Sentences with phrase «cell growth medium»

A steady flow of cell growth medium passes through the cage to maintain near - constant conditions.

Not exact matches

Some reports, such as the Open Philanthropy Project's Animal Product Alternatives report and Van der Weele & Tramper (2014) suggest it is unlikely that cultured meats will become cost - competitive with conventional meat.98, 99 One important contributing factor in this conclusion, which is cited in these reports, is the minimum costs of the growth medium necessary for culturing the desired cells.

Varghese and her team showed that they could control the differentiation of human pluripotent stem cells into functional osteoblasts — bone - building cells — simply by adding the molecule adenosine to their growth medium.

The good doctor, who has made a career in the field of artificial - tissue generation, says that in the future we'll be sprinkling a few «starter cells» into our meat machine before we go to bed and adding a cup or two of «growth medium.»

A few starter cells scraped off one's arm, a night stewing in growth medium, and voilà: a vehicle for passive aggression more poetic than the world has seen since the days when it was still possible to irritate people to the point that they'd burn you at the stake in front of a crowd.

To shock the plants, Cuello applied a 30 to 100 milliamp current to the growth - medium of plants grown hydroponically, or, in the case of barrel medic, to the solution surrounding the cell cultures.

A team of developmental biologists led by Hans Schöler and Karen Hübner at the University of Pennsylvania placed densely packed clusters of stem cells from mouse embryos in a petri dish, using fetal calf serum as a growth medium and adding a gene protein that turns green when germ cells form.

One of the main challenges was to ensure that all cells were equally supplied with the growth medium, so they are evenly distributed and do not form clumps.

Their stem cells, which can mature into any type of tissue, were isolated and cultured in a dish around dots of gel - like growth medium.

Scientists in Germany last year reported that they could transform adult mouse spermatogonial progenitors into cells genetically resembling ESCs by using a different growth medium for just a few weeks.

By adding the plant hormone auxin to the medium that supports growth of tomato cells, they can speed up the plant's growth, ultimately accelerating the pace of their research.

A more alkaline growth medium, for example, made the autoinducer less stable, slowing activation of the cell - suicide gene and increasing the population.

«All we know at the moment is that it relies on calcium, because when we took calcium out of the growth medium, cell death did not occur,» he says.

Metastatic variant of MCF7 (MCF7 - BoM2d cells) was suspended in DMEM - F12 medium supplemented with 2 % of B27 supplement, 0.4 % BSA, 4 μg / mL insulin, 20 ng / mL basic fibroblast growth factor (bFGF), and 20 ng / mL EGF (Invitrogen).

These include: acid hydrolysis and monosaccharide composition using gas - liquid chromatography [3], microscopic observation of xylem stem sections [4, 5], seedling growth on medium containing cell wall hydrolyzing enzymes [6] and Fourier - Transform Infrared (FT - IR) microspectroscopy [7, 8].

Cells were spun at 300g for 5 min, resuspended in 8 ml EC growth medium and plated in two wells of a six - well plate coated for 2 h at 37 °C with type I Collagen (isolated from rat tail tendons).

To make HT22 growth conditioned medium, cells were grown in DMEM with 10 % fetal calf serum.

PC12 cells were plated in growth conditioned medium (CM) prepared from HT22 cells incubated overnight plus or minus 100 nM J147, and as controls fresh DMEM plus 100 nM J147 or fresh DMEM plus 50 ng / ml NGF.

Knowing that autophagy in animal cells was stimulated by starvation, he grew some of his mutant yeast in growth medium that was nutritionally deficient, and he then examined the cells under a simple light microscope.

The following day, the growth conditioned medium was collected and centrifuged at 10,000 × g to remove detached cells.

By adding the plant hormone auxin to the medium that supports the growth of the cells, they can speed up the plant's growth, ultimately accelerating the pace of their research.

For monolayer differentiation, confluent iPS cells were cultured in the HFF culture medium without any growth factors.

Cells were cultured on irradiated mouse embryonic fibroblasts (MEF) in DMEM / F12 culture medium supplemented with KnockOut serum replacer (20 %), non-essential amino acids (0.1 mM), L - glutamine (1 mM), ß - mercaptoethanol (0.1 mM) and 100 ng / ml zebrafish basic fibroblast growth factor.

Now Whitehead Institute researchers have designed a growth medium that more closely resembles the cells» environment in the body — and demonstrated that, relative to decades - old recipes that have remained the workhorses of cell culture studies, it significantly alters the cells» inner workings.

More specifically, addition of the PI3K inhibitor LY294002 to the growth medium of mouse muscle cells produces enhanced nuclear localization of FOXO1 and increased MAFbx mRNA transcription (52, 53).

The problems would surely stem from console manufacturers who can make profitable growth once their machine is established (see Nintendo) and a universal medium for any competitive industry isn't exactly hard, it's almost impossible as clearly seen in the cell phone market with various software formats and even battery adapters varying from model to model.