Sentences with phrase «cell culture medium»

Moreover, the interfering effect of the cell culture medium is significantly reduced.

Inset corresponds to high resolution image showing d lattices; (B) Energy dispersive X-ray analysis showing Ag but no impurities; (C) zeta potential of different prepared samples; (D) DLS of samples in cell culture medium showing well - dispersed nanoparticles.

Most of DLS measurements were carried out at 37 °C (to simulate physiological temperature) by use of a Malvern Zeta Nanosizer in water and in cell culture medium (DMEM + 10 % FCS).

To measure the pH of our samples, we used a solution of cell culture medium, which is buffered and contains the pH indicator phenol red.

When they put human macrophages in the lower compartment filled with cell culture medium, the cells rose toward the membrane.

Previous work by the researchers developed plasma - activated cell culture medium as a form of chemotherapy, but selected Ringer's solution in the present work because of its simpler composition and likelihood of forming less complex reaction products.

Not exact matches

Some reports, such as the Open Philanthropy Project's Animal Product Alternatives report and Van der Weele & Tramper (2014) suggest it is unlikely that cultured meats will become cost - competitive with conventional meat.98, 99 One important contributing factor in this conclusion, which is cited in these reports, is the minimum costs of the growth medium necessary for culturing the desired cells.

«When someone has a problem — whether they need to find a particular cell line or need to understand the formulation of a particular type of culture medium — they're coming to you and they're in a crisis.

Adding ascorbic acid to culture medium could help overcome the main roadblock in reprogramming human, mouse cells into iPS cells

In addition, mammalian cell cultures are not always an ideal medium: at times, it is simply too hard to produce proteins in this manner.

To find out, they put droplets of culture medium that cancer cells had grown in on one side of petri dishes.

When the Stanford team measured pyrophosphate levels in cultured cells derived from ank and normal mice, they found that the chemical accumulated in cells from the ank mice but decreased in the culture medium.

The cells are grown in the incubator in different concentrations in a culture medium.

Diluted and filtered liquid medium drawn from the fibroblast cell culture also killed breast and lung cancer cells.

Hang and coworkers exposed the human cells by first extracting the compounds from the paper with a culture medium then using the medium to culture the human cells for 24 hours.

Although electrolyte leakage is still undesired, its danger is minimized by the use of either the normal saline solution pumped into the body in most IV treatments or a cell - culture medium that contains amino acids, sugars, and vitamins in addition to sodium ions and thus mimics the fluid that surrounds human cells.

Researchers in China have engineered bendable batteries that can run on body - inspired liquids such as normal IV saline solution and cell - culture medium.

To shock the plants, Cuello applied a 30 to 100 milliamp current to the growth - medium of plants grown hydroponically, or, in the case of barrel medic, to the solution surrounding the cell cultures.

Their stem cells, which can mature into any type of tissue, were isolated and cultured in a dish around dots of gel - like growth medium.

Following cultivation of Arabidopsis seedlings on a culture medium containing a high concentration of cesium carbonate, Cesium Green was applied to the seedlings, and the resulting green fluorescence observed was used to confirm the presence of cesium within the plants» cells.

One possible pilot project would create human cells that can tolerate a simpler and less costly culture medium by surviving without the typical amino acids and vitamins.

The group reported growing multiple parthenogenetic embryonic stem cell lines by incubating eggs in a warm, low - oxygen culture medium.

The cells were cultured in a suitable medium and the researchers checked the molecules they produced, in particular the pro-angiogenic ones, that is, molecules that nurture the production of new blood vessels.

The kit adopts a novel purification method, using magnetic beads and phosphatidylserine - binding protein to isolate exosomes and other EVs from cell - culture medium and body fluids by a normal microcentrifuge.

When NGF is withdrawn from the culture medium, the cells retract their fibers, lose their other neuronal properties and resume the uncontrolled proliferation characteristic of neoplastic cells.

EGCG or DAC was added, in new culture medium, to the cells on days 1, 3, and 5.

Spleen cells (3 × 107) from either experimental or control effector lymphocytes were dispensed into 75 - cm2 tissue culture flasks along with 20 ml of boosting medium and 6 × 105 γ - irradiated (3,000 rad) BALB / c spleen cells (erythrocytes lysed).

Human iPS cell - derived hepatocytes differentiated with our robust differentiation protocol and cultured using our novel maintenance medium provide an inexhaustible, consistent supply of functional hepatocytes that can be used to advance the understanding of diseases related to dysfunction in liver metabolism, including NAFLD / NASH, type 2 diabetes, and metabolic syndrome.

To address this question, we plated these cells on microwells, transduced the cells one day after and then switched to a neuronal culture medium as low oxygen.

To address this problem, we developed a robust hepatocyte differentiation protocol — which promotes a highly synchronized differentiation pattern across multiple hiPS cell lines [1]-- and a novel maintenance medium, which enables the long - term culture of our hiPS cell - derived hepatocytes.

Human iPS cell - derived hepatocytes differentiated with our robust differentiation protocol and cultured using a novel maintenance medium provide an inexhaustible, consistent supply of functional hepatocytes that can be used to advance the understanding of diseases related to dysfunction in liver metabolism, including NAFLD / NASH, type 2 diabetes, and metabolic syndrome.

Accumulation of lipids to their maximum level occurred when cells began to synthesize amino acids in the presence of excess glucose - the major carbon source in the culture medium.

Daily medium exchanges help alleviate cellular stresses associated with pH changes and resource consumption due to the high cell density of the culture.

Traditionally, hMSCs have been cultured on two - dimensional cell culture platforms using serum - containing medium.

StemFit Basic02 is a xeno - free, defined medium for human pluripotent stem cell (hiPSC) culture that offers an effective solution for regenerative medicine research.

Then, cells were either stimulated with methionine - choline deficient media (MCD - media; WME without choline, methionine, or glutamine and with 0.1 % BSA) for 24 hr or cultured in control medium (WME with 0.1 % BSA).

Once isolated these cells can be grown in a liquid medium of nutrients, called culture.

This group cultured human CD34 + cells in StemSpan ™ medium and cytokines as a pre-stimulation step ahead of electroporation, before xenotransplantation into mice or additional in vitro culture.

Delaney et al. [4] who have shown that cord blood - derived CD34 + cells cultured in StemSpan ™ medium with cytokines, on plates coated with Notch ligand, are expanded ex vivo while still maintaining their ability to engraft into immunocompromised mice.

None of these toxic effects were observed when the same cells were treated with culture medium from non-senescent astrocytes that had not been subjected to paraquat.7

MDA - MB 231 and BCM2 cell lines were cultured in minimum essential medium (MEM)(Invitrogen Corporation, Carlsbad, CA, USA) supplemented with 10 % fetal bovine serum (HyClone, Logan, UT, USA; Thermo Fisher Scientific Inc., Waltham, MA, USA), 2 mM L - glutamine, 1 mM sodium pyruvate, 1 mM non-essential amino acids, and 1 % vitamin (HyClone).

Before determining glucose uptake, all cell cultures were exposed to basal medium for 1 h. Cultures used for acute insulin stimulation were exposed for 1 h to high insulin as described for GScultures were exposed to basal medium for 1 h. Cultures used for acute insulin stimulation were exposed for 1 h to high insulin as described for GSCultures used for acute insulin stimulation were exposed for 1 h to high insulin as described for GS assays.

After 2 — 3 weeks, propagated cells were dissociated with 0.25 % trypsin, resuspended with culture medium, and cultured for a further 2 — 12 weeks.

Expansion of CD34 + cells normalized relative to the values obtained in SFEM medium (dark gray bar) after culturing purified CD34 + cord blood cells for 7 days in StemSpan ™ serum -[SFEM, SFEM II (gold bar)-RSB- and animal component - free ACF (orange bar) media, and six media from other suppliers (light gray bars).

By culturing cells in a fed - batch system with StemSpan ™ medium containing cytokines and UM171, expanded cells were found to successfully engraft and repopulate immunocompromised mice, with no disadvantage when compared to unmanipulated cells.

To determine the effect of the conditioned medium on NGF - induced neurite outgrowth, PC12 cells were dissociated and plated on polyornithine - coated tissue culture dishes in the following conditions: 1) HT22 conditioned medium, 2) J147 treated HT22 conditioned medium, 3) DMEM alone plus J147, 4) DMEM plus NGF at 50 nanograms per ml, 5) J147 treated HT22 conditioned medium pre-incubated for one hour with 10 μg / ml anti-NGF and N2 supplement (Invitrogen).

Thirdly, HFF cells are more tolerant to certain reagents added to the culture medium.

Bone marrow cells cultured with conditioned medium derived from ABL1 / ABL2 knockdown 1833 and SKBR3 breast cancer cells had decreased numbers of TRAP + cells compared to the control groups (Fig. 5, B and C, and fig.

For induction and culture of mouse iPS cells, HFF cells were passaged using trypsin and cultured in medium containing DMEM, 10 % FBS (Hyclone), 1 % NEAA (Invitrogen), 2 mM L - glutamine (Invitrogen), 100 U / ml penicillin and 100 µg / ml streptomycin.

Briefly, NHLF cells were grown in 96 - well CoStar tissue culture plates (4,000 cells per well) and either subjected to siRNA gene silencing or directly treated with compounds for 51hours in 100 µL complete medium per well.