Sentences with phrase «cell colonies»

In the photo, the embryonic stem cell colonies are the rounded, dense masses of cells.

A single co-transfection of pooled Cas9 / gRNAs enabled isolation of cell colonies carrying simultaneous disruption of four genes with high efficiency.

MSTN / PrP double mutations in cell colony make the target locus resistant to Van91I and Bsu36I treatment (arrowhead).

When they instead boosted levels of CRIPTO, the stem cell colony grew as new stem cells were produced more often than differentiated cells.

Even though different cell types were used as the initial starting materials, and they were made to produce different sets of proteins, both groups were able to identify and isolate hESC - like cell colonies only 20 to 30 days after transduction.

MSTN mutations in cell colony make the target locus resistant to Van91I treatment (arrowhead).

Furthermore, due to high efficiency of CRISPR / Cas9 system, cell colonies carrying multiple gene mutations can be isolated by limiting dilution and is free of selection maker, which avoids further removal of selection marker by recloning or intercrossing [35].

Moreover, while antibody staining and microscopy of ES cell colonies grown in 2i showed uniform morphology, no detectable Gata6 expression and reduced Nanog heterogeneity, Venus positive cells were visible within these colonies and this Venus positive expression was rarely found within cells expressing high levels of Nanog (Figure 6D).

Overall stem cell colony formation efficiency following single cell isolation and flow cytometry was still quite low (∼ 0.5 %) despite the use of the Rho - associated kinase inhibitor but consistent results were obtained.

The stem cell community has repeatedly called for the restrictions to be lifted, charging that the designated cell colonies have been compromised or corrupted and that failure to ease the ban is hobbling U.S. efforts to discover new life - saving treatments.

«Only then can we understand in detail how the different cell colonies arise,» Skupin asserts.

Finally, the researchers discovered that ROS generation subsided as the iPSCs matured, and these mature stem cell colonies survive best in a cellular environment with low levels of ROS.

No labeling was needed and we could, for the first time, perform detailed follow up of live, undisturbed cell colonies, and record all the events in the culture.

The automated monitoring system allowed a reliable area measurement of live, unlabeled, intact cell colonies.

A single co-transfection using two and four Cas9 / gRNAs enabled isolation of cell colonies harboring double and quadruple disruptions in 20 % and 2 % of colonies, respectively, which may not be easily achieved with ZFNs and TALENs technologies.

Genomic DNA was isolated from treated and wild - type cell colonies, or ear biopsies of cloned and wild - type goats.

72 h after transfection, cells were collected for RFLP assay (Mutation frequency analysis) or were seeded individually into 96 - well plates for isolating single cell colonies.

Cell colonies with MSTN biallelic mutations (MSTN - KO) were used for nuclear transfer.

In a study observing its effects on ovarian cancer cells and cell - mediated responses, Reishi inhibited cell colony formation and cell migration demonstrating its ability to protect already healthy cells.

To monitor larger areas or objects like cell colonies, a larger field - of - view (FOV) can be generated.

After only 10 days — instead of the more typical three to four weeks — one out of 100 hundred cells grew into a tiny colony with all the markings of a typical human embryonic stem cell colony.

«The organization of cell colonies and phenotypic switching between different types of colonies becomes a lot more flexible and rapid with reversible aneuploidy than if it depended on random mutations in the genes,» Skupin says.

As soon as the nervous system forming in the embryo begins to function as a whole — and not before — the cell colony begins to turn into a genuinely individual animal.

Nine blood stem cell colonies are illustrated in the image, which have developed into differentiated cell types, particularly into white blood cells (leukocytes).

Microscopic 10x view of a colony of embryonic stems cells (The stem cell colonies are the rounded, dense masses of cells.)

A grid - format picture capture system allowed the monitoring of living hESC cultures ranging in size from a few hundred micrometers to cell colonies of several millimetres at a defined picture capture cycle rate.

DNA sequencing further showed that these cell colonies included some small deletions in the MSTN gene (Figure 2B).

These images show human embryonic stem cell colonies, as grown in 1998 by researchers at the University of Wisconsin — Madison, in different stages of development.

The MID and LOW populations gave rise to some of these stem cell colonies, but formed more mixed or abortive colonies that were partially positive for GCTM - 2 than did the HIGH or NEG fractions.

In the LOW and NEG populations, very few cells were capable of forming stem cell colonies; most formed small colonies that were negative for GCTM - 2.

Causes and consequences of epigenetic changes, transcriptional profile modulation and new genetic profiles drive the single cell towards its destiny, contributing to the complexity of the multicellular organism or cell colony.

We replated cells on to a feeder cell layer and analyzed the formation of stem cell colonies fourteen days later by staining for expression of GCTM - 2 (Figure 4a, colony morphologies).