Sentences with phrase «cell genomic sequencing»

The researchers used single cell genomic sequencing, a very useful and still comparatively new capability.

In a recent proof - of - concept experiment, Abate and his colleagues performed single - cell genomic sequencing on a sample of seawater.

Not exact matches

Using advances in genomic sequencing, the human microbiome, proteomics, informatics, computing, and cell therapy technologies, HLI is building the world's most comprehensive database of human genotypes and phenotypes as a basis for a variety of commercialization opportunities to help solve aging related disease and human biological decline.

Zhang's group is moving beyond bulk sequencing of tumor samples and is now conducting genomics on single cells.

Called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing, this new technique complements existing genomic tools, such as ChIP - seq (chromatin immunoprecipitation followed by sequencing), and offers some additional benefits.

«However, because SIF - seq only requires DNA sequence from a mammal and can be used in a variety of cell types, it should be possible to compare the neuronal enhancers present in a large genomic region from human to the neuronal enhancers present in the orthologous chimpanzee region.

As costs come down and methods become more standardized, experts in the field expect single - cell sequencing to become the new model for both transcriptomic and genomic studies.

Cell - free genomic DNA isolated from human cells was cleaved with preassembled, recombinant Cpf1 RNPs and subjected to whole - genome sequencing.

A multicenter team of researchers reports that a full genomic analysis of tumor samples from a small number of people who died of pancreatic cancer suggests that chemical changes to DNA that do not affect the DNA sequence itself yet control how it operates confer survival advantages on subsets of pancreatic cancer cells.

If it was a mixture of microbes, it would be the perfect sample for a technique she and her colleagues were developing called Hi - C sequencing, which disentangles the genomic sequences of a community of microbe species using formaldehyde to link DNA fragments within the same cell.

Derived mostly from human embryonic kidney 293T (HEK293T) and HeLa cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gcell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gCell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gene.

The advent of genomics and rapid sequencing techniques has seen HeLa cells used in numerous large - scale studies of gene function and expression.

Without this approach, the results of genomic sequencing would have been swamped with DNA from normal cells, making it difficult to detect cancer - linked DNA errors, Papp says.

Enzymatic amplification of beta - globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia

These CRISPR sequences act as a form of genomic memory that can be accessed to defend the cell when it is invaded by plasmids or phages that contain the recorded sequences.

Engineered cells are then validated via next - generation sequencing and analysis of the modified genomic loci with custom analytical tools developed by our bioinformatics team.

His thesis was entitled «Elucidating cancer evolution using single - cell sequencing and comparative genomics.»

Using advances in genomic sequencing, the human microbiome, proteomics, informatics, computing, and cell therapy technologies, HLI is building the world's most comprehensive database of human genotypes and phenotypes as a basis for a variety of commercialization opportunities to help solve aging related disease and human biological decline.

These types of investigations have been enabled by key technologies, including single - cell assays, high - throughput genomic sequencing, and improved bioinformatic algorithms.

In a study being published online today in the journal Cell, researchers in the laboratory of Gladstone Senior Investigator Benoit Bruneau, PhD, employed stem cell technology, next - generation DNA sequencing and computing tools to piece together the instruction manual, or «genomic blueprint» for how a heart becomes a heCell, researchers in the laboratory of Gladstone Senior Investigator Benoit Bruneau, PhD, employed stem cell technology, next - generation DNA sequencing and computing tools to piece together the instruction manual, or «genomic blueprint» for how a heart becomes a hecell technology, next - generation DNA sequencing and computing tools to piece together the instruction manual, or «genomic blueprint» for how a heart becomes a heart.

An international team of researchers has reported the complete genomic sequence of Anabaena, a cyanobacterium — or blue - green algae with beadlike cells.

Scientific programs include: human genomic sequencing and analysis, synthetic genomics and exploration of new vaccines using this technology, and environmental and single cell genomics to explore the vast unseen world of microbes living in the human body, the ocean, soil and air.

Sequencing of DNase I hypersensitive sites in (125 cell types), FAIRE analysis of reduced nucleosome crosslinking (25 cell types), and genomic DNase I footprinting (41 cell types).

Cell biology has continued to expand ever since, extending its impact on clinical medicine and pharmacology while drawing on new technologies in bioengineering, high - resolution imaging, massive data handling, and genomic sequencing.

The Swedish Biodiversity Program aims to target biodiversity of bacteria, viruses, archaea and eukaryotes and combinations thereof to promote large research projects aiming to uncover and understand genomic variability in nature by use of massively parallel sequencing and microbial single cell analysis.

Sequencing has also demonstrated that each patient's cancer cells may carry different combinations of mutations, a phenomenon called «genomic heterogeneity» that may help explain why some patients respond poorly to treatment.

The scientists developed a method to visualize the repair process based on genomic sequencing, and with it found good evidence for their hypothesis: In the cells that lacked GreA, less DNA degradation surrounded the sites of DNA breaks.

Genetic Analysis of HIV Genomic DNA Sequences from Phenotypic and Antigen - specific CD4 + T Cell Subsets

Analysis of viral genomic and human mitochondrial sequences revealed that all previously characterized XMRV strains are identical and that the archival RNA had been contaminated by an XMRV - infected laboratory cell line.

Genomics in the search for novel signature DNA sequences: We are using our combined expertise on evolutionary phylogeny, prokaryote cell physiology and comparative genomics to identify new signature sequences.

Since the first single - cell RNA - sequencing (scRNA - seq) study was published in 2009, many more have been conducted, mostly by specialist laboratories with unique skills in wet - lab single - cell genomics, bioinformatics, and computation.

PGBD5 was physically associated with genomic PSS sequences that were also sufficient to mediate PGBD5 - induced DNA rearrangements in rhabdoid tumor cells.

Together with Anna - Maria Divne, I am setting up and establish methods for single cell genomics, from single cell sorting, to whole genome amplification, target screening and sequencing library preparation.Why can you find me on this homepage?

Specifically, we have generated clusters of transcripts that behave the same way under the entire spectrum of the sixty - seven experimental conditions; we have assembled genes in groups according to their time of expression during successive days of ES cell differentiation; we have included expression profiles of specific gene classes such as transcription regulatory factors and Expressed Sequence Tags; transcripts have been arranged in «Expression Waves» and juxtaposed to genes with opposite or complementary expression patterns; we have designed search engines to display the expression profile of any transcript during ES cell differentiation; gene expression data have been organized in animated graphs of KEGG signaling and metabolic pathways; and finally, we have incorporated advanced functional annotations for individual genes or gene clusters of interest and links to microarray and genomic resources.