Sentences with phrase «cell expansion in»

Bone marrow - derived mesenchymal stem cells (BM - MSCs) are obtained by isolating mononucleated cells from a marrow aspirate by centrifugation on a density gradient, followed by adherent cell expansion in tissue culture.

Specially formulated to enhance single cell expansion in the cloning step of stem cell genome editing, StemFit Basic02 offers superior and stable growth performance, high colony forming efficiency and robust scalable cell expansion.

Not exact matches

Mr Blair also used his trip to California to woo researchers in stem cell science to come to the UK, after Mr Bush used his first presidential veto to block the further expansion of federal investment in this area of research.

In that paper, Weitzmann, Ofotokun, and their colleagues described bone loss similar to that observed in humans following the reconstitution of the T cell population in immune - compromised mice (similar to T - cell expansion following ARTIn that paper, Weitzmann, Ofotokun, and their colleagues described bone loss similar to that observed in humans following the reconstitution of the T cell population in immune - compromised mice (similar to T - cell expansion following ARTin humans following the reconstitution of the T cell population in immune - compromised mice (similar to T - cell expansion following ARTin immune - compromised mice (similar to T - cell expansion following ART).

In this study published in Cell Reports, Alexandra Van Keymeulen and colleagues generated a new transgenic line - allowing lineage tracing of ER + luminal cells to investigate luminal cell heterogeneity and identify the origin of ER + luminal cells and the mechanisms regulating their pubertal expansion and adult maintenancIn this study published in Cell Reports, Alexandra Van Keymeulen and colleagues generated a new transgenic line - allowing lineage tracing of ER + luminal cells to investigate luminal cell heterogeneity and identify the origin of ER + luminal cells and the mechanisms regulating their pubertal expansion and adult maintenancin Cell Reports, Alexandra Van Keymeulen and colleagues generated a new transgenic line - allowing lineage tracing of ER + luminal cells to investigate luminal cell heterogeneity and identify the origin of ER + luminal cells and the mechanisms regulating their pubertal expansion and adult maintenaCell Reports, Alexandra Van Keymeulen and colleagues generated a new transgenic line - allowing lineage tracing of ER + luminal cells to investigate luminal cell heterogeneity and identify the origin of ER + luminal cells and the mechanisms regulating their pubertal expansion and adult maintenacell heterogeneity and identify the origin of ER + luminal cells and the mechanisms regulating their pubertal expansion and adult maintenance.

As the smooth muscle cells grew, the team subjected them to a battery of mechanical stresses that mimic the expansion and collapse that such a vessel would ultimately experience in the heart.

Professor Taylor, who co-ordinated the research, says: «Our findings provide the very first insight into how biochar stimulates plant growth — we now know that cell expansion is stimulated in roots and leaves alike and this appears to be the consequence of a complex signalling network that is focussed around two plant growth hormones.

Previous work in the lab of CU Cancer Center investigator, Carol Sartorius, PhD, and others shows that progesterone aids the expansion of CK5 + cells.

When the team used the retinoid fenretinide along with anti-estrogen therapy in mouse models of breast cancer, they did not see the expansion of CK5 + cells previously seen with anti-estrogen therapy alone.

Meanwhile, recent human studies indicate that aging is associated with an increase in somatic mutations in the hematopoietic system, which gives rise to blood cells; these mutations provide a competitive growth advantage to the mutant hematopoietic cells, allowing for their clonal expansion — a process that has been shown to be associated with a greater incidence of atherosclerosis, though specifically how remains unclear.

Sheng Ding, PhD, a senior investigator in the Roddenberry Stem Cell Center at Gladstone and co-senior author on the study, adds, «This new cellular reprogramming and expansion paradigm is more sustainable and scalable than previous methods.

Protein channels in the cell's membrane release the excess water, preventing catastrophic expansion and bursting of the cell.

Blood testing showed an expansion in the numbers of CD19 - targeted CAR T - cells that coincided with the tumor's regression.

In particular, they found significant expansion of CD3 + CD8 + cytotoxic T - cells and CD3 + CD4 + helper T - cells in circulating blood, as well as increased CD4 + subsets of Th1, Th2, Th17 and Treg cells, indicating a pro-inflammatory responsIn particular, they found significant expansion of CD3 + CD8 + cytotoxic T - cells and CD3 + CD4 + helper T - cells in circulating blood, as well as increased CD4 + subsets of Th1, Th2, Th17 and Treg cells, indicating a pro-inflammatory responsin circulating blood, as well as increased CD4 + subsets of Th1, Th2, Th17 and Treg cells, indicating a pro-inflammatory response.

