Sentences with phrase «cell culturing technique»

Using a labor - intensive cell culturing technique for hunting retroviruses that he had pioneered in the 1970s, Ruscetti had transmitted the pathogen from patients» T - cells to uninfected T - cells in the laboratory.

The research group studied the role of oxidative stress (see «The Two Faces of Oxygen») in cell senescence, and at Packer's request, Smith began teaching Pereira - Smith cell culture techniques.

Virus isolation is usually achieved using in vitro cell culture techniques, but this is not easily achieved from CCD bees because many are infected by multiple viruses and plaque purification techniques are not available for most of these viruses.

Techniques such as flow cytometry, genomics and multiple cell culture techniques can help determine T - cells reactivity to antigen as well as help find unknown antigens.

In cases of neonatal mortality, the diagnosis typically is made postmortem with virus isolation from fresh lung, liver, kidney, and spleen by cell culture techniques and subsequent identification by PCR and sequencing, transmission electron microscopy, immunofluorescence, or fluorescence in situ hybridization.

Not exact matches

She was always ready to learn new techniques, and she diversified her skill set by working in the animal facility, cell culture room, and also in a mass spectrometry lab.

The team tested the technique on cells in culture that mimic the transformation of unspecialised white blood cells into full immune cells.

If a breast cancer line had silently contaminated Klonisch's cell culture early on, it would have been subject to the usual technique for immortalizing a normal cell (which involves applying enzymes, antibiotics and antibiotic - resistant genes).

When the researchers used gene engineering techniques to knock out DDX3 expression in laboratory - grown cell cultures that highly expressed this protein, cell proliferation was half that of cell cultures with high DDX3 expression.

Recently, the Lerner laboratory developed an advanced technique in which hundreds of millions of distinct antibodies are produced artificially within very large cultures of mammalian cells.

He improved the technique by culturing the stem cells with astrocytes, cells that guide the maturing neurons, that he had taken from the dopamine - producing region of the rat brain.

A major challenge in creating any type of organoid is determining the unique mixture of nutrients, growth factors, and tissue culture techniques that will transform patient tumor cells into miniature tumor organoids in a petri dish.

«Until now, retention of salivary gland cell properties has not been possible using other tissue culture techniques.

Using a technique that introduces tiny wrinkles into sheets of graphene, researchers from Brown University have developed new textured surfaces for culturing cells in the lab that better mimic the complex surroundings in which cells grow in the body.

Brown University engineers have demonstrated a technique for making 3 - D - printed biomaterials that can degrade on demand, which can be useful in making intricately patterned microfluidic devices or in making cell cultures than can change dynamically during experiments.

Before toying with worms, the S.U.N.Y. Buffalo team tried out their technique on cultures of human embryonic kidney cells and neurons from a rat hippocampus, a part of the brain integral to memory.

Balaban agrees that the technique must be tested in animals to gauge its effectiveness in the body (as opposed to the less complex environment of a cell culture).

They used a technique pioneered at Smith's lab that uses cultures of various inhibitory proteins to keep embryonic stem cells continually renewing themselves rather than differentiating into other cells.

They diluted the microbial sample such that they could add a single cell per culture well filled with their new media via a technique called dilution - to - extinction.

The research builds on the team's previous work with a technique called three - dimensional culture, which involves incubating stem cells in a floating ball - shaped aggregate, unlike traditional cell culture in which cells grow in a flat layer on the surface of a culture dish.

To test the quality of the «blood vessels» produced, the researchers cultured a chunk of tissue made from rat liver cells using their technique.

We worked with animal cell cultures and molecular biology techniques (and the occasional sheep if we were developing veterinary applications).

To show that the Cal - Light technique is effective, Dr. Kwon's group tested it first in cell culture, and then in vivo in a mouse model.

Specific duties will include basic and advanced polymer chemistry protocols, cell culture and expansion, hydrogel biomaterial characterization, physical characterization of biomaterials, assembly of 3D constructs using biofabrication and bioprinting techniques, collection and organization of data, and assisting in the preparation of reports and manuscripts.

In addition, the molecular beacons technique http://www.lependart.com could have broad applications across regenerative medicine, because it could be used with other types of cells produced from stem cell cultures, such as brain cells or insulin - producing islet cells.

Besides learning many cell biology techniques (tissue culture, Westerns, generating DNA constructs, transfections) as well as fly genetics, students can get familiar with imaging techniques depending on their own interests and experience.

