Sentences with phrase «cell cycle phase»

So far fluorescent stains have been used to assign cells to their cell cycle phase.

Thus, the initiation and completion of each cell cycle phase has numerous checks and balances to ensure that DNA replication and chromosome separation occur properly.

Not exact matches

This maneuver «froze» the cells in a quiescent phase of their division cycle and may have made their chromosomes more susceptible to being reprogrammed to initiate the growth of a new organism after the nuclei were transferred into an egg.

There are as many as 14, but the scientists found that cyclin D adds just a single phosphate at one, and only one, of the 14 locations during the early G1 phase of cell cycle progression, essentially make 14 different versions of the Rb tumor suppressor.

They further show that this diterpine downregulates HMG CoA reductase offering support to the concept that mevalonate deprivation causes cell cycle arrest at the G1 phase leading to apoptotic death of the prostate carcinoma cells.

Researchers at Texas Woman's University have shown that a diterpene geranylgeraniol found in linseed oil, Cedrela toona wood oil, sucupira branca fruit oil and more recently, annatto seed oil, suppressed the viability of human DU145 prostate carcinoma cells via cell cycle arrest at the G1 phase and the initiation of apoptosis.

First, imaging experiments revealed that Tregs have a close relationship with the stem cells that reside within hair follicles and allow them to regenerate: the number of active Tregs clustering around follicle stem cells typically swells by three-fold as follicles enter the growth phase of their regular cycle of rest and regeneration.

The study, which can be read in Nature Communications, shows the transcription factor family MYB3R prevents progression to the division stage (M phase) of the cell cycle in Arabidopsis, a small flowering plant that is a member of the mustard family.

Prof. Umeda found that inhibiting CDKs even with the absence of DNA damage would cause the Rep - MYB3R accumulation seen with DNA damage and stall the cell cycle before the M phase transition.

These phases (cell cycle) are repeated leading to cell division.

In order to figure out which phase that chem7 actually acts upon, Ueda and her team used two fluorescent proteins of different colors to visualize the process of the cell cycles in the root of Arabidopsis thaliana.

These are other proteins that are periodically formed and again broken down during the various phases of the cell cycle.

The researchers found that timing — specifically, the phase of the cell division cycle that bacteria were in when exposed to the antibiotic — had a significant effect on susceptibility.

Paull and Whikehart [7] have also shown that p53 and TAp63 (a p53 family member) are relatively elevated in the normal central human corneal endothelium suggesting that these proteins inhibit cell - cycle promotion from the G1 to S phase.

«Most adult mammalian beta cells are in a quiescent phase, and so if you want to push them into the cell cycle, you need to shake them out of their sleep,» explains Kulkarni, who is also a Professor of Medicine at Harvard Medical School.

ARandomized, Phase III, Double - Blind Placebo - Controlled Trial of Sunitinib as Maintenance Therapy in Non-Progressing Patients following an Initial Four Cycles of Platinum - Based Combination Chemotherapy in Advanced, Stage IIIB / IV Non-Small Cell Lung Cancer

It is currently suggested that HCECs are held in the G1 phase of the cell cycle and can not pass readily into the S phase prior to cell division [14].

Key findings of this research, which was conducted by Shawn L. Chavez, PhD and her colleagues at Stanford University and analyzed at OHSU, showed that by looking at the duration of the first mitotic phase — a short period in the cell cycle — one can identify chromosomally normal versus abnormal embryos up to approximately the 8 - cell stage.

In this new study, licensing refers to origin licensing, the process of helicase loading onto DNA for genome duplication purposes during the G1 phase of the cell cycle.

This acquisition can be divided into several interdependent and sequential phases, from the time point when progenitor cells exit the cell cycle toward the newly formed and perfectly differentiated neuron.

Moreover; the PI3K / AKT pathway also can contribute to melanoma tumorigenesis through mutations or loss in PTEN and dysregulation in expression of AKT, which positively regulates the G1 / S phase progression in cell cycle, suppresses apoptosis and promotes cellular survival.

Overgrown cultures stimulate quiescence, the resting phase of the cell cycle; quiescent cells do not take up nucleic acid or express transgenes as well as actively dividing cells resulting in lower lentiviral titers.

This cell cycle arrest in the G0 phase requires the retinoblastoma tumor suppressor protein (Rb).

The major mechanism of B cell suppression by MSCs is attributed partly to the physical contact between MSCs and B cells and in part to the soluble factors released by MSCs; this leads to the blocking of B cell proliferation in the G0 / G1 phase of the cell cycle with no apoptosis 28, 29, 30.