They found the CD4 + T - cells were globally expanded and activated during chronic ischemic heart failure, and that there was an expansion of memory T - cells in the spleen.

In a series of side - by - side comparisons, Mooney's team demonstrated that APC - mimetic scaffolds performed better than methods involving commercially available expansion beads (Dynabeads), which are currently used in clinical adoptive cell transfer approacheIn a series of side - by - side comparisons, Mooney's team demonstrated that APC - mimetic scaffolds performed better than methods involving commercially available expansion beads (Dynabeads), which are currently used in clinical adoptive cell transfer approachein clinical adoptive cell transfer approaches.

«In a single dose, APC - mimetic scaffolds led to two - to ten-fold greater expansion of primary mouse and human T cells than Dynabeads.

An APC - mimetic scaffold that was engineered to activate a specific type of CAR - T cell was able to generate higher numbers of the modified T cells over longer periods of culture than analogously designed expansion beads, and the resulting cells were similarly effective in killing the lymphoma cells in the mice.

Building on these findings, the researchers demonstrated the utility of their T cell expansion platform in a therapeutic model.

The study, «Anti-TNF drives regulatory T cell expansion by paradoxically promoting membrane TNF - TNF - RII binding in rheumatoid arthritis,» which will be published online June 6 in The Journal of Experimental Medicine, may help explain the divergent efficacies of different TNF - targeting drugs.

In this study, using a mouse transgenic approach, the scientists demonstrated that ILC2s express a checkpoint molecule, known as «PD - L1», that functions to control the expansion of allergy - inducing Th2 cells and the development of allergic pulmonary and gut tissue inflammation.

First author of the paper, Dr Christian Schwartz, a European Molecular Biology Organization Long Term Fellow in Professor Fallon's group, added: «It is fascinating that a small cell population such as the ILC2s can regulate the expansion of Th2 cells and thereby shape the whole outcome of an immune response — be it beneficial in case of parasitic infections, or detrimental as in the case of allergic responses.»

Simultaneous T cell antigen receptor (TCR) analysis in single cells and bulk subsets revealed that CD4 - TEMRA cells show marked clonal expansion compared with TCM and TEM cells and that most of CD4 - TEMRA were dengue virus (DENV)-- specific in donors with previous DENV infection.

«We have discovered the key role played by the TOR signalling pathway in driving the expansion of the endoplasmic reticulum, and sending a cell's factories into overdrive.

Vaccine immunology is poorly understood in pregnancy and Tfh cell expansion has been shown to be a predictor of response to influenza vaccination outside of pregnancy.

«There is a dramatic expansion of inhibitory T cells in the tumors after the infusion, much more significant than what you see without the CAR T cells,» O'Rourke said.

One of the primary causes of the decline in Grauer's gorilla numbers has been the expansion in artisanal mining for coltan (a key mineral used in the manufacture of cell phones and other electronics) and other minerals in the gorilla's range.

Nine patients were treated with tocilizumab, an immunosuppressant drug that blocks the effects of the inflammatory cytokine IL - 6, which have been found to spike during the most robust phase of the engineered cells» expansion in the body.

The approach enabled a wide range of studies of human brain development, including implicating a new class of neural stem cell recently discovered by the lab in the evolutionary expansion of the human brain and identifying how the mosquito - borne Zika virus may contribute to microcephaly in infants infected in utero.

Because the anticancer compound works by binding to troublesome expansions in DNA, the researchers decided to test whether it would work in cells and a mouse model of myotonic dystrophy type 1.

The researchers describe the successful application of their strategy in mice in «Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion,» which will be published online August 15 ahead of issue in The Journal of Experimental Medicine.

The beneficial responses were associated with an increase in systemic dendritic cells, which in turn increased the expression of interleukin 12 (IL - 12), which sustained expansion and anti-tumor effects of transferred T cells.

Even though STAT3 activity drives the expansion of MDSCs and is involved in immune responses mediated by the cells, they found that high levels of STAT3 activity actually prevent the differentiation of MDSCs to macrophages.

Tests over time showed expansion of the modified cells in the body that peaked between days seven and 10.

