Sentences with phrase «cell culture plates»

The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and cultured at 37 °C in a 5 % CO2 incubator.

This protocol video demonstrates how to surface - coat cell culture plates and transwell inserts with Cultrex ® Basement Membrane Extract, also called Cultrex ® BME.

Not exact matches

But if the quantity of the protein was reduced slightly, the culture yielded a mixture of floor plate cells and dopamine neurons (Neuron, vol 15, p 35).

Companies making high - content cell imaging equipment such as confocal microscopes and plate readers are now adding software tools to process images of 3D cell cultures.

I found that culturing cardiac valve cells on synthetic hydrogels preserves their normal properties better than the traditional plastic plates do, and I discovered a signaling pathway connecting the stiffness of the supporting scaffold to the cells» cytoskeletal structure.

«In the last few years, it has become possible to order cultures of human cells for testing, but they're grown on a plate, a two - dimensional environment,» says Radisic.

After collecting biopsies from banked human donor eyes, the team expanded the numbers of cells in a culture plate using human serum to nourish them.

The research highlights the importance of studying the effects of drugs not only on the tumor cells in a culture plate, but also on the interaction of the tumor cells with their own microenvironment.

If the cells grew on culture plates, the team inferred that the human gene could fill in for its yeast equivalent.

The researchers removed the organ of Corti, which is housed within a seashell - shaped cavity called the cochlea and contains sensory hair cells, from newborn mice and kept the cells alive in culture plates.

Expanding from their previous studies with mice, the researchers first established that under specific conditions, culturing human embryonic stem cells with fibroblast growth factor 2 (FGF2) leads to neural differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of markers for the cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.

COS - 7 cells, plated at 70 % confluence in 100 - mm tissue culture dishes, were transfected with vector alone or pcDNA3 - ACVR1 (c. 617G) or pcDNA3 - ACVR1 (c. 617A).

Total RNA was extracted from frozen adipose tissue (100 mg), FACS - isolated cells (> 105), or cultured cells (60 - mm confluent plate) using a commercially available acid - phenol reagent (TRIzol; Invitrogen Corp.).

D, the same primary breast cancer cells infected with KLF4 or control lentivirus were cultured in low - attachment plates and the number of mammospheres was counted after 10 days.

The tumor cells were dissociated and the cells were infected with KLF4 lentivirus and cultured in low - attachment plates.

C, primary breast cancer cells isolated from patients with advanced cancer were directly infected with KLF4 or control lentivirus, and the CSC population (CD24 − CD44 + ESA +) was measured by FACS after culturing the cells for 72 hours in low - attachment plates.

This demonstrates that even though the anterior neural plate is essential for proper eye morphogenesis, cultured pluripotent cells were not directed to a retinal fate — even when grafted directly into the eye field.

For intracellular cytokine staining, lymphocytes were cultured in 96 - well flat - bottom plates at 1 × 106 cells / well in 200 μl RPMI 1640 supplemented with 10 % FBS in the presence or absence of GP33, GP276, and NP369 peptides.

This process (see figure) started with spin - embryoid body (spin - EB) formation from cultures of single - cell hiPSC suspensions in 96 well plates, and the subsequent addition of BMP4 and FGF - 2 to induce mesoderm differentiation, and then VEGF and SCF to induce hematopoietic differentiation.

To address this question, we plated these cells on microwells, transduced the cells one day after and then switched to a neuronal culture medium as low oxygen.

Thirdly, the current, almost universal dependency of organoid culture on the use of Matrigel as a replacement for the function of the extracellular basement matrix in providing structural support and survival signals to the epithelial cells hampers clinical application, considering its origin from a mouse sarcoma cell line, its poorly defined composition and its mechanical rigidity after plating.

Like a conventional tissue culture plate, DA - HCS plates can be easily coated with DNA, poly - lysine, Collagen, fibronectin, silane - compatible substrates, or any other cell culture coatings.

First, cells from Cellartis Human iPS Cell Line 22 (ChiPSC22) were modified to stably express AcGFP1, then these cells were cultured using the DEF ‑ CS culture system in a 48 - well plate.

Delaney et al. [4] who have shown that cord blood - derived CD34 + cells cultured in StemSpan ™ medium with cytokines, on plates coated with Notch ligand, are expanded ex vivo while still maintaining their ability to engraft into immunocompromised mice.

For cell proliferation assay, 5 × 104 cells were seeded in triplicate wells per cell line per time point in 12 - well tissue culture plates.

To determine the effect of the conditioned medium on NGF - induced neurite outgrowth, PC12 cells were dissociated and plated on polyornithine - coated tissue culture dishes in the following conditions: 1) HT22 conditioned medium, 2) J147 treated HT22 conditioned medium, 3) DMEM alone plus J147, 4) DMEM plus NGF at 50 nanograms per ml, 5) J147 treated HT22 conditioned medium pre-incubated for one hour with 10 μg / ml anti-NGF and N2 supplement (Invitrogen).

