Sentences with phrase «cell electrophoresis»

«Flow cytometry identified a large population of apoptotic melanoma cells, and single - cell electrophoresis indicated that mensacarcin causes genetic instability, a hallmark of early apoptosis,» Loesgen said.

Not exact matches

Patent protection can also be sought for a new way to culture cells (a «method») or for a novel electrophoresis apparatus (a «machine»).

Although the primary effect of the electric field is cracking cell structures for better availability of nutrition, other effects like shockwave, electrophoresis or influence on the metabolic condition of cells can play a role.

After a few days, we isolate the DNA from the cells, amplify it with the polymerase chain reaction, and use electrophoresis to show that the CD32 gene has been cut into pieces.

Two - dimensional polyacrylamide gel electrophoresis combined with chromatographic phosphoprotein pre-enrichment and electrospray ionization mass spectrometry - based protein identification was used to reveal changes of phosphoproteins in cells exposed to cyclic stretching.

We offer a comprehensive selection of PCR - based B - and T - cell clonality assays for gel and capillary electrophoresis detection, which also include BIOMED - 2 reagents for clonality and translocations, exclusively offered by Invivoscribe.

For some applications, a qPCR wil... Keywords: Amplification, Cancer, Capillary electrophoresis, Cell culture, Degradations, Detection methods, Electrophoresis, Gene expression, Growth factors, Indicators, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA purification, Sample preparationselectrophoresis, Cell culture, Degradations, Detection methods, Electrophoresis, Gene expression, Growth factors, Indicators, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA purification, Sample preparationsElectrophoresis, Gene expression, Growth factors, Indicators, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA purification, Sample preparations, Transcription

The gelatinolytic assays were carried out in two different ways to obtain complementary information: firstly, cells were treated or not with the test compound and samples from these were submitted to gelatinase zymography to detect the effects of the kahweol treatment on the expression of gelatinase activities; secondly, in some experiments, samples from control, untreated HT - 1080 fibrosarcoma cells were submitted to zymography and, after electrophoresis, different concentrations of kahweol were added to the substrate buffer to determine the potential direct effect of kahweol on gelatinase activity.

The projects will provide training in: current molecular biology techniques (including RNA interference, genetic transformation, analyses of gene expression); cell biology techniques (cell culturing, cell transfections, imaging); protein biochemistry (2D electrophoresis, protein functional analyses); and bioinformatics (structure predictions, phylogenetic analyses; molecular interactions).

Carrier screening for hemoglobinopathies such as sickle cell anemia is recommended by the American Congress of Obstetricians and Gynecologists (ACOG) via complete blood count and hemoglobin electrophoresis for people of African, Southeast Asian, Mediterranean, Middle Eastern, and West Indian descent considering having children.

In this high - tech lab, students can perform tests like gel electrophoresis, analyzing hemoglobin samples to determine whether a patient has sickle cell disease.

First, she will use the clinical method of gel electrophoresis (commonly used to visualize DNA) to conduct a «hormone race» between cells composed of different ratios of estrogen and testosterone.

Tags for this Online Resume: ELISA,, Electrophoresis (SDS - PAGE, Western Blotting), Chromatography (TLC, CE)-RRB--RRB-, Spectrophotometery (Bradford, A280, BCA)-RRB-, Cell culture, cell proliferation, RNA, DNA, Q PCR, Virology, Viral Safety Testing (in - vitro, retrovirus, reverse transcriptase assays, Electrophoresis (SDS - PAGE, Western blot), Chromatography (TLC, CE, HPLC), Spectrophotometry (Bradford, A280, Cell culture, cell proliferation, RNA, DNA, Q PCR, Virology, Viral Safety Testing (in - vitro, retrovirus, reverse transcriptase assays, Electrophoresis (SDS - PAGE, Western blot), Chromatography (TLC, CE, HPLC), Spectrophotometry (Bradford, A280, cell proliferation, RNA, DNA, Q PCR, Virology, Viral Safety Testing (in - vitro, retrovirus, reverse transcriptase assays, Electrophoresis (SDS - PAGE, Western blot), Chromatography (TLC, CE, HPLC), Spectrophotometry (Bradford, A280, BCA)

