Sentences with phrase «cell culture systems used»

The technology also improves cell culture systems used to produce therapeutic proteins, vaccines, and gene therapy vectors.

Cell culture systems used today also do not faithfully represent the features of human neurons.»

Not exact matches

However, this coupling does not exist in cell culture models with exogenously expressed c - MYC — the type of systems that are mainly used to investigate glutamine addiction in cancer.

The researchers then used a 3 - D cell culture system to coax their mutant and healthy hPSCs down the differentiation pathway into becoming kidney progenitor cells and finally the proximal tubule cells found in kidney nephrons.

protected animals»); studies on in vitro systems (whole perfused organs, tissue slices, cell and tissue cultures, and subcellular fractions); and human studies (including estimations of occupational and environmental exposure, postmarketing surveillance, epidemiology, and the ethical and strictly controlled use of human volunteers).

Our system runs in a normal cell culture dish, and there are no pumps; we use pressure heads to perfuse media through the vasculature.

We have been using cell culture models and zebrafish as an assay to determine the effects of BMP on signaling, but we also want to be able to understand what the effect of the mutation is in the tissue and organ systems.

Using her new culture system, she joined forces with colleagues to research which cells in an embryo contribute to which parts of the adult animal, a process called fate - mapping.

To investigate how the liver responds to bile accumulation, the research team used an artificial culture system, which allowed the easy manipulation of cultured liver cells.

«An important, overarching goal of the field is to develop culture systems using primary cells with minimal ex vivo [external] manipulation.

In recent years cultured Drosophila S2 cells have been used extensively as a model system to study infection and immune responses.

UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.

They use a wide range of tools and systems, including genetics in model organisms, cell culture, biochemistry, proteomics, and genomics.

Using the DEF ‑ CS cell culture component of the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System, we achieved an unparalleled survival rate of 6cell culture component of the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System, we achieved an unparalleled survival rate of 6Cell Cloning System, we achieved an unparalleled survival rate of 67 %.

Cells were cultured under non-differentiating conditions using the Cellartis DEF ‑ CS 500 Culture System.

Before and after using this system, hiPS cells are grown in the DEF ‑ CS culture system, which maintains cells as a karyotypically stable and pluripotent monolayer.

Taken together, these data indicate that hiPS cells expanded in the DEF ‑ CS culture system can be successfully edited using gesicles while retaining their pluripotency.

hiPS cells can be cultured, edited, and clonally expanded using the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System.

First, cells from Cellartis Human iPS Cell Line 22 (ChiPSC22) were modified to stably express AcGFP1, then these cells were cultured using the DEF ‑ CS culture system in a 48 - well plate.

Our lab uses a variety of model systems, such as the nematode C. elegans, as well as in vitro culture systems, to address fundamental questions in cell cycle control during development and tissue formation.

For cell therapy, automation can be used to increase the scale of cell culture operations (e.g., bioreactors replacing flasks) and allow the use of closed systems that can protect cell products from contamination with adventitious agents from the environment or operators themselves.

This protocol is used for general maintenance and passaging of hES and iPS cells in a feeder - independent culture system such as mTeSR1 / Matrigel.

To identify the JSRV receptor in lung cells, Miller developed a system using cultured hamster cells that contain small pieces of human DNA.

The «heart - on - a-chip,» which builds off previous successful iCHIP research on the peripheral and central nervous systems, involves the use of human cardiac cells cultured for up to nine days on the engineered chip.

Enriched by the rapid evolution of these technologies, TEFOR - TACGene has developed a solid expertise in the design, production and use of TALE - N and CRISPR / Cas9 systems, both for its own research projects in cultured cells and in collaboration, in many model organisms, including the rat, zebrafish, Drosophila, and Xenopus as part of the National Infrastructure in Biology and Health TEFOR supported by Investissement d'Avenir programme (2012 - 2019).

Electroporation of cultured cells was carried out using the Neon Transfection System (Invitrogen).

His efforts have pioneered the use of the three - dimensional «artificial skin» cultures to study the behavior of both tumor and normal cells that sustain tumor growth, a system known as the tumor microenvironment.

Specifically, the Ignatius laboratory will study tumorigenic cell populations that self - renew and metastasize using a combination of zebrafish, murine xenograft and human cell culture systems.

UNISI will provide expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.

For postmitotic neurons, we chose to use an established neuronal differentiation culture system in which NSCs were induced to differentiate into dopaminergic neurons by medium conditioned on stromal cells for 4 weeks.

Cortical development and cortical stem cells The laboratory uses neural stem cell culture, transgenic model systems and retroviral vectors to study signalling pathways that are central in mouse cortical development and in particular in cortical stem cells.

This study used a model cell culture system that represents what happens in an animal or human.

In addition, cats that have been vaccinated with some types of vaccines may test falsely positive on coronavirus antibody tests due to cross-reaction between components of the cell culture used to produce the vaccine and test system components.»

ACT - activated clotting time (bleeding disorders) ACTH - adrenocorticotropic hormone (adrenal gland function) Ag - antigen test for proteins specific to a disease causing organism or virus Alb - albumin (liver, kidney and intestinal disorders) Alk - Phos, ALP alkaline phosphatase (liver and adrenal disorders) Allergy Testing intradermal or blood antibody test for allergen hypersensitivity ALT - alanine aminotransferase (liver disorder) Amyl - amylase enzyme — non specific (pancreatitis) ANA - antinuclear antibody (systemic lupus erythematosus) Anaplasmosis Anaplasma spp. (tick - borne rickettsial disease) APTT - activated partial thromboplastin time (blood clotting ability) AST - aspartate aminotransferase (muscle and liver disorders) Band band cell — type of white blood cell Baso basophil — type of white blood cell Bile Acids digestive acids produced in the liver and stored in the gall bladder (liver function) Bili bilirubin (bile pigment responsible for jaundice from liver disease or RBC destruction) BP - blood pressure measurement BUN - blood urea nitrogen (kidney and liver function) Bx biopsy C & S aerobic / anaerobic bacterial culture and antibiotic sensitivity test (infection, drug selection) Ca +2 calcium ion — unbound calcium (parathyroid gland function) CBC - complete blood count (all circulating cells) Chol cholesterol (liver, thyroid disorders) CK, CPK creatine [phospho] kinase (muscle disease, heart disease) Cl - chloride ion — unbound chloride (hydration, blood pH) CO2 - carbon dioxide (blood pH) Contrast Radiograph x-ray image using injected radiopaque contrast media Cortisol hormone produced by the adrenal glands (adrenal gland function) Coomb's anti- red blood cell antibody test (immune - mediated hemolytic anemia) Crea creatinine (kidney function) CRT - capillary refill time (blood pressure, tissue perfusion) DTM - dermatophyte test medium (ringworm — dermatophytosis) EEG - electroencephalogram (brain function, epilepsy) Ehrlichia Ehrlichia spp. (tick - borne rickettsial disease) EKG, ECG - electrok [c] ardiogram (electrical heart activity, heart arryhthmia) Eos eosinophil — type of white blood cell Fecal, flotation, direct intestinal parasite exam FeLV Feline Leukemia Virus test FIA Feline Infectious Anemia: aka Feline Hemotrophic Mycoplasma, Haemobartonella felis test FIV Feline Immunodeficiency Virus test Fluorescein Stain fluorescein stain uptake of cornea (corneal ulceration) fT4, fT4ed, freeT4ed thyroxine hormone unbound by protein measured by equilibrium dialysis (thyroid function) GGT gamma - glutamyltranferase (liver disorders) Glob globulin (liver, immune system) Glu blood or urine glucose (diabetes mellitus) Gran granulocytes — subgroup of white blood cells Hb, Hgb hemoglobin — iron rich protein bound to red blood cells that carries oxygen (anemia, red cell mass) HCO3 - bicarbonate ion (blood pH) HCT, PCV, MHCT hematocrit, packed - cell volume, microhematocrit (hemoconcentration, dehydration, anemia) K + potassium ion — unbound potassium (kidney disorders, adrenal gland disorders) Lipa lipase enzyme — non specific (pancreatitis) LYME Borrelia spp. (tick - borne rickettsial disease) Lymph lymphocyte — type of white blood cell MCHC mean corpuscular hemoglobin concentration (anemia, iron deficiency) MCV mean corpuscular volume — average red cell size (anemia, iron deficiency) Mg +2 magnesium ion — unbound magnesium (diabetes, parathyroid function, malnutrition) MHCT, HCT, PCV microhematocrit, hematocrit, packed - cell volume (hemoconcentration, dehydration, anemia) MIC minimum inhibitory concentration — part of the C&S that determines antimicrobial selection Mono monocyte — type of white blood cell MRI magnetic resonance imaging (advanced tissue imaging) Na + sodium ion — unbound sodium (dehydration, adrenal gland disease) nRBC nucleated red blood cell — immature red blood cell (bone marrow damage, lead toxicity) PCV, HCT, MHCT packed - cell volume, hematocrit, microhematocrit (hemoconcentration, dehydration, anemia) PE physical examination pH urine pH (urinary tract infection, urolithiasis) Phos phosphorus (kidney disorders, ketoacidosis, parathyroid function) PLI pancreatic lipase immunoreactivity (pancreatitis) PLT platelet — cells involved in clotting (bleeding disorders) PT prothrombin time (bleeding disorders) PTH parathyroid hormone, parathormone (parathyroid function) Radiograph x-ray image RBC red blood cell count (anemia) REL Rocky Mountain Spotted Fever / Ehrlichia / Lyme combination test Retic reticulocyte — immature red blood cell (regenerative vs. non-regenerative anemia) RMSF Rocky Mountain Spotted Fever SAP serum alkaline phosphatase (liver disorders) Schirmer Tear Test tear production test (keratoconjunctivitis sicca — dry eye,) Seg segmented neutrophil — type of white blood cell USG Urine specific gravity (urine concentration, kidney function) spec cPL specific canine pancreatic lipase (pancreatitis)-- replaces the PLI test spec fPL specific feline pancreatic lipase (pancreatitis)-- replaces the PLI test T4 thyroxine hormone — total (thyroid gland function) TLI trypsin - like immunoreactivity (exocrine pancreatic insufficiency) TP total protein (hydration, liver disorders) TPR temperature / pulse / respirations (physical exam vital signs) Trig triglycerides (fat metabolism, liver disorders) TSH thyroid stimulating hormone (thyroid gland function) UA urinalysis (kidney function, urinary tract infection, diabetes) Urine Cortisol - Crea Ratio urine cortisol - creatine ratio (screening test for adrenal gland disease) Urine Protein - Crea Ratio urine protein - creatinine ratio (kidney disorders) VWF VonWillebrands factor (bleeding disorder) WBC white blood cell count (infection, inflammation, bone marrow suppression)

Execution of DNA extraction using the Promega genomic DNA purification system on cultured cell samples.