Sentences with phrase «cell culture results»

This unique workshop provides the introduction and practical hands - on of integrated metabolomics workflows, employing the techniques of cellular energy metabolism (Agilent Seahorse XF), detailed metabolomic analysis by LC / MS Q - TOF and the translation of cell culture results into a living mouse model using indirect calorimetry.

Institut Pasteur scientists are now testing additional samples from the 24 patients and waiting for cell culture results.

To replicate these cell culture results, Rani used human stem cells to grow neurons into what is called a mini brain.

Not exact matches

CCG is also developing cell lines that are cultured to represent tumor behavior in vivo, and computational methods that will help researchers understand tumor behavior and apply the resulting data to diagnoses, treatments, and cures.

Yet Pereira - Smith, who still pops into the lab periodically to do her own cell culture work, was always available to discuss results and offer encouragement.

Although such harvested cells could be cultured as say, liver cells for treating hepatitis or dopamine - producing cells for Parkinson's, the resulting transplants would likely be rejected by patients» immune systems.

«It's possible that the culture conditions are causing increased stress on the cells, resulting in cell death.»

«This small change in injection protocol has resulted in something better than all prior embryo work and cell culture work,» Church says.

In the study, the researchers show that after two to four weeks of 3 - D culture, the resulting muscle cells form muscle fibers that contract and react to external stimuli such as electrical pulses and biochemical signals mimicking neuronal inputs just like native muscle tissue.

Existing gels for cell culture are often animal derived, which can affect the reproducibility of results or, if synthetic, require coatings or coupling of cell - adhesive ligands.

As a result, 3D cell culture systems have become a staple of cell biology.

The implications were hardly lost on the Bethesda crowd: If the virus was transmitted in cell cultures in Ruscetti's lab, it could also be contaminating the nation's blood supply as a result of blood donations from unknowingly infected donors.

An APC - mimetic scaffold that was engineered to activate a specific type of CAR - T cell was able to generate higher numbers of the modified T cells over longer periods of culture than analogously designed expansion beads, and the resulting cells were similarly effective in killing the lymphoma cells in the mice.

Results suggest that cells grown in the device exhibit more natural behaviors than when grown in traditional culturing methods, and the filtration by the glomerulus is necessary for healthy cell function.

To test the suitability of guanabenz as a potential treatment for MS, Popko and his team exposed cultured oligodendrocyte cells to interferon gamma — a molecule that increases inflammation — resulting in greatly increased myelin loss and cell death.

Together, Raman says, this dual - mode treatment produced cell - killing results that paralleled their experiments in cell cultures.

An experimental drug that targets abnormally high levels of a protein linked to cancer growth appears to significantly reduce the proliferation of prostate cancer cells in laboratory cell cultures and animals, while also making these cells considerably more vulnerable to radiation, according to results of a study led by Johns Hopkins scientists.

Following cultivation of Arabidopsis seedlings on a culture medium containing a high concentration of cesium carbonate, Cesium Green was applied to the seedlings, and the resulting green fluorescence observed was used to confirm the presence of cesium within the plants» cells.

The resulting five - ounce burger, cooked by chef Richard McGeown, was made using 20,000 strips of cultured meat — about 40 billion cow cells — and took about three months to produce, «which is faster than [raising] a cow,» Post joked.

Experiments on cell cultures from patients with malignant melanoma confirmed the new results.

«Our results suggest that megakaryocytes might be used clinically to facilitate adult stem cell regeneration and to expand cultured cells for adult stem cell transplants,» says Meng Zhao, Ph.D., a postdoctoral fellow at Stowers and lead author on the study.

Blocking the activity of IL - 4 and IL - 13 in the cultured skin cells resulted in an abundance of long - chain lipids.

All human cell lines growing in tissue culture dishes, and about 95 % of cells in a human being, split evenly, resulting in daughter cells of equal size.

The results showed: cell death increased in relation to the particle dose with all cell cultures.

Further testing of this culture method on kidney progenitor cells derived from human iPS cells resulted in four times the total amount of cells over 8 days.

The team rewrote the Microsoft formal verification program, then fed genetic and chemical data from the different stem cell cultures into it — with some surprising results.

«The resulting hydrogel composed of physically self - assembled CarHC polymers exhibited a rapid gel - sol transition on light exposure, which enabled the facile release / recovery of 3T3 fibroblasts and human mesenchymal stem cells (hMSCs) from 3D cultures while maintaining their viability.»

