Sentences with phrase «cell cloning experiment»

Typical success rates for the generation of clonal colonies from a single - cell cloning experiment range from 1 — 5 %.

Not exact matches

The Dolly experiment [which yielded the first cloned adult mammal, Dolly the sheep, in 1996] prompted people to find ways of taking specialized cells and transforming them into pluripotent, undifferentiated stem cells.

Further experiments went on to show that the actual level of sexual - stage parasites produced by each parasite clone matched the proportion of individual cells specifically producing the AP2 - G protein.

«Then we cultured the cells in the presence of various drugs and screened for spontaneous drug - resistant mutants to isolate clones useful for symbiosis experiments.»

These cloning experiments (known as somatic cell nuclear transfer), in addition to being unambiguously nonpresidential, require a rare and precious starting material: healthy human egg cells.

They view this as a test run for creating human embryonic stem cells in the same way (and according to the team, South Korean biologist Hwang Woo Suk seems to have accidentally accomplished this feat while executing his famously fraudulent human cloning experiment).

If the cells can be fertilized and develop into viable embryos, and if human ES cells turn out to have similar powers, such cells could allow researchers to get around some of the expense and ethical questions that arise from using donated eggs for therapeutic cloning experiments.

In their initial experiments, Hwang and his colleagues used cells from a single donor to try to create embryonic clones; they transferred nuclei from ovarian cells back into the donors» own oocytes.

Scientists have experimented with animal cloning, but have never been able to stimulate a specialized (differentiated) cell to produce a new organism directly.

While Gurdon's tadpoles did not survive to grow into adult frogs, his experiment showed that the process of specialization in animal cells was reversible, and his technique of nuclear transfer paved the way for later cloning successes.

The experiment and others like it inspired efforts to grow new organisms from the genetic material of an adult cell, and put the word «clone» into the popular vocabulary.

To determine if T - cell - mediated immunoselection was responsible for the outgrowth of the escape tumors, Matsushita et al performed a fascinating experiment using a mixture of cells from two d42m1 clones:

The Korean scientists say they do not plan to conduct further cloning experiments, but will instead focus on developing therapies from the stem cells they've produced.

The experiments show that cooperation between different cell clones can be essential for tumor progression.

Importantly, when the three holoclone - derived tumors were harvested and PC3 - GFP cells were purified and used in secondary tumor transplantation experiments, 1,000 cells of each clone regenerated tumors in 70 % to 100 % of the injections (Table 1; Fig. 3B).

The investigator is responsible for demonstrating a reliable scheme for identifying targeted ES cell clones by Southern analysis with 5» and 3» probes by providing results from pilot experiments for this purpose.

We have proposed simultaneous experiments to optimize the process of cloning Black - footed Ferrets using domestic ferrets as surrogate egg cell donors and mothers, thus avoiding using endangered Black - footed Ferrets for experimental procedures.

The experiment that led to the cloning of Dolly the sheep in 1997 was different: It used a cloning technique called somatic cell nuclear transfer and resulted in an animal that was a genetic twin — although delayed in time — of an adult sheep.

However, it is practically difficult to perform the rescue experiments in the organoid culture system because the lentivirus transfection is required for the gene transduction into the primary stem / progenitor cells and the APC gene (> 8.5 k base pairs) is too large to be cloned into the lentivirus.

Tags for this Online Resume: Lentiviral Retroviral shRNA RNA knockdown screens, Flow Cytometry, RTPCR, Immunopreciptations, Reporter assays, Cell based Assays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, Cell based Assays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, FACS