Tags for this Online Resume: Ecl (Control Lang), Purchasing, Software, ARIA, Injections, Intelligent Call Management, Research, Transcription, Genotyping, Western blot, viral vectors, DNA cloning, RNA extraction, RNA syntehesis, confocal microscopy, cell culture, transfection, transduction, western blot, PCR, RT - qPCR, Next Generation DNA sequencing, ion torrent, gromacs, ICM, Schrodinger Tools, NCBI software, DIVA, FlowJaw, Flow Cytometry, Cell Sorting, Aria II, Aria III, XCalibur, TaqMan, electrophoresis, Sanger DNA sequencing, FCS Express, cell patterning, ELISA, immunoassay, antibody, HPLC, his - tag, flag - tag, flash - tag, chromatography, stem cells, DNA extraction, CRISPR, cas9, plasmid, lentivirus, CAR - technology, MLV, Chimeric Antigen Receptors, Jurkat, U87, HeLa ccell culture, transfection, transduction, western blot, PCR, RT - qPCR, Next Generation DNA sequencing, ion torrent, gromacs, ICM, Schrodinger Tools, NCBI software, DIVA, FlowJaw, Flow Cytometry, Cell Sorting, Aria II, Aria III, XCalibur, TaqMan, electrophoresis, Sanger DNA sequencing, FCS Express, cell patterning, ELISA, immunoassay, antibody, HPLC, his - tag, flag - tag, flash - tag, chromatography, stem cells, DNA extraction, CRISPR, cas9, plasmid, lentivirus, CAR - technology, MLV, Chimeric Antigen Receptors, Jurkat, U87, HeLa cCell Sorting, Aria II, Aria III, XCalibur, TaqMan, electrophoresis, Sanger DNA sequencing, FCS Express, cell patterning, ELISA, immunoassay, antibody, HPLC, his - tag, flag - tag, flash - tag, chromatography, stem cells, DNA extraction, CRISPR, cas9, plasmid, lentivirus, CAR - technology, MLV, Chimeric Antigen Receptors, Jurkat, U87, HeLa ccell patterning, ELISA, immunoassay, antibody, HPLC, his - tag, flag - tag, flash - tag, chromatography, stem cells, DNA extraction, CRISPR, cas9, plasmid, lentivirus, CAR - technology, MLV, Chimeric Antigen Receptors, Jurkat, U87, HeLa cells

Professional Duties & Responsibilities Biomedical and biotechnology engineer with background in design of biomaterials, biosensors, drug delivery devices, microfrabrication, and tissue engineering Working knowledge of direct cell writing and rapid prototyping Experience fabricating nanocomposite hydrogel scaffolds Proficient in material analysis, mechanical, biochemical, and morphological testing of synthetic and biological materials Extensive experience in bio-imaging processes and procedures Specialized in mammalian, microbial, and viral cell culture Working knowledge of lab techniques and instruments including electrophoresis, chromatography, microscopy, spectroscopy, PCR, Flow cytometery, protein assay, DNA isolation techniques, polymer synthesis and characterization, and synthetic fiber production Developed strong knowledge of FDA, GLP, GMP, GCP, and GDP regulatory requirements Created biocompatible photocurable hydrogels for cell immobilization Formulated cell friendly prepolymer formulation Performed surface modification of nano - particle fillers to enhance their biocompatibility Evaluated cell and biomaterial interaction, cell growth, and proliferation Designed bench - top experiments and protocols to simulate in vivo situations Designed hydrogel based microfluidic prototypes for cell entrapment and cell culture utilizing computer - aided robotic dispenser Determined various mechanical, morphological, and transport properties of photocured hydrogels using Instron, FTIR, EDX, X-ray diffraction, DSC, TGA, and DMA Assessed biocompatibility of hydrogels and physiology of entrapped cells Evaluated intracellular and extracellular reactions of entrapped cells on spatial and temporal scales using optical, confocal, fluorescence, atomic force, and scanning electron microscopies Designed various biochemical assays Developed thermosensitive PET membranes for transdermal drug delivery application using Gamma radiation induced graft co-polymerization of N - isopropyl acylamide and Acrylic acid Characterized grafted co-polymer using various polymer characterization techniques Manipulated lower critical solution temperature of grafted thermosensitive co-polymer Loaded antibiotic on grafted co-polymer and determined drug release profile with temperature Determined biomechanical and biochemical properties of biological gels isolated from marine organisms Analyzed morphological and mechanical properties of metal coated yarns using SEM and Instron Performed analytical work on pharmaceutical formulations using gas and high performance liquid chromatography Performed market research and analysis for medical textile company Developed and implement comprehensive marketing and sales campaign

Tags for this Online Resume: Cell Culture, biotech, PCR, Electrophoresis, Spectrophotometer, Biologist

Knowledge and experience in analysis techniques and performing manual and automated operations such as bioanalysis of drug substances, biotechnology research, laboratory tritrations, electrophoresis and column chromatography techniques, cell cultivation in biotechnology, Aseptic techniques, Teacan automation, formulation automation, filtration and pipeting.