Sentences with phrase «cell culture for»

Plant physiologist and inventor M. Glen Kertz has been a global leader in the fields of molecular genetics, plant tissue and cell culture for over 35 years.

In order to move forward with developing a viable cell culture for genome editing we need to raise $ 60,000 for every 6 months or research until we have success.

They exposed the cells to proteins associated with TB and then analyzed the cell culture for expression of certain cytokines.

«We are moving into cell culture for the 21st century,» he says.

Over 20 days, albumin secretion in the enhanced hiPS - HEP occurs at comparable levels as in hphep cells cultured for a short time.

This year's winners include a fast way to analyze the chemical composition of cells, a suite of genetic tools to improve crops, and a method to screen images of 3 - D cell cultures for cancer cells.

Our team would like to be able to research passenger pigeon genes for de-extinction while primordial germ cell cultures are developed, and an Australian team has developed an effective way of bypassing cell cultures for engineering birds by going directly to the primordial germ cells in the embryo.

We can start by developing cell cultures for Domestic Rock Pigeons, which are readily available for research.

The «heart - on - a-chip,» which builds off previous successful iCHIP research on the peripheral and central nervous systems, involves the use of human cardiac cells cultured for up to nine days on the engineered chip.

Generation of dispersed presomitic mesoderm cell cultures for imaging of the zebrafish segmentation clock in single cells.

An equal number of MRL responder splenocytes were added and the cells cultured for 48 h before pulsing.

Not exact matches

Some reports, such as the Open Philanthropy Project's Animal Product Alternatives report and Van der Weele & Tramper (2014) suggest it is unlikely that cultured meats will become cost - competitive with conventional meat.98, 99 One important contributing factor in this conclusion, which is cited in these reports, is the minimum costs of the growth medium necessary for culturing the desired cells.

In fact, these dead starter culture cells and debris are an important food source for subsequent generations of microbes, referred to as non-starter lactic acid bacteria (NSLAB).

He has more than fifteen years» experience in the life science industry, including the product development, market development and commercialization of cells, scaffolds, cell reprogramming tools and cell culture media for regenerative medicine and bioprocessing applications.

EGFR / WT and KO cells were cultured overnight and starved for 24 h. Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -cells were cultured overnight and starved for 24 h. Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -Cells were lysed and cox - 2 mRNA levels were analyzed by RT - PCR.

In addition to managing the cell bank, the facility makes culture media and maintains a resale storeroom for laboratory products.

Shukla and colleagues discovered that a small drug molecule called BX795, which is sold to labs for use in experiments, helped clear HSV - 1 infection in cultured human corneal cells, in donated human corneas, and in the corneas of mice infected with HSV - 1.

Patent protection can also be sought for a new way to culture cells (a «method») or for a novel electrophoresis apparatus (a «machine»).

Setton's lab exposed the hiPSCs to a variety of different growth factors and culture media to coax them into first developing markers for, and then fully forming into, notochord cells.

For instance, to make a rod with collagen fibers aligned along its length (like a tendon) they cultured chondrocyte cells in a dog bone - shaped mold with loops on either end.

This study identifies myomerger as a fundmentally required protein for muscle development using cell culture and laboratory mouse models.

Despite being trapped and baked within a polymer, cell staining shows that the cultured cells remained alive, says Fernandez, an obvious necessity if they are to be used for artificial tissues.

The team pumped around 50 million rat liver cells into each of five bare scaffolds, then incubated the organs in culture for two weeks.

The Wyss team believes the ability of the human gut - on - a-chip to culture the microbiome with human gut cells also holds promise for the field of precision medicine, where a patient's own cells and gut microbiota could one day be cultured inside a gut - on - a-chip for testing different therapies and identifying an individualized treatment strategy.

With our human gut - on - a-chip, we can not only culture the normal gut microbiome for extended times, but we can also analyze contributions of pathogens, immune cells, and vascular and lymphatic endothelium, as well as model specific diseases to understand complex pathophysiological responses of the intestinal tract.»

The researchers — James Robl, a developmental biologist and his colleagues at the University of Massachusetts at Amherst, and Steve Stice at Advanced Cell Technology in Worcester, Massachusetts — inserted a marker gene fused with a gene for resistance to the chemical neomycin into a culture of connective tissue cells called fibroblasts.

Now researchers at the University of Plymouth, working in partnership with pharmaceutical company AstraZeneca, have for the first time successfully cultured and maintained cells from the guts of rainbow trout, a recommended fish species for toxicological studies.

