Samples were prepared for
cell cycle analysis and immunoblotting.
The cell cycle analysis was done using FACS Diva Software (BD Biosciences)[14].
Sections from all cases were subjected to stain with haematoxylin and eosin (H&E) for histopathological examination and immunohistochemical technique for Cyclin D1 in addition using flow cytometric technique to perform
cell cycle analysis.
This approach could also be used in many different contexts, not only for
cell cycle analysis,» adds Prof. Dr. Dr. Fabian Theis, head of the ICB.
Not exact matches
The
analysis of the ploidy of minichromosomes in
cells that are released from arrest demonstrates that the sister molecules are properly segregated when the
cell cycle block is removed.
Researchers at the University of Edinburgh and the MRC Laboratory for Molecular Biology in Cambridge used molecular
analysis to find that concentrations of magnesium rose and fell in a 24 - hour
cycle in all
cell types, and that this impacts on the
cells» internal clocks.
After a more detailed
analysis, however, the team found a minideletion affecting only two genes, UFD1 and a
cell cycle control gene called CDC45.
«This [concept] has been previously described in other biological processes, such as circadian rhythmicity, the
cell cycle, and even stem
cell pluripotency, but is shown here through a systematic
analysis of a data - driven computational model.»
The Institute is currently running on a Site License by Flowjo, a flow cytometry data
analysis software package which offers a range of
analysis platforms that includes compensation,
cell cycle, proliferation studies and kinetics.
Ralf Kittler (Buchholz, MPG)-- «Functional genomic
analysis of
cell cycle progression in human tissue culture
cells» (2006)
Panel D. mRNA expression
analysis of urea
cycle enzymes, as quantified by transcriptome
analysis, was performed in enhanced hiPS - HEP
cells from C12, C18, and C22 on Day 13 post-thawing (n = 2 batches per
cell line) and compared to hphep
cells on Day 1 post-thawing (n = 3 donors).
(b) The same flow cytometry
analysis for BCM2 (total
cell population) and BCM2 F3 (
cycling hypoxia - selected subpopulation)
cells.
(B) FACs
analysis of the
cell cycle in proliferating hNSCs, hESCs, and iPS
cells with a chart showing percentages of
cell types in the G2 / M phase of the
cell cycle.
Researchers at SciLifeLab / Uppsala University headed by Johan Elf have used a combination of microfluidics and image
analysis of E. coli with fluorescent labels on the replication machinery to study how the replication and division
cycles are coupled in individual
cells.
Other flow cytometry applications include: cellular phenotyping by multiparameter cytofluorimetric
analysis, sorting of fluorescence protein - tagged
cells, assessing apoptosis and
cell cycle status, the identification / isolation of stem and cancer stem
cells (e.g., marker positive, side - population, etc.) and the deposition of single
cells.
Identification of
cell cycle - related regulatory motifs using a kernel canonical correlation
analysis
The
analysis, published in the ACS journal Environmental Science & Technology, addressed both fuel
cycle and vehicle manufacturing
cycle for the following vehicle types: gasoline and diesel internal combustion engine vehicles (ICEVs); flex fuel vehicles; compressed natural gas (CNG) vehicles; hybrid electric vehicles (HEVs); hydrogen fuel
cell electric vehicles (FCEVs); battery electric vehicles (BEVs); and plug - in hybrid electric vehicles (PHEVs).