Sentences with phrase «cell genome sequencing»

Conclusions: The method is quick and easy to apply, straightforward to interpret, and represents a substantial cost saving compared to single - cell genome sequencing techniques.

«Multiclonal invasion identified in early breast tumors by topographic single cell genome sequencing»

Navin's team developed Nuc - Sec as a single - cell genome sequencing method and applied it to study how cell mutations occur in both types of breast cancer.

Not exact matches

To determine how the cells switch from one type to another, they took three human uterine carcinosarcoma samples and sequenced the genomes of cells in two parts of each tumor: the carcinoma and sarcoma components.

Lacks» family was never asked for permission to harvest her cells, an ethical violation made worse in March when German researchers published the sequence of the HeLa genome without asking.

The man responsible for one of the original sequences of the human genome as well as the team that brought you the first living cell running on human - made DNA now hopes to harness algae to make everything humanity needs.

«These predictive algorithms seem to do a good job when CRISPR is performed in cells or tissues in a dish, but whole genome sequencing has not been employed to look for all off - target effects in living animals,» says co-author Alexander Bassuk, MD, PhD, professor of pediatrics at the University of Iowa.

When researchers sequenced the sponge's genome in 2010, they found genes that help individual cells cooperate as a group: how to divide, send signals to one another, and distinguish between friends and outsiders.

Slight modifications in their genome sequences play a crucial role in the conversion of pluripotent stem cells into various differentiated cell types.

An analysis of the HPV16 genome from 5,570 human cell and tissue samples revealed that the virus actually consists of thousands of unique genomes, such that infected women living in the same region often have different HPV16 sequences and variable risks to cancer.

To address this gap in knowledge, Mirabello and Schiffman teamed up with co-senior author Robert Burk of Albert Einstein College of Medicine to sequence the whole genomes of 5,570 HPV16 - infected cell and tissue samples from women around the world and to identify associations between HPV16 genetic variants and the risk of cervical precancer and cancer.

Scientists investigating the earliest stages of cancer development used an exquisitely sensitive sequencing method capable of detecting DNA mutations present in as few as 1.6 per cent of blood cells, to analyse 15 locations in the genome, which are known to be altered in leukemia.

There, some of the added DNA was swapped with the matching target sequences in the cells» genomes.

Sequencing the genome of one such organism, King and her colleagues found genes that code for pieces of the same proteins used for the binding of cells and communication between cells in animals — functions that would be unexpected in such an organism.

«Whole - genome, single - cell [sequencing] costs about the same as bulk - cell sequencing,» says BGI's Peng.

Researchers can send their cells to SingulOmics for preparation and genome amplification, and then have the amplified DNA sent to Novogene for sequencing.

The diversity of genome amplification options underscores the importance of having a clear experimental question in mind before starting a single - cell sequencing project.

For DNA sequencing, that requires first amplifying each cell's genome in order to construct a library for sequencing.

«Whole - genome, single - cell [sequencing] costs about the same as bulk - cell sequencing.»

The result was an extremely high - throughput sequencing protocol that, in a single run, can cover portions of the genomes of over 50,000 microbial cells (2).

Though these barcodes are synthesized differently from 10x Genomics» RNA - sequencing barcodes, their purpose is similar: to link each cell's amplified genome pieces to a unique identifying sequence.

The man who first sequenced the human genome and designed the first synthetic cell explains why simple algae — and some genetic engineering — may hold the key to our future

Cell - free genomic DNA isolated from human cells was cleaved with preassembled, recombinant Cpf1 RNPs and subjected to whole - genome sequencing.

Roughly speaking, ChIP sequencing can be compared to a microscope, which enables us to observe the presence of different cell components in the entire genome at a given time.

The six previously known fly rhodopsins account for the full function of photoreceptor cells in the fly's eyes, so although the fruit fly genome contained the sequence of a seventh rhodopsin, the role of Rh7 was unclear.

Current genome sequencing techniques involve the chemical disintegration of samples of red blood cells from infected patients to obtain parasite DNA, which are then sequenced.

«The DNA sequence itself tells us very little about how cells actual decodes the DNA, and to understand this we need to map out which cell components are present in different parts of the genome at a specific time.

