Sentences with phrase «cell culture system»

This study used a model cell culture system that represents what happens in an animal or human.

Our goal is to create a microfluidic 3D cell culture system representing a «placenta - on - a-chip» in order to mimic the nutrient / waste transfer between maternal blood and fetal blood that occurs in the cotyledon section of the placenta, and to test and observe the effects of ethanol within the maternal bloodstream and compare it to a similar in vivo situation.

Recruiting Volker was one of the biggest strokes of luck in my scientific life as he was not only the key person to set up the first HCV cell culture system, but also an exceptional mind creating true team spirit and synergy within the research team.

In the absence of GFP, no reporter output was observed in their 293 cell culture system.

The laminin - 521 stem cell matrix is a feeder - free, chemically defined and xeno - free cell culture system that support high cell survival and fast proliferation of hESC and iPSC.

Laminin - 521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno - free and biologically relevant cell culture system.

Gelatine methacrylamide - based hydrogels: An alternative three - dimensional cancer cell culture system.

It facilitates self - renewal of both pluripotent human embryonic stem cells and induced pluripotent stem cells in a chemically defined, feeder - free and animal - protein free cell culture system.

With this information, they turned to a cell culture system to see if any Cpf1 nucleases would exhibit in vivo activity in mammalian cells.

The immortalized endothelial cell lines established from H - 2Kb - tsA58 mice provide, for the first time, a cell culture system to examine factors regulating angiogenesis and tumor cell arrest in different organ systems.

The researchers then used a 3 - D cell culture system to coax their mutant and healthy hPSCs down the differentiation pathway into becoming kidney progenitor cells and finally the proximal tubule cells found in kidney nephrons.

Glutamine addiction has often been studied in cell culture systems that are genetically altered to overproduce c - MYC, a central regulator of growth and proliferation in all cells that is frequently de-regulated in cancers.

In the cell culture systems, c - MYC protein will always be at a high level.

«The fact that glutamine addiction has mainly been investigated in cell culture systems may have overestimated the lethality of glutamine deprivation,» said Martin Eilers, University of Würzburg, who led the study together with Kempa.

As a result, 3D cell culture systems have become a staple of cell biology.

Cell culture systems used today also do not faithfully represent the features of human neurons.»

We thank Dominic Fan and Ewa Kruzel for sharing their expertise in cell culture systems, Walter Pagel for critical editorial review, and Lola López for expert assistance in the preparation of this manuscript.

«It is difficult to know whether the anti-amyloidogenic effect of resveratrol observed in cell culture systems can support the beneficial effect of specific diets,» Marambaud explains.

Many life - saving products, such as recombinant therapeutic proteins, vaccines, and gene therapy vectors, are produced from large - scale cell culture systems.

In the health care industry, many life - saving products, such as recombinant therapeutic proteins, vaccines, and gene therapy vectors, are produced from large - scale cell culture systems.

The technology also improves cell culture systems used to produce therapeutic proteins, vaccines, and gene therapy vectors.

Therapeutic applications of CRISPR - Cas gene editing are being advanced with the goals of finding cures and treatments for a range of serious diseases, developing needed medicines for both household pets and production animals, and improving cell culture systems for bioproduction.

Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission.

Bystander effects have been described in numerous in vitro cell culture systems.

Specifically, the Ignatius laboratory will study tumorigenic cell populations that self - renew and metastasize using a combination of zebrafish, murine xenograft and human cell culture systems.

From numerous studies in the Xenopus paired oocyte system and various cell culture systems, a reasonable assumption might be that if two cells express the same connexin or innexin and lie in close membrane apposition, gap junctions will probably be established between them; this assumption has not been investigated rigorously in vivo.

Antioxidants have been shown to reduce bio-markers of oxidative cell damage in cell culture systems, as well as in many animal models and human studies.

Flavonoids have been shown to reduce biomarkers of oxidative cell damage in cell culture systems, animal models and human studies.

Not exact matches

Heretofore, this earth has witnessed the emergence of single - celled living organisms, the growth of multicelled plant organisms, the advent of animals with centralized nervous systems making self - directed activity possible, and the flowering of humanity with its far - flung culture.

Another ingredient in this skin serum is ZPRO ™, which is the ZO Protein and Antioxidant System, made of butterfly bush meristem cell culture and hydrolyzed sericin.

The trophoblasts along with blood vessel cells were added to small dextran beads that were then spun around in a container filled with cell culture fluid, creating shear stress and rotational forces to better mimic the environment at the maternal - fetal interface than static cell - culture systems.

New developments include reaction systems for catalysis research, sampling from reactors, systems for high - throughput applications in synthesis, the handling of microorganisms or cell - culturing procedures, and more.

Although such harvested cells could be cultured as say, liver cells for treating hepatitis or dopamine - producing cells for Parkinson's, the resulting transplants would likely be rejected by patients» immune systems.

An interdisciplinary scientist is needed to demonstrate how the mechanical properties of the culture system alter signaling in the cells.

However, this coupling does not exist in cell culture models with exogenously expressed c - MYC — the type of systems that are mainly used to investigate glutamine addiction in cancer.

«As a biologist, I needed simple yet tunable cell - culture gels that would give me a defined system to study metabolites in the laboratory.

In a typical 3D culture system, cells cling to each other, forming spheroids that float in liquid culture media or expand into a soft matrix.

2 - D cell - culture and mouse experiments also provided key evidence of the virus's modus operandi; although the rodent brain doesn't harbor the full contingent of human neural stem cells, it has blood vessels and immune - system components that organoids lack.

«This pilot study showed that the culture system not only allowed for efficient propagation of sperm stem cells, but also eliminated ALL cells,» said Sadri - Ardekani.

They found that indeed, they do, and that stimulating these cells led them to kill cells infected with HIV - 1 derived from latently infected cells, both in culture and in mice engineered to have a human immune system.

protected animals»); studies on in vitro systems (whole perfused organs, tissue slices, cell and tissue cultures, and subcellular fractions); and human studies (including estimations of occupational and environmental exposure, postmarketing surveillance, epidemiology, and the ethical and strictly controlled use of human volunteers).

Our system runs in a normal cell culture dish, and there are no pumps; we use pressure heads to perfuse media through the vasculature.

To carry out the study, the team has analysed how different carbohydrates act on the surface of silver nanoparticles (Ag - NP) of around 50 nanometres, which have been introduced into cultures of liver cells and tumour cells from the nervous system of mice.

We have been using cell culture models and zebrafish as an assay to determine the effects of BMP on signaling, but we also want to be able to understand what the effect of the mutation is in the tissue and organ systems.

They tested the system on cell cultures, and then in live mice with breast cancer and mice with constricted arteries in their legs.

The scientists then tried the proline / polyproline combination in a test system of adherent cell cultures that were subjected to freeze - thaw conditions.

The proposed manipulation system makes it possible to place cells in a microwell array and observe the adherence, spreading, culture, and death of the cells.

Using her new culture system, she joined forces with colleagues to research which cells in an embryo contribute to which parts of the adult animal, a process called fate - mapping.

«But the unique developmental potential of a zygote, formed right after the sperm and egg meet, is very, very difficult to study, due to limited materials and the lack of a cell - culture experimental system.»

«Studies on embryonic development greatly benefit from the culture system of embryonic stem cells and, more recently, induced pluripotent stem cells.