In order to better understand how the scientists have come to this conclusion, one needs to get familiar with
cell differentiation markers.
The Intestinal Epithelial
Cell Differentiation Marker ALPi is Selectively Induced by HDAC Inhibitors in Colon Cancer Cells in a KLF5 - dependent Manner.
Not exact matches
The results showed that, when applied, a long - term inhibition of Rho - kinase signaling increased the expressions of chondrocyte - specific genes and
differentiation markers in human chondrosarcoma 2/8
cells.
Expanding from their previous studies with mice, the researchers first established that under specific conditions, culturing human embryonic stem
cells with fibroblast growth factor 2 (FGF2) leads to neural
differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of
markers for the cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.
A short - term inhibition of Rho - kinase failed to induce extracellular matrix production in fibroblasts or in HCS - 2 / 8
cells, while its long - term exposure increased the expressions of chondrocyte - specific genes and
differentiation markers, and also promoted the synthesis of sulfated glycosaminoglycans by chondrocytic
cells.
We will develop mathematical models to predict the pathways of
differentiation from naive to memory and effector T -
cell subsets based on the characterisation of surface
marker expression, transcription factors and cytokines production at early and late time points after immunisation.
After 15 days, a subset of
cells expressed regulators of RGC
differentiation (Atoh7, Brn3b, and Islet1), while a smaller subset co-expressed Brn3b and the mature RGC
marker Thy1, thus confirming a RGC identity.
Ihor Zahanich (Ravens, TUD)-- «
Differentiation potential of human mesenchymal stem
cells: expression of molecular
markers, ion channels and gap junction channels» (2006)
Pluripotent
marker expression and
differentiation of human second trimester mesenchymal stem
cells.
MyoD is normally only expressed in skeletal muscle, and it was later found to be a transcription factor involved in the
differentiation of muscle
cells and also a very early
marker of muscle
cell fate commitment.
Interestingly,
cells from both iPS
cell lines expressed early cardiac
differentiation markers and transcription factors, implicating that there could be a lack in functional cardiomyocyte maturation in line 4.1.
For definitive endoderm
differentiation, spontaneously formed EBs on day 6 were plated onto matrigel - coated dishes and maintained in a low concentration of fetal bovine serum (FBS) in combination with a high concentration of recombinant Activin A. Scale - like
cells appeared (Figure 5A), which were positive for definitive endoderm
markers such as Foxa2, Sox17 and Gata4.
The early cardiac
markers and transcription factors (Gata4, Mef2c, Hind I, Nkx2 - 5 and beta - Mhc) were examined in iPS
cell lines 11.1 and 4.1 during the cardiac
differentiation process (
differentiation day 0, day 3, day 6 and day 9).
«The observed increase in the ratio of cytotoxic CD4 T
cells to CD4 helper T
cells indicates that they are an important component of the protective immune response to viral infections and that their induction should be an important
marker for successful vaccinations against certain viral diseases,» says postdoctoral researcher and first author Veena Patil, Ph.D. «But we really didn't know enough about their molecular profile and the mechanisms that drive their
differentiation and maintenance.»
In addition, the expression of other germ
cell markers, such as NANOS and PIWIL1 genes, was also up - regulated as the EB
differentiation progressed.
Among the early germ
cell markers examined, VASA is a candidate gene for detecting pre-meiotic germ
cell differentiation from monkey ES
cells, because its expression is detected earlier in the primordial stage of germ
cell development in comparison to that of PIWI family genes in vivo in mice and humans [11], [36]--[38], [49].
However, these germ
cell marker genes are not appropriate for detecting germ
cell differentiation from mouse and human ES
cells because these genes are expressed in both ES
cells and germ
cells.
A combination of several germ
cell markers will thus be necessary to detect germ
cell differentiation from ES
cells.
To explore the
differentiation markers for detecting germ
cells differentiated from ES
cells, the expression of various germ
cell marker genes was examined in tissues and ES
cells of the cynomolgus monkey (Macaca fascicularis).
Here, ES
cells transduced with HOXB4 were analyzed for the expression of hematopoietic
markers after 26 days of
differentiation.
We have previously reported that our 26 - d protocol closely recapitulates key stages of biliary development, starting with the
differentiation of hPSCs into endoderm and subsequently into foregut progenitor (FP)
cells, followed by the generation of hepatoblasts (HBs), cholangiocyte progenitors (CPs) expressing early biliary
markers and mature CLCs displaying cholangiocyte functionality.
Here, we used a
marker - free approach to computationally reconstruct the blood lineage tree in zebrafish and order
cells along their
differentiation trajectory, based on their global transcriptional differences.
After 4 weeks of
differentiation, the majority of the
cells (> 60 %) expressed the postmitotic neuronal
marker β - III tubulin with a subset (about 50 % of total neurons) additionally expressing TH, a
marker for midbrain dopaminergic neurons (Fig. 3A — D).
Fluorescence - based assays Fluorescent
markers such as EGFP, YFP, mCherry and mTomato or fluorescent biosensors can be used to measure a variety of real - time
cell - based activities, including, intracellular transport, protein signaling, receptor desensitization, migration, division, apoptosis, metabolism,
differentiation, chemotaxis, transcription and translation.