In the current study, lead authors David V. Hansen, PhD, a postdoctoral fellow, and Jan H. Lui, a graduate student in the Kriegstein lab, examined the OSVZ, using new labeling and tracking techniques to follow individual cells and their progeny over time in cultured tissue slices from fetal cortex tissue that had been donated for research.

«Our experimental model represents a promising technique to culture human liver cells and prepare them for transplantation on a biodegradable polymer scaffold into the peritoneal cavity,» concluded Dr. Pollok.

The Cravatt laboratory soon developed powerful inhibitors of the newly discovered enzymes, and the two labs began working together, using the inhibitors and genetic techniques to explore the enzymes» functions in vitro and in cultured cells.

This unique workshop provides the introduction and practical hands - on of integrated metabolomics workflows, employing the techniques of cellular energy metabolism (Agilent Seahorse XF), detailed metabolomic analysis by LC / MS Q - TOF and the translation of cell culture results into a living mouse model using indirect calorimetry.

Finally, we have developed new techniques utilizing surface sensing of translation (SUnSET) and bioorthogonal noncanonical amino acid tagging (BONCAT) coupled with stable isotope labeling by amino acid in cell culture (SILAC) to selectively isolate and quantify newly synthesized proteins within specific temporal windows.

Develop KI techniques using modified oligonucleotides in cultured cells and in model organisms

Expertise in in - vitro culture of respiratory cell lines and primary human cells, as well as knowledge of basic molecular biology concepts and techniques is a must.

Once we can culture primordial germ cells and optimize germ - line transmission for Domestic Rock Pigeons, we can transfer those techniques to the Band - tailed Pigeon.

In addition, pluripotent stem cells tend to be genetically unstable, particularly if less than optimal culture techniques are used.

Ultimately, we use all this information to establish innovative culture techniques for generating unlimited neural stem cell sources for the derivation of specific types of clinically relevant neuronal or glial cells, for their use in disease modelling and drug discovery platforms, and towards refining their efficacy for future cell replacement - based therapy.

The center will develop techniques to test the drugs in cell culture and animal models, to validate their effects on a certain disease.

Advanced reproductive techniques such as artificial insemination, culturing of primordial germ cells for germ - line transmission, stem cell embryogenesis, and cloning offer an array of new ways to expand and even infuse lost and new genetic variability into at - risk wildlife populations.

Dr. Hackam's team has developed techniques of intestinal stem cell isolation and culture, and are working with tissue engineers and chemists towards the development of an artificial intestine, which has been tested in large and small pre-clinical models.

Behavior of the two novel mucins will be examined in cultured TM cells and in the TM of intact eyes using recombinant DNA, biochemical, and imaging techniques in order to provide clues to function.

The projects will provide training in: current molecular biology techniques (including RNA interference, genetic transformation, analyses of gene expression); cell biology techniques (cell culturing, cell transfections, imaging); protein biochemistry (2D electrophoresis, protein functional analyses); and bioinformatics (structure predictions, phylogenetic analyses; molecular interactions).

The projects are multidisciplinary, involving a variety of techniques including molecular biology (PCR, cloning), cell culture and advanced fluorescence microscopy.

Our approaches include gene discovery, mapping of signaling networks in cell culture models and in vivo functional analyses done through gene knock - out and knock - in techniques in mice.

My research made use of a range of cell culture, cell imaging, molecular and bioinformatic techniques, to investigate the evolution of hydrogenosomes (mitochondrial homologues that make hydrogen) in some anaerobic protists, found in a variety of naturally anoxic habitats.

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It includes a new, streamlined chapter that combines the review of alternative techniques to animal experimentation such as the use of isolated organs, cell cultures, and computer simulations.

In the 2000s, most AIDS research used cell cultures, fetal kittens, and molecular techniques to elucidate the virus» structure and function.

Therefore, we have made second - generation feeder cells that stably express the canine form of CD40L (we previously used the human CD40L molecule in our feeder cells) and have evaluated a non cell - based technique for canine B cell culture.

Microscopy Tissue Culture Multi-Color Flow Cytometry Assay Development High - Content Imaging Protein Expression & Purification Techniques Molecular Genetics Techniques Western Blot ELISA Immunofluorescence Microscopy Human Primary Cell Culture Data Analysis

Motivated professional research assistant with experience in cell culture, data management, and analytical laboratory techniques.