Cyclin D1 - levels varying throughout cell cycle, hence its expression in G1 phase increases in the cell that respond to enter a new cycle after receiving growth factor stimulation [8].

Cell cycle S - phase fraction was assessed by a colorimetric bromodeoxyuridine incorporation assay.

According to Wikipedia, old pores are disassembled and assembled during different phases of the cell cycle.

Cell cycle studies also showed that inhibition of mTOR function by using rapamycin or inhibition of mTOR expression by using mTOR - specific siRNA induced cell cycle arrest as shown by a dose - dependent decrease of cell cycle S phase (data not shoCell cycle studies also showed that inhibition of mTOR function by using rapamycin or inhibition of mTOR expression by using mTOR - specific siRNA induced cell cycle arrest as shown by a dose - dependent decrease of cell cycle S phase (data not shocell cycle arrest as shown by a dose - dependent decrease of cell cycle S phase (data not shocell cycle S phase (data not shown).

These results suggest that inhibition of mTOR - raptor complex or silencing mTOR gene expression induced cell cycle arrest at G1 phase in ALK + ALCL cells.

Instead, they discovered that each V. carteri cell type expresses orthologs of genes from different temporal phases of the light — dark cycle in a related unicellular alga, Chlamydomonas reinhardtii, with somatic cells enriched for expression of dark - phase genes, and gonidial cells enriched for expression of light - phase genes.

cell type expresses orthologs of genes from different temporal phases of the light — dark cycle in a related unicellular alga,

The researchers showed that i - motifs mostly form at a particular point in the cell's «life cycle» — the late G1 phase, when DNA is being actively «read».

Inhibition of mTOR expression or function blocked cell cycle progression from G1 to S phase associated with modulation of the CDK inhibitors p21waf1 and p27kip1.

Two newly synthesized 5 - methyltetrahydrofolate - like compounds inhibit methionine synthase activity accompanied by cell cycle arrest in G1 / S phase and apoptosis in vitro.

This contradictory behavior explained that Cyclin D1 acts as a negative regulative protein under some conditions rather than a positive regulator in the cell cycle, i.e. the modest increase in Cyclin D1 expression could hasten the G1 phase while the overexpression may lead to block the progression through the S - phase [28, 29].

In the correlation between the Cyclin D1 IOD and the different phases of the cell cycle it was noticed that, there was no any statistically association between Cyclin D1 - IOD and G1 phase % or S phase % in the all of individual groups.

This system is used to obtain the percentage of cells in each phase (G0 / G1, S and G2 / M) of the cell cycle for each sample.

While during S phase the cell automatically suppresses Cyclin D1 formation because it has the ability to inhibit DNA synthesis which may block the cycle progression and lead to the cell - arrest in this position [9].

(B) FACs analysis of the cell cycle in proliferating hNSCs, hESCs, and iPS cells with a chart showing percentages of cell types in the G2 / M phase of the cell cycle.

Human pluripotent stem cells and the human embryo: Human pluripotent stem cells (hPSC) have opened up a previously inaccessible phase of the human life cycle to...

The quantification showed that expanding cells do not enter this cell ‐ cycle phase once reaching a threshold cell size, or cell size increment or after a time interval.

Quantification of this data showed that hNSC - derived iPS cells were more similar to hESCs in the percentage of cells in the G2 / M phase of the cell cycle than were hNSCs (Figure S1b).

Instead, aneuploid NSCs present an extended G1 phase, which leads to cell cycle exit and premature differentiation.

ActD has been shown to have a reversible cytostatic effect and the ability to arrest cells in both the G1 and G2 phase of the cell cycle [17].

These three CPEBs have sequential non-redundant functions that promote the phase - specific polyadenylation and translational activation of CPE - regulated transcripts in the mitotic cell cycle.

This blocks promotion of the cell cycle from the G1 to the S phase.

Cyclins form complexes with cyclin - dependent kinases (CDKs), unique to each cell - cycle phase, and activate specific genes to drive cells through their cycles.

In the 1990s, researchers were beginning to understand the ways cells regulate transitions into the four phases of the cell cycle: G1, synthesis (S) phase, G2, and mitosis.

Finally, we show that inactivation of DNA damage checkpoint kinases in senescent cells can restore cell - cycle progression into S phase.

The degradation allows the cell to enter the next phase of the cycle.