«This research enables us to get one step closer to the optimal culture conditions for muscle stem cells.»

«If we win, the entire regulatory structure for autologous cell processing, with or without culture, will be rewritten such that any physician using good practices and treating patients responsibly can use stem cells as part of his or her medical practice,» he says.

New developments include reaction systems for catalysis research, sampling from reactors, systems for high - throughput applications in synthesis, the handling of microorganisms or cell - culturing procedures, and more.

To begin the research, the scientists screened human DIPG tumor cultures for surface molecules that could act as targets for CAR - T cells.

Also, researchers could carefully select which blood types to culture with each batch of stem cells, creating stockpiles of needed blood products for people with extremely rare blood types whose blood cell makeup makes it challenging to find good blood matches for transfusions because they would reject most other types of blood.

There will be several rounds of recruitment, and «specifically at this time, we are accepting applications for both junior / senior postdoctoral researchers and recent graduates wishing to enroll in our Ph.D. program as well as personnel with extensive expertise in cell culture and one expert in flow cytometry to fill in the position of manager of the flow - cytometry platform.»

Milk glands, moreover, do not need the constant coddling required for cell cultures.

One of the 12 audience members was Ben Chao, a chemical engineer with a Ph.D. — not at all qualified to make a judgment on Scott's abilities in the area of «Approaches to Developing Serum - Free Media Formulations for Mammalian Cells in Culture.»

Although such harvested cells could be cultured as say, liver cells for treating hepatitis or dopamine - producing cells for Parkinson's, the resulting transplants would likely be rejected by patients» immune systems.

For example, cultured cells exhibit rhythmic oscillations in gene expression where there is no input from the master clock.

As it can take weeks to grow human cells into intact differentiated and functional tissues within Organ Chips, such as those that mimic the lung and intestine, and researchers seek to understand how drugs, toxins or other perturbations alter tissue structure and function, the team at the Wyss Institute for Biologically Inspired Engineering led by Donald Ingber has been searching for ways to non-invasively monitor the health and maturity of cells cultured within these microfluidic devices over extended times.

Although the bulk of the commercial manufacturing uses cultures of bacteria, such as Escherichia coli or Chinese hamster ovary cells, a few biotech companies are trying to produce therapeutic proteins in the milk of transgenic mammals (such as GTC Biotherapeutics, which is using goats; PPL Therapeutics, which is using sheep; and BioProtein Technologies, which is working with rabbits), transgenic chicken eggs (such as Avigenics or Vivalis), or even in transgenic crops (such as ProdiGene or Meristem Therapeutics); but it is early days for these «pharming» methods.

Oncologists William Hahn, Robert Weinberg, and colleagues at the Whitehead Institute for Biomedical Research in Cambridge, Massachusetts, mutated the gene for one part of the enzyme and inserted it into cultured human cells from colon, ovary, and breast tumors.

Sicor, for example, has recently acquired a plant in Vilnius, Lithuania, and Genentech a cell culture manufacturing facility in Porriño, Spain.

If it proves positive, the researchers will mix another portion of the sample with cultured bat and monkey cells to test for active virus.

The students used a 3 - D printer to design a smaller and more cost - efficient bioreactor — a miniaturized culture device where neural stems cells are grown and eventually become small laboratory brains for research.

The enzyme, called tankyrase, may prove useful for extending the lives of cultured cells grown to repair burned skin and other damaged tissue.

If a breast cancer line had silently contaminated Klonisch's cell culture early on, it would have been subject to the usual technique for immortalizing a normal cell (which involves applying enzymes, antibiotics and antibiotic - resistant genes).

Until recently, Ain was renowned for a highly prized repository of 18 immortal cancer cell lines, which he developed by harvesting tissue from his patients» tumors after removal, carefully culturing them to everlasting life in vials.

In addition, cell culture studies lasted two days in Gentzsch's study, whereas the clinical studies lasted for months.

Researchers have videotaped cultures in which embryos develop but found no visual pattern that hints at which cells are about to sprout, and staining for certain patterns of gene expression has been inconclusive.

Existing gels for cell culture are often animal derived, which can affect the reproducibility of results or, if synthetic, require coatings or coupling of cell - adhesive ligands.

In 2009, from experiments at the RIKEN Center for Developmental Biology in Kobe, Japan, he found that when culture conditions are right, adding a single ingredient — bone morphogenetic protein 4 (Bmp4)-- with precise timing is enough to convert embryonic cells to PGCs.