For the first time, super-resolution live -
cell imaging allows researchers to capture short videos of fast - moving cellular processes while discerning the precise location of nearly each individual protein they are studying.
Not exact matches
The latest in live -
cell microscopy — multiphoton
imaging, light - sheet techniques, and technology borrowed from Raman spectroscopy —
allow researchers to study living
cells in more detail with less effort.
Huerta also calls attention to fluorescent resonance energy transfer, which he says, «really
allowed us to start
imaging processes in
cells as they occur.»
The results will
allow, for the first time, the
imaging of nanoscale processes, such as the engulfment of nanoparticles into
cells.
The technique could also be modified for microscopy,
allowing imaging of photosynthesis inside the plant
cells.
«The
imaging system, developed by Dr. Young while at the University of Pittsburgh, pinpoints the spatial and temporal location of HIV -1-infected
cells in the body,
allowing us to observe HIV - 1 replication in real - time and to essentially see HIV - 1 reservoirs in latently infected
cells and tissues,» Dr. Khalili explained.
The team used an
imaging technique that
allowed them to view the responses of over 100 Kenyon
cells at a time and, importantly, quantify their results.
Using a powerful
imaging technique that
allowed the scientists to track the presence and movement of parasites in living tissues, the researchers found that Toxoplasma infects the brain's endothelial
cells, which line blood vessels, reproduces inside of them, and then moves on to invade the central nervous system.
First,
imaging experiments revealed that Tregs have a close relationship with the stem
cells that reside within hair follicles and
allow them to regenerate: the number of active Tregs clustering around follicle stem
cells typically swells by three-fold as follicles enter the growth phase of their regular cycle of rest and regeneration.
«This type of
imaging is so important because it
allows us to see and measure relationships between
cells and their environment,» Cohen said.
Building on traditional SIM technology, the iSIM
allows real - time, 3 - D super resolution
imaging of small, rapidly moving structures — such as individual blood
cells moving through a live zebrafish embryo.
A new method of
imaging cells is
allowing scientists to see tiny structures inside the «control centre» of the
cell for the first time.
«Scientists design bacteria to reflect «sonar» signals for ultrasound
imaging: New technology may one day
allow doctors to image therapeutic bacterial
cells in patients.»
Time lapse images collected on a Zeiss Axiovert 200M fluorescence microscope in the core
imaging facility of IGB
allowed real - time monitoring of the redox state in the cytosol and mitochondria of live
cells.
In their January publication in the Journal of Neurophysiology, researchers Michael A. Gaffield, Ph.D., Samanta B. Amat, and Jason M. Christie, Ph.D., describe the modification of
imaging and behavior techniques
allowing them to monitor the activity of Purkinje
cells over seven consecutive weeks during the course of a motor association task, in particular, the licking behavior of mice.
The new
imaging technique will
allow researchers to see the effects of novel drugs on this final stage in the parasite's invasion strategy, researchers report online on this week in
Cell Host & Microbe.
The researchers used two - photon calcium
imaging and patch - clamp electrophysiology, two sophisticated technologies that
allowed the researchers to record the signals from individual brain
cells.
They found that injecting into the carotid artery breast cancer
cells that express markers
allowing them to enter the brain —
cells labelled with bioluminescent and fluorescent markers to enable tracking by
imaging technologies — resulted in the formation of many metastatic tumors throughout the brain, mimicking what is seen in advanced breast cancer patients.
The injected
cells express markers that
allow them to enter the brain and are labelled with bioluminescent and fluorescent markers to enable tracking by
imaging technologies.
Inverted microscopes
allow the
imaging of live
cells in culture acquiring either still photos or time - lapse movies.
A clearing protocol adapted for embryos
allows deeper
imaging, including zeroing in on the heart as shown here.MINGFU WUThe average human is made up of more than 30 trillion
cells, not counting the hefty microbiome he or she carries.
This
allows for 4D living
cell imaging, where experiments limited to seconds or minutes on other
imaging platforms can be extended to hours or even days.
CODIM systems use standard workflow samples, acquiring images with the lowest light level,
allowing living
cell nanoscale
imaging.
Today, analyzing and editing genomes, proteomes and metabolomes has become a standard for many model systems;
imaging beyond the diffraction limit of light and new technologies for studying protein structures provide insights deeper than ever before; the characterization of large populations of
cells or organisms brings unprecedented statistical power; and studying nearly all organisms of an ecosystems as a whole
allows generating comprehensive models.
Detection of the signal via an electron multiplication CCD camera
allows image capture at speeds to hundreds of frames per second as demanded today by live
cell imaging.
Lucas Pelkmans and colleagues use much brighter probes,
allowing them to perform rapid and robust low - magnification
imaging of many more
cells, quantify low - level expression accurately and also query very short RNA transcripts.
Researchers at Columbia University have made a significant step toward breaking the so - called «color barrier» of light microscopy for biological systems,
allowing for much more comprehensive, system - wide labeling and
imaging of a greater number of biomolecules in living
cells and tissues than is currently attainable.
These range from visual stimulation experiments that
allow us to tap into the specific sets of retinal ganglion
cells that are most vulnerable early in the disease, to the evolution of new
imaging techniques, largely thanks to Alf Dubra and Vivek Srinivasan's work in those areas, and the ability to image retinal ganglion
cells and their component parts like their axons which degenerate very early in glaucoma.
The unique location, large size, and specific arrangement of the Platynereis mesodermal midline
cells allowed their unambiguous identification after whole - mount in situ hybridization (WMISH) and thus expression profiling by confocal
imaging.
Functional
imaging using in vivo confocal microscopy which
allows the analysis of vaso - activity phenomena during hypoxia, of ischaemia reperfusion events, or of homing
cells in pathologic processes, such as tumours or inflammatory diseases.