Unfortunately, inclusion of the 2i in our culture system resulted in massive death of irradiated MEF cells plated at a relatively low density, suggesting that the 2i strategy might not be compatible with irradiated MEF cells.

For intracardiac injections, cells were harvested from subconfluent culture plates, washed with phosphate - buffered saline (PBS), and resuspended at 106 / ml (1833) or 5 × 106 / ml (SCP28) in PBS; 0.1 ml of the suspended cells was injected into the left cardiac ventricle using 30 - gauge needles.

Briefly, NHLF cells were grown in 96 - well CoStar tissue culture plates (4,000 cells per well) and either subjected to siRNA gene silencing or directly treated with compounds for 51hours in 100 µL complete medium per well.

Confluent HFF cells were treated with irradiation of 55 Gy and plated onto culture dishes (1 × 105 per 2.89 cm2).

Sorted human ILC3s (Lin - CD127 +) were cultured at 2,000 — 5,000 cells per well of a 96 well plate with irradiated (30 Gy) OP9 - DL1 feeders [70].

In this instance we did not detect a plating difference in the populations and approximately 16 % of the deposited cells survived to give rise to day 10 cultures (unpublished data).

A single - cell suspension (50 μl) containing 1500 cells was mixed with Matrigel (1:1) and plated on top of the Matrigel base onto wells of a 96 - well plate; 50 μl of complete medium was added, and the cells were cultured for 14 days.

F1 virgin females were collected, transferred to individual cells of plastic 24 - well culture plates (various manufacturers) and given a single fly host to lay eggs.

«Before, you could in principle test chemical compounds of interest against hundreds of cell lines, but it would require a lab member going to a freezer, taking out hundreds of vials — one for each cell line — and setting up hundreds of tissue culture plates.

Cells were plated in 12 - well plates at 0.8 × 105 cells / well (A427) or 1.8 × 105 cells / well (H2122) in RPMI medium containing 10 % FBS and cultured overnCells were plated in 12 - well plates at 0.8 × 105 cells / well (A427) or 1.8 × 105 cells / well (H2122) in RPMI medium containing 10 % FBS and cultured overncells / well (A427) or 1.8 × 105 cells / well (H2122) in RPMI medium containing 10 % FBS and cultured overncells / well (H2122) in RPMI medium containing 10 % FBS and cultured overnight.

NHLF cells were cultured in 96 - well Optilux plates as described in Materials and Methods.

Inverted microscopes are used to image lives cells in culture (flasks, dishes, or multi-well plates) using transmitted light, phase contrast, DIC, or fluorescence modes.

Cells were plated in six - well plates at 2.5 × 105 (A427) or 4 × 105 (H2122) cells / well in RPMI 1640 medium containing 10 % FBS and cultured for 24 h. Culture medium was collected, and PGE2 levels were determined using a PGE2 EIA kit (Cayman ChemicCells were plated in six - well plates at 2.5 × 105 (A427) or 4 × 105 (H2122) cells / well in RPMI 1640 medium containing 10 % FBS and cultured for 24 h. Culture medium was collected, and PGE2 levels were determined using a PGE2 EIA kit (Cayman Chemiccells / well in RPMI 1640 medium containing 10 % FBS and cultured for 24 h. Culture medium was collected, and PGE2 levels were determined using a PGE2 EIA kit (Cayman Chemicals).

Cells were cultured for 10 d following plating and 12 wells derived from each fraction were subjected to flow cytometry.

(A) BaF3 cells control or stably transduced with wild - type or with V617F, Y931C or V617F / Y931C mutant were washed 3 times in PBS to remove the residual IL - 3 and seeded in 6 - well plates at a concentration of 100,000 cells / mL in culture medium.

The cells were then seeded into 12 - well culture plates (1 ml / well; Falcon, Franklin Lakes, NJ).

Cells were then plated in 24 - well tissue culture treated plates and incubated in a humidified 5 % CO2 incubator at 37 °C.

Bulk - cultured PC3 - GFP cells (A), holoclone P1H8 (B)-- , or meroclone P2H3 (C)-- derived cells were plated on glass coverslips and stained for CD44, α2β1, and / or β - catenin using monoclonal antibodies.

For induction of differentiation to mature endothelial cells, EPCs were plated at a high cell density (8 × 104 cells / cm2) on tissue culture treated flasks.

Cells were cultured in 21 % or 1 % oxygen for 48 hours following transfection and then plated in mammosphere culture.

Bulk - cultured PC3 or PC3 - GFP cells, or clone - derived cultures were plated on glass coverslips and used in immunofluorescence staining for CD44, α2β1 integrin, and β - catenin using monoclonal antibodies and protocols described previously (27 — 29).

Sorted cells cultured in 48 - well plates for 17 days were washed once with PBS and fixed for 5 min.

A, following 48 hours in 21 % or 1 % oxygen, ± YC1 cells were plated in mammosphere culture.

To eliminate the possibility of any undifferentiated cells in the cultures accounting for this observation, DMSO - differentiated cells were treated with the anti-proliferative agent cytosine arabinosidase (Ara - C), starting two days after aggregate plating.