromacs, ICM, Schrodinger Tools, NCBI software, DIVA, FlowJaw, Flow Cytometry, Cell Sorting, Aria II, Aria III, XCalibur, TaqMan, electrophoresis, Sanger DNA sequencing, FCS Express, cell patterning, ELISA, immunoassay, antibody, HPLC, his - tag, flag - tag, flash - tag, chromatography, stem cells, DNA extraction, CRISPR, cas9, plasmid, lentivirus, CAR - technology, MLV, Chimeric Antigen Receptors, Jurkat, U87, HeLa cCell Sorting, Aria II, Aria III, XCalibur, TaqMan, electrophoresis, Sanger DNA sequencing, FCS Express, cell patterning, ELISA, immunoassay, antibody, HPLC, his - tag, flag - tag, flash - tag, chromatography, stem cells, DNA extraction, CRISPR, cas9, plasmid, lentivirus, CAR - technology, MLV, Chimeric Antigen Receptors, Jurkat, U87, HeLa ccell patterning, ELISA, immunoassay, antibody, HPLC, his - tag, flag - tag, flash - tag, chromatography, stem cells, DNA extraction, CRISPR, cas9, plasmid, lentivirus, CAR - technology, MLV, Chimeric Antigen Receptors, Jurkat, U87, HeLa cells