Sentences with phrase «cell culture environment»

Human ES and iPS cells grow as monolayers in a completely defined and xeno - free cell culture environment that enables self - renewal and pluripotency without the use of ROCK inhibitors.

Cell migration has mainly been studied in cell culture environment.

Your culture dishes could be infected with Mycoplasma, tiny bacteria that thrive in cell culture environments.

Not exact matches

Researchers from Duke University had previously used CRISPR to correct genetic mutations in cultured cells from Duchenne patients, and other labs had corrected genes in single - cell embryos in a laboratory environment.

The trophoblasts along with blood vessel cells were added to small dextran beads that were then spun around in a container filled with cell culture fluid, creating shear stress and rotational forces to better mimic the environment at the maternal - fetal interface than static cell - culture systems.

«We culture our cancer cells in this very low - oxygen environment, and they start behaving like they are inside a low - oxygen tumour.»

«In the last few years, it has become possible to order cultures of human cells for testing, but they're grown on a plate, a two - dimensional environment,» says Radisic.

The microbes rely on oxygen, carbon and other nutrients in their deep environment to live, but Røy's team found that carbon is so limited that the cells respire oxygen 10,000 times slower than bacteria in lab - grown cultures.

In the new study, the researchers cultured mouse skin - cancer colonies on various 2 - D and 3 - D environments of different shapes and patterns to see if the tumor shape contributes to activation of cancer stem cells, and to see where in the tumor the stem cells appeared.

Engineering a megakaryocyte niche (a special environment in which stem cells live and renew) that supports the growth of hematopoietic stem cells in culture, is the next step for the researchers.

«We've shown that you can make textured environments for cell culture fairly easily using graphene.»

Balaban agrees that the technique must be tested in animals to gauge its effectiveness in the body (as opposed to the less complex environment of a cell culture).

Haruko Obokata of the RIKEN Center for Developmental Biology (CDB) in Kobe, Japan, and colleagues at other Japanese institutions and at Harvard Medical School in Boston reported that simply subjecting blood cells from newborn mice to a moderately acidic environment for 25 minutes and then tweaking culture conditions could generate pluripotent stem cells capable of developing into nearly all of a body's cell types.

The team immersed the stem cells in four different types of these cultures and analysed which genes they expressed in which environment, and to what extent.

The protein comes from a culture of glial cells, whose normal job is to provide an environment in which nerve cells can thrive.

Moreover, a cell culture constantly alters its own environment — changing the concentration of oxygen, carbon dioxide and nutrients significantly over time.

AMSBIO produces 48 - page handbook for research scientists looking to culture cells in more physiologically relevant environments

This kit utilizes the DEF ‑ CS culture system, recognized for its suitability for genome engineering (Valton et al. 2014) and single - cell cloning (Feng et al. 2014), to promote reliable growth of hiPS cells in a feeder - free and defined environment.

The DEF - CS system is a robust culture system for efficient expansion of human induced pluripotent stem (iPS) cells in a feeder - free and defined environment.

For cell therapy, automation can be used to increase the scale of cell culture operations (e.g., bioreactors replacing flasks) and allow the use of closed systems that can protect cell products from contamination with adventitious agents from the environment or operators themselves.

7/16/2008 Improved Culture System for Hepatitis C Virus Infection A University of California, San Diego School of Medicine researcher has developed the first tissue culture of normal, human liver cells that can model infection with the Hepatitis C virus (HCV) and provide a realistic environment to evaluate possible... Culture System for Hepatitis C Virus Infection A University of California, San Diego School of Medicine researcher has developed the first tissue culture of normal, human liver cells that can model infection with the Hepatitis C virus (HCV) and provide a realistic environment to evaluate possible... culture of normal, human liver cells that can model infection with the Hepatitis C virus (HCV) and provide a realistic environment to evaluate possible... More...

culture (in microbiology) To grow cells outside the body or their normal environment, usually in a beaker, a laboratory dish or some big vessel.

culture (v. in microbiology) To grow cells outside the body or their normal environment, usually in a beaker, a laboratory dish or some big vessel.

The cultured cells were kept at 37 °C in a 95 % air / 5 % CO2 environment.

Although originally studied primarily in two - dimensional in vitro assays, most cell migrations in vivo occur in complex three - dimensional tissue environments that are difficult to recapitulate in cell culture or ex vivo Further, it is now known that cells can mobilize a diverse repertoire of migration modes and subcellular structures to move through and around tissues.

DT40 cells [27] were cultured in chicken medium (CM; DMEM / F - 12 supplemented with 10 % fetal bovine serum, 1 % chicken serum, 2 mM L - glutamine, 0.1 mM b - mercaptoethanol and penicillin / streptomycin) at 41 °C in a 5 % CO2 environment.

«What's missing when we put the cells in culture and compared to what's happening in the bone marrow environment in a living animal?

Cell culture studies involve removing tissue (or even individual cells) from a plant or animal and growing them in a laboratory environment.

The approaches include the co-cultivation of interdependent microorganisms, as well as gradient cultures and other methods mimicking the natural environment, and the exploitation of cell - to - cell communication.

Now Whitehead Institute researchers have designed a growth medium that more closely resembles the cells» environment in the body — and demonstrated that, relative to decades - old recipes that have remained the workhorses of cell culture studies, it significantly alters the cells» inner workings.

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