Sentences with phrase «cell culture supernatants»

In this study, we have developed a new sensitive and specific one step rRT - PCR for detection of ZIKV in serum and cell culture supernatants using a Taqman probe containing locked nucleotides, in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and Asia.

Angiopoietin - 2 protein levels of cell culture supernatants were measured by ELISA (R&D, #DANG20) according to the manufacturer's instructions.

Target detectability data from 858 samples from 11 independent customer sites, utilizing five unique sample types: serum, plasma, cell culture supernatant, lysates, and bronchoalveolar lavage.

Vaccinia virus (Western Reserve) recombinant for the codon - optimised gp120 gene from HIV - 1 (Bx08) expressing gp120 protein in (VERO) cell culture supernatant.

The cell culture supernatant was collected and concentrated if necessary, and then 100 μL aliquots were used for detection with ELISA kit (PBL Biomedical Laboratories) following the protocol of the manufacturer.

The cell culture supernatant was collected and cytokine secretion was determined using the Mouse IFN - gamma Quantikine ® ELISA Kit (Catalog # MIF00), the Mouse IL - 4 Quantikine ® ELISA Kit (Catalog # M4000B), and the Mouse IL - 17 Quantikine ® ELISA Kit (Catalog # M1700).

Not exact matches

After achieving 80 — 90 % confluency, cell culture media were changed to maintenance media with 2 % FBS and were inoculated with 200 µL of clinical sample or 100 µL of passaged viral supernatant.

Mcc was purified from the culture supernatants of E. coli VCS257 cells harboring pJEM15 plasmid, as described earlier (Bieler et al., 2005).

UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.

Cell debris was removed from culture supernatant by centrifugation at 12,500 g for 45 min at 4 °C, and the virus particles were concentrated by ultracentrifugation at 45,000 r.p.m in a BECKMAN 70Ti rotor for 3 hrs.

Assessment of hASC - CM composition found high expression of various human growth factors (IL ‐ 6, 8, 12, eotaxin, IP10, MCP ‐ 1, VEGF, and TIMP ‐ 1) in the supernatant following the co-culture of hASCs with islet cells, while IP10, eotaxin, VEGF, and TIMP ‐ 1 became increased with time during islet co ‐ culture, suggesting the presence of paracrine cross ‐ talk between islets and hASCs.

To characterize the isolate that replicated in HeLa cells, a 166 - bp RNA sequence containing the variable region C of the envelope (Env) surface protein was PCR - amplified from infected HeLa cell tissue culture supernatants.

Cells were cultured under normal culture conditions for 24 h and the supernatants were subjected to PGE2 ELISA.

Because ATRA could cause IFNα synthesis and secretion in NB4 cells by up - regulating IRF - 1 (23), we thus quantitated the IFNα in culture supernatant of ATRA - treated NB4 cells.

After 24 hours (on day 1) the mixed viral supernatant was removed, the cells were washed twice with PBS and then cultured in fresh MEF medium.

UNISI will provide expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.

0.1 mL of supernatant was added to BHK - 21 and African green monkey kidney (Vero) cell cultures (ATCC, Manassas, VA) and monitored as above.

On day 3 the mixed viral supernatant was again removed, the cells were washed twice with PBS and then cultured in fresh MEF medium.