This fact makes the LCSB scientists» results of extraordinary interest to Parkinson's researchers worldwide, as Schwamborn stresses: «On our new cell cultures, we can study the mechanisms that lead to Parkinson's much better than was ever the case before.

So the researchers needed cells that divided rapidly and, as a result, survived longer in culture.

Considerable previous research in cell cultures has demonstrated that low doses of ionizing radiation results in «bystander» effects, in which nearby, unexposed tissues suffer cell death, mutations, and tumor - inducing growth (ScienceNOW, 7 September 2005).

They show that highly purified NS1 acts as a pathogen - associated molecular pattern (PAMP) that activates mouse macrophages and human peripheral blood mononuclear cells (PBMCs) in culture via TLR4, resulting in release of inflammatory cytokines — an effect that was blocked by either a TLR4 antagonist or an anti-TLR4 antibody.

An oral drug used to treat an illness unrelated to HIV eradicated infectious HIV - producing cells in lab cultures while sparing uninfected cells — and suppressed the virus in patients during treatment and for at least eight weeks after the drug was stopped, according to results of a clinical pilot trial and researchers at Rutgers University and Dartmouth College.

Overexpression of wild - type ACVR1 in chick micromass cultures resulted in expression levels of marker genes that are comparable to those of the empty viral vector, while cells overexpressing R206H ACVR1 showed upregulation of collagen type II and aggrecan.

Similar to the results obtained with cultured human cells, the transgenic mice were resistant to avian influenza viruses but susceptible to flu viruses of human origin.

Their results, published in Emerging Infectious Diseases, demonstrate that the highly differentiated 3 - D cell culture model can support the natural growth of human noroviruses, whereas previous attempts using differentiated monolayer cultures failed.

Overgrown cultures stimulate quiescence, the resting phase of the cell cycle; quiescent cells do not take up nucleic acid or express transgenes as well as actively dividing cells resulting in lower lentiviral titers.

These results provide the validity to the newly developed hypoxia / reoxygenation culture system for examining the regulation of CSCs in breast cancer cell lines by niche factors in the tumor microenvironment and developing differential targeting strategies to eradicate breast CSCs.

(10) The company's scientists also report that when they then injected preformed AS fibrils into the brains of mice, BIIB054 slowed the self - templating spread of AS pathology across the brain (consistent with their cell - culture study), and held much of the ensuing motor dysfunction at bay (9,10)(reducing it «by more than 50 percent» according to a science journalist who was present when they presented results at AD / PD 2017 (11)-RRB-.

Unfortunately, inclusion of the 2i in our culture system resulted in massive death of irradiated MEF cells plated at a relatively low density, suggesting that the 2i strategy might not be compatible with irradiated MEF cells.

We combine it with the use of human tissue culture cells in which we validate some of the results obtained in egg extract.

This portable instrument equipped with user - friendly touchscreen provides reliable result throughout the cell culture workflow and accelerates the cell culture process in a matter of seconds.

Our results demonstrated that our 3D cell culture device enables a real - time imaging of giant vacuole formation and tracers crossing the cultured endothelial cell monolayer in a controlled experimental condition.

Results from this Shaffer Grant study also show that targeting antioxidants such as XJB -5-131 to the TM significantly attenuates expression and release of TGF - β2 from cultured human TM cells.

As expected the culture of HV cells under these conditions resulted in a significant reduction in the V+S + population (Figure 6B).

Importantly, cisplatin toxicity and Pgp efflux transporter activity measured on - chip more closely mimic the in vivo responses than results obtained with cells maintained under conventional culture conditions.

Researchers performed ES cell cultures, RNA preparations and validated microarray data: IA KA NB PYB MXD MG EK SL MR AR MPS MT. Results were discussed among all Consortium partners.

Results: During in vitro culture period, the percentages and absolute numbers of T cells expressing the CARs containing a hinge domain continuously increased, mainly through the promotion of CD4 + CAR T cell expansion, regardless of the single - chain variable fragment (scFv).

Specifically, we will spend your generous donations on cell culture reagents, oxygen consumption measurements, virus production, quantitative reverse transcription PCR, DNA synthesis services, and publication of our results in a peer - reviewed journal.

The flow cytometry results showed a quantitative continuous gradient of stem cell antigen expression in ES cell cultures, a gradient that reflected the position of cells in a colony relative to the feeder cell layer.

These results support data suggesting that excess HS oligosaccharides impair function of cellular adhesion molecules and disrupt normal polarization and orientation of cultured MPSIIIB mouse astrocytes or neural stem cells [52].