Sentences with phrase «cell culture supernatant»

However, despite multiple serial passages, we were unable to propagate the infected cell culture supernatant in either BSC - 1 or PMK cells.

In this study, we have developed a new sensitive and specific one step rRT - PCR for detection of ZIKV in serum and cell culture supernatants using a Taqman probe containing locked nucleotides, in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and Asia.

The enveloped viral particles were also observed in negatively stained samples prepared from the concentrated cell culture supernatants and allantoic fluids (Fig. 4F).

For cell culture supernatant, levels of IFN - γ and IL - 17A were measured by ELISA, according to the manufacturer's instructions (R&D Systems).

Target detectability data from 858 samples from 11 independent customer sites, utilizing five unique sample types: serum, plasma, cell culture supernatant, lysates, and bronchoalveolar lavage.

Allantoic fluid and the cell culture supernatants from the first five passages were tested for hemagglutinating activity with a 1 % suspension of chicken erythrocytes to exclude avian hemagglutinating viruses (e.g., influenza virus and EDS - 76 virus).

Insets are magnified virions (E); Negative staining of the purified BYDV from the cell culture supernatants (arrow indicates the typical enveloped flavivirus)(F).

The cell culture supernatant was collected and concentrated if necessary, and then 100 μL aliquots were used for detection with ELISA kit (PBL Biomedical Laboratories) following the protocol of the manufacturer.

The cell culture supernatant was collected and cytokine secretion was determined using the Mouse IFN - gamma Quantikine ® ELISA Kit (Catalog # MIF00), the Mouse IL - 4 Quantikine ® ELISA Kit (Catalog # M4000B), and the Mouse IL - 17 Quantikine ® ELISA Kit (Catalog # M1700).