Sentences with phrase «cell electroporations»

Not exact matches

Using electroporation — in Corn's words, «we run an electric current and make holes in the cells» — he'll inject the complex and replacement parts through the cells» protective membrane.

«New innovative method for delivering genes into cells: Modification of cell physiological function with novel parallelized electroporation of mammalian cells by using electrostatic manipulation in a water - in - oil droplet.»

Associate Professor Rika Numano explained, «The W / O droplet electroporation technique has several advantages over conventional transfection techniques: small cell number required, as low as 1000 cells; small amount of DNA required, which makes it applicable for various cell types including neural cells; and changeable genome DNA.

They then used a method known as electroporation, in which cells are briefly exposed to an electrical field that makes their membranes more permeable, to quickly deliver these RNPs to the interior of the cells.

Expansion of embryonic and adult neural stem cells by in utero electroporation or viral stereotaxic injection.

For both BayGenomics parental ES cell line E14Tg2a.4 (also used for Sanger (SIGTR) lines) and Soriano parental ES cell line AK7, ultra low passage cells suitable for multiple electroporations and germline transmission are available.

Produced by electroporation of MT - 2 cells with pxxHIV - 1Lai proviral DNA.

To generate your own edited clonal hiPS cell lines, try one of our complete systems containing a Cas9 - sgRNA RNP complex delivery system (choose electroporation - based or gesicle - based delivery) and the Cellartis iPSC Single - Cell Cloning DEF - CS Culture Media cell lines, try one of our complete systems containing a Cas9 - sgRNA RNP complex delivery system (choose electroporation - based or gesicle - based delivery) and the Cellartis iPSC Single - Cell Cloning DEF - CS Culture Media Cell Cloning DEF - CS Culture Media Kit:

INO - 3112 is injected into a muscle followed by electroporation, a technique that uses controlled, millisecond electrical pulses that make it easier for the DNA plasmids to enter muscle cells.

This group cultured human CD34 + cells in StemSpan ™ medium and cytokines as a pre-stimulation step ahead of electroporation, before xenotransplantation into mice or additional in vitro culture.

Whether a protocol uses cell culture methods to expand edited cells for downstream assays, to increase the efficiency of lentiviral transduction or to let cells «rest» after electroporation, optimized culture conditions are key to maintaining cell viability and biological function.

Tissues targeted (as reported in animals): Primarily blood, immune, and stem cells removed from the body; localized electroporation to skin

Primarily blood, immune, and stem cells removed from the body; localized electroporation to skin

Cell culture steps were used by both groups to let cells recover after thawing and / or electroporation, and additionally to generate erythroid progenitor cells from edited CD34 + cells [1].

HV cells constitutively expressing the LacZ gene were generated by electroporation with a vector containing a CAG driven β - Geo cDNA followed by selection in G418 (150 µg / ml) for 2 wk.

When tested in retinal electroporation studies, DsRed reporter expression was induced in ~ 76 % of GFP + cells, and 100 % of DsRed + cells also expressed GFP.

Important reports from the Weiner lab include the first DNA vaccine studied for HIV as well as for cancer immune therapy of cutaneous T cell lymphoma, the early development of DNA encoded genetic adjuvants, including IL - 12, advances in gene optimization, and advances in electroporation technologies resulting in improved gene delivery.

Electroporation of cultured cells was carried out using the Neon Transfection System (Invitrogen).

Cell - Specific Targeting Strategies for Electroporation - Mediated Gene Delivery in Cells and Animals.

Following electroporation into R26 BirA cells, a cell line that expresses bacterial BirA ligase from the ROSA26 locus, hygromycin resistant clones (200 µg / ml) were expanded for Southern analysis to identify correct targeting events.

HV cells overexpressing Nanog were generated by electroporation with a vector containing the Nanog cDNA under the control of a CAG promoter and upstream of IRES Puro cassette followed by selection in puromycin (2 µg / ml) for 2 wk.

Typically, Addgene uses electrocompetent Stbl4 cells when performing amplifications, and we use electroporation to ensure a good transformation efficiency.

1 - 4 days following DNA injection and electroporation, embryonic brains are dissected, sliced, placed on a filter in culture medium and live imaged over night (Baffet et al., Methods Cell Biol., 2015).

Electroporation of investigator - provided construct into mouse embryonic stem cells / Service Request form plus documentation.

With intratumoral electroporation, tumor cell pores are opened when a mild electrical current is introduced directly through an electrode.

This causes what's called reversible electroporation, where these cells will transiently increase their ability to be permeable to foreign compounds, and so you're able to get the cancer drugs into the tumors and kill them.

Faculty research interests and expertise include mechanisms of metastasis in cancer; gene therapy (including electroporation) in cancer; cell and molecular biology of tooth eruption; pharmacology and toxicology of aquatic species; environmental and ecological toxicology; cellular ultrastructure; environmental risk assessment; analytical pharmacology and toxicology; diagnostic neurophysiology; pharmacology of pain and inflammation; exercise physiology; xenobiotic metabolism and disposition; neurochemistry; pulmonary pharmacology; cardiovascular disease; and cetacean morphology.