DNA sequencing is used for mapping the sequence of the base pairs, which our DNA consists of, and ChIP sequencing is a derived method in which the sequences are used to determine the presence of different cell components in the genome at a given time.

Not only has the parasitic micro jellyfish evolved a stripped - down body plan of just a few cells, but via data generated at the KU Medical Center's Genome Sequencing Facility researchers also found the myxozoan genome was drastically simplGenome Sequencing Facility researchers also found the myxozoan genome was drastically simplgenome was drastically simplified.

Focusing on DNA from chloroplasts and mitochondria — energy - producing structures in cells that have their own genomes — the researchers produced 49,000 genetic sequences.

The team integrated three, complementary gene sequencing approaches to look for mutations in tumor cells from SS patients: whole - genome sequencing in six subjects, sequencing of all protein - coding regions (exomes) in 66 subjects, and comparing variation in the number of copies of all genes across the genome in 80 subjects.

Professor Gianni Liti, a senior author on the paper from the Institute for Research on Cancer and Ageing, Nice, said: «We were able to study the evolution in time by combining genome sequences of the cell populations and tracking the growth characteristics of the yeast cells.

The genomes of these 377 bacterial isolates, plus an additional 107 single bacterial cells from roots of A. thaliana, were then sequenced, assembled, and annotated at the JGI.

«This study demonstrates the potential of combining functional profiling of cells with the characterizations of cancer genomes via next generation sequencing,» said co-senior author Jill P. Mesirov, PhD, professor and associate vice chancellor for computational health sciences at UC San Diego School of Medicine.

In order to locate all gene switches, the Freiburg research team used modern sequencing methods to examine the entire genome — DNA, epigenetic markers and RNA — during the development, maturation and disease of human cardiac muscle cells.

A human genome contains roughly 3 billion nucleotides in linear sequence, and that is the same, again to a large approximation, in every cell type throughout life.

Moreover, as genomes of other animals are sequenced, «there will be a lot of biology that will be clarified,» says Tom Pollard, a cell biologist at Yale University in New Haven, Connecticut.

The study was performed using single stem cells cultivated to provide sufficient DNA for whole genome sequencing.

Without the genome sequence of Lacks» healthy cells or that of her original tumor, it is difficult to trace the origin of these alterations.

As part of ENCODE, 32 institutions did computer analyses, biochemical tests, and sequencing studies on 147 cell types — six fairly extensively — to find out what each of the genome's 3 billion bases does.

To study excision repair in cells, Sancar, Selby and colleagues recently developed a new technique, XR - seq, which allows investigators to isolate and sequence the small lengths of adduct - damaged DNA that are snipped from the genome during the excision repair process.

When researchers sequenced the chimpanzee genome in 2005, the biggest difference between it and the human genome was the extinct PtERV1 retrovirus, which inserted its DNA into the cells it infected like HIV does today.

Derived mostly from human embryonic kidney 293T (HEK293T) and HeLa cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gcell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gCell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gene.

After the sorting, we performed whole genome amplification on individual aggregates then sequenced the 16S rRNA marker genes to identify cells comprising the aggregate.

In previous studies, including recent genome sequencing of cancer patients, human SETD2 has been implicated in several cancer types, especially in renal cell carcinoma — the most common kind of kidney cancer.

«Scientists sequence genome of worm that can regrow body parts, seeking stem cell insights: Worm's genome could lead to better understanding of its regenerative prowess, advance stem cell biology.»

The first study to sequence and analyze the entire genome of a HeLa cell line, along with access to its sequence data, has been published Aug. 7 in its final version, by G3: Genes Genomes Genetics, an open - access, scientific journal of the Genetics Society of America.

The new controlled access policy for full genome sequence data from HeLa cells will give the Lacks family the ability to have a role in work being done with the HeLa genome sequences and track any resulting discoveries.

Under the policy, biomedical researchers who agree to abide by terms set forth in the HeLa Genome Data Use Agreement will be able to apply to NIH for access to the full genome sequence data from HeLa Genome Data Use Agreement will be able to apply to NIH for access to the full genome sequence data from HeLa genome sequence data from HeLa cells.

Dr. Steinmetz and his team found the genome of the HeLa cell line that they sequenced differs dramatically from a normal human genome sequence.