In studies of neural development in mice, Stahl found that TRNP1 produces a protein that determines whether neural stem cells self - replicate, leading to a balloonlike expansion of cortical surface area, or whether they differentiate into a plethora of intermediate stem cell types and neurons, thickening the cortex and forming more complex brain structureIn studies of neural development in mice, Stahl found that TRNP1 produces a protein that determines whether neural stem cells self - replicate, leading to a balloonlike expansion of cortical surface area, or whether they differentiate into a plethora of intermediate stem cell types and neurons, thickening the cortex and forming more complex brain structurein mice, Stahl found that TRNP1 produces a protein that determines whether neural stem cells self - replicate, leading to a balloonlike expansion of cortical surface area, or whether they differentiate into a plethora of intermediate stem cell types and neurons, thickening the cortex and forming more complex brain structures.

Unlike in previous CGD trials, the cells not only kept growing but also went through an «unexpected expansion» of numbers, Grez reported.

Normally, MIR506 plays an important role in regulating cell behavior; adequate levels help the cells function normally, while decreased levels trigger cell growth and expansion occurring in tumors.

In cancer, a state marked by increased protein translation and biomass expansion, HSF1 is activated and binds to numerous genes throughout the genome, as seen in the heat map of HSF1 ChIP - Seq read density in M0 - 91acute myeloid leukemia cells (DMSO, far leftIn cancer, a state marked by increased protein translation and biomass expansion, HSF1 is activated and binds to numerous genes throughout the genome, as seen in the heat map of HSF1 ChIP - Seq read density in M0 - 91acute myeloid leukemia cells (DMSO, far leftin the heat map of HSF1 ChIP - Seq read density in M0 - 91acute myeloid leukemia cells (DMSO, far leftin M0 - 91acute myeloid leukemia cells (DMSO, far left).

By combining SNP - targeting allele - specific silencing and gain - of - function approaches, we showed that a 46 - glutamine expansion in human HTT was sufficient for a dominant - negative effect on spindle orientation and changes in the distribution within the spindle pole and the cell cortex of dynein, p150Glued and NuMA in neural cells.

Using a novel technique to genetically modify T cells for adoptive transfer, Carl June, Michael Kalos, David Porter, Bruce Levine, and colleagues at the University of Pennsylvania School of Medicine achieve clinical responses in patients with chronic lymphocytic leukemia, including two complete, durable (one year) clinical responses, accompanied by in vivo expansion and long - term functional persistence of gene - modified cells.

Specific duties will include basic and advanced polymer chemistry protocols, cell culture and expansion, hydrogel biomaterial characterization, physical characterization of biomaterials, assembly of 3D constructs using biofabrication and bioprinting techniques, collection and organization of data, and assisting in the preparation of reports and manuscripts.

A few years ago, Dr. Funderburgh and other University of Pittsburgh researchers identified stem cells in a layer of the cornea called the stroma, and they recently showed that even after many rounds of expansion in the lab, these cells continued to produce the biochemical components, or matrix, of the cornea.

For the expansion and differentiation of human keratinocyte stem cells for permanent skin restoration in victims of extensive burns.

A further 25 days in medium containing FGF2 and Noggin mediated the derivation and expansion of RPCs which had a similar global gene expression pattern to cells of the embryonic day 14 (E14) mouse retina.

The discovery of human oRGs» self - renewing niche and remarkable generative capacity reinforces the idea that these cells may have been responsible for the expansion of the cerebral cortex in our primate ancestors, the researchers said.

Expansion of embryonic and adult neural stem cells by in utero electroporation or viral stereotaxic injection.

These so - called «living drugs» — injected T cells genetically modified to better recognize and kill tumor cells through a perpetual process of cell renewal and expansion — are revolutionizing cancer treatment, with the first two FDA approvals of such gene - altering therapies occurring in just the last two months.

Benedetta Artegiani (Calegari, TUD)-- «A Transitory Overexpression of Cdk4 / CyclinD1 Triggers a Greater Expansion of Neural Stem Cells and Neurons in the Adult Mouse Brain» (2012)

In his spare - time, Riggs promotes the expansion of the Student Society for Stem Cell Research (SSSCR).

To culture embryonic stem cells on LN521 enables easy and efficient expansion of your hESCs and iPSCs and provides a biorelevant in vitro stem cell niche.