Sentences with phrase «cell culture techniques for»

Using a labor - intensive cell culturing technique for hunting retroviruses that he had pioneered in the 1970s, Ruscetti had transmitted the pathogen from patients» T - cells to uninfected T - cells in the laboratory.

Not exact matches

If a breast cancer line had silently contaminated Klonisch's cell culture early on, it would have been subject to the usual technique for immortalizing a normal cell (which involves applying enzymes, antibiotics and antibiotic - resistant genes).

Using a technique that introduces tiny wrinkles into sheets of graphene, researchers from Brown University have developed new textured surfaces for culturing cells in the lab that better mimic the complex surroundings in which cells grow in the body.

Brown University engineers have demonstrated a technique for making 3 - D - printed biomaterials that can degrade on demand, which can be useful in making intricately patterned microfluidic devices or in making cell cultures than can change dynamically during experiments.

Virus isolation is usually achieved using in vitro cell culture techniques, but this is not easily achieved from CCD bees because many are infected by multiple viruses and plaque purification techniques are not available for most of these viruses.

In the current study, lead authors David V. Hansen, PhD, a postdoctoral fellow, and Jan H. Lui, a graduate student in the Kriegstein lab, examined the OSVZ, using new labeling and tracking techniques to follow individual cells and their progeny over time in cultured tissue slices from fetal cortex tissue that had been donated for research.

«Our experimental model represents a promising technique to culture human liver cells and prepare them for transplantation on a biodegradable polymer scaffold into the peritoneal cavity,» concluded Dr. Pollok.

Once we can culture primordial germ cells and optimize germ - line transmission for Domestic Rock Pigeons, we can transfer those techniques to the Band - tailed Pigeon.

Ultimately, we use all this information to establish innovative culture techniques for generating unlimited neural stem cell sources for the derivation of specific types of clinically relevant neuronal or glial cells, for their use in disease modelling and drug discovery platforms, and towards refining their efficacy for future cell replacement - based therapy.

Advanced reproductive techniques such as artificial insemination, culturing of primordial germ cells for germ - line transmission, stem cell embryogenesis, and cloning offer an array of new ways to expand and even infuse lost and new genetic variability into at - risk wildlife populations.

Therefore, we have made second - generation feeder cells that stably express the canine form of CD40L (we previously used the human CD40L molecule in our feeder cells) and have evaluated a non cell - based technique for canine B cell culture.

Professional Duties & Responsibilities Biomedical and biotechnology engineer with background in design of biomaterials, biosensors, drug delivery devices, microfrabrication, and tissue engineering Working knowledge of direct cell writing and rapid prototyping Experience fabricating nanocomposite hydrogel scaffolds Proficient in material analysis, mechanical, biochemical, and morphological testing of synthetic and biological materials Extensive experience in bio-imaging processes and procedures Specialized in mammalian, microbial, and viral cell culture Working knowledge of lab techniques and instruments including electrophoresis, chromatography, microscopy, spectroscopy, PCR, Flow cytometery, protein assay, DNA isolation techniques, polymer synthesis and characterization, and synthetic fiber production Developed strong knowledge of FDA, GLP, GMP, GCP, and GDP regulatory requirements Created biocompatible photocurable hydrogels for cell immobilization Formulated cell friendly prepolymer formulation Performed surface modification of nano - particle fillers to enhance their biocompatibility Evaluated cell and biomaterial interaction, cell growth, and proliferation Designed bench - top experiments and protocols to simulate in vivo situations Designed hydrogel based microfluidic prototypes for cell entrapment and cell culture utilizing computer - aided robotic dispenser Determined various mechanical, morphological, and transport properties of photocured hydrogels using Instron, FTIR, EDX, X-ray diffraction, DSC, TGA, and DMA Assessed biocompatibility of hydrogels and physiology of entrapped cells Evaluated intracellular and extracellular reactions of entrapped cells on spatial and temporal scales using optical, confocal, fluorescence, atomic force, and scanning electron microscopies Designed various biochemical assays Developed thermosensitive PET membranes for transdermal drug delivery application using Gamma radiation induced graft co-polymerization of N - isopropyl acylamide and Acrylic acid Characterized grafted co-polymer using various polymer characterization techniques Manipulated lower critical solution temperature of grafted thermosensitive co-polymer Loaded antibiotic on grafted co-polymer and determined drug release profile with temperature Determined biomechanical and biochemical properties of biological gels isolated from marine organisms Analyzed morphological and mechanical properties of metal coated yarns using SEM and Instron Performed analytical work on pharmaceutical formulations using gas and high performance liquid chromatography Performed market research and analysis for medical textile company Developed and implement comprehensive marketing and sales campaign