Sentences with phrase «cell injection into»

(However, it recovered on its own and not because of stem cell injection into his hip joints.)

Could stem cell injection into the affected leg offer therapeutic benefit?

Not exact matches

Immune cells modified by CRISPR - Cas9 were inserted into a lung cancer patient at the West China Hospital in Chengdu in the hopes that they'll be able to fight tumors, and 10 people total will receive injections of CRISPR re-engineered cells in order to assess the method's safety.

By using engineered zinc - finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one - cell rat embryo leads to a high frequency of animals carrying 25 to 100 % disruption at the target locus.

Following injection into the retina of mice, the researchers could see fluorescent green concentrating in RPE cells.

The researchers have now provided further evidence for this new theory by showing that the abnormal protein coded for this genetic disorder can be transmitted to normal animals by the injection of diseased cells into their brain.

The research team found that injection of CAR T cells into 79 immunodeficient mice arrested the growth of GBM in 60 % of them.

Using cells from cadavers, doctors have been experimentally transplanting pancreatic islets into humans for decades, but as many as 60 percent of the transplanted islets die immediately because they are cut off from their blood supply and are killed by an immune response due to direct injection into the bloodstream, and those that survive the transplant usually die within several months.

Clinical trials have shown that injection of human umbilical stem cells, or hUTC, into the retina helps preserve and restore vision in macular degeneration patients.

The researchers then injected 60,000 of the cells into the spinal columns of rats that had been paralyzed by the injection of a virus.

Infertility treatments once considered revolutionary are now commonplace: If a man has a low sperm count, sperm cells can be retrieved from a testicle for direct injection into an egg's cytoplasm.

After tracking the injected cells in the mice for more than 10 days using bioluminescence and fluorodeoxyglucose (FDG)- positron emission tomography (PET) imaging, they found that the injection of the senescent cells into the knee region caused leg pain, impaired mobility and characteristics of osteoarthritis, including damage to surrounding cartilage, X-ray changes, increased pain and impaired function.

Currently direct injection of genes into the cancer mass is being attempted, but it is unlikely that it will work because all the cells must be hit, which is very unlikely.

When injected into mice that were then given a subsequent injection of lymphoma cells, the 3D vaccine generated a potent immune response and delayed tumor growth.

An injection of SLex into the bloodstream should block the E-selectin on the surface of the blood vessels and so hide the chemical message that alerts the white blood cells.

While assisted reproductive techniques such as intracytoplasmic sperm injection (ICSI)-- which involves the direct injection of sperm into the egg cell — can overcome head or tail abnormalities in sperm, recent animal research suggests that fertility doctors must use these techniques with care.

Moreover, injection of 5E5 CAR T cells into mice with leukemia or pancreatic cancer reduced tumor growth and increased survival.

Albertini cites a popular IVF technique known as intracytoplasmic sperm injection, or ICSI, in which sperm is injected by needle right into the middle of an egg cell.

To test the delivery method, researchers divided 34 rats — all who received injections of cancerous cells into their brains — into different groups.

In contrast, injections of the cells into immunologically unmatched recipients (called an allogeneic transplantation) caused the body to mount a stronger immune response.

The research team then tested RMC - 1 in cultured human airway epithelial cells and mouse models of asthma (via injection into the animals» peritoneal cavities).

Importantly, the PLK4 inhibitor was able to cross the blood - brain barrier, which means that the drug can target cancer cells in the brain even when taken orally, avoiding the need for injection into the spinal fluid.

Injections of the cells into mice triggered no tumors, she also reported, and the cells survived for more than 6 months when transplanted into monkeys.

Ten people will receive injections into the injury site of hESC - derived oligodendrocyte progenitor cells, which stimulate the growth of new and severed nerves and recoat damaged nerves with myelin.

Injecting the TRAIL - expressing stem cells into the carotid artery, a likely strategy for clinical application, led to significantly slower tumor growth and increased survival, compared with animals receiving unaltered stem cells or control injections.

Subsequent experiments carried out at the Laboratory of Cell Biology in Rome by Luigi Aloe and one of us (Levi - Montalcini) demonstrated that this remarkable transformation can also take place in the intact animal: repeated injections of NGF into rat fetuses, continued for two to three weeks after birth, resulted in the differentiation of chromaffin cells into sympathetic neurons in the core of the adrenal gland!

CSCs were prepared from 231BrM and CN34BrM cells that were infected with lentivirus carrying with or without miR -7-2, and they were transplanted into nude mice through intracardiac injection followed by monitoring metastatic tumor growth in the brain.

We then examined the metastatic ability of these cells by implanting various doses of CSCs into mice via intracardiac injection.

We offer quality custom generation of a wide range of mouse models developed by microinjection of Sv129 / Pas or C57BL / 6N ES cells into blastocysts or by DNA pronuclear injection.

Other research at U-M is developing new options for treating brain cancer through immunotherapy — harnessing the immune system to attack cancer cells once an injection of a particular gene therapy is delivered into the brain tumor.

Four hours later, mice will receive bone marrow cells in PBS by intravenous injection into the tail vein.

Injections of iPSC - EPCs did not however have significant effect on tumor growth or on overall survival, but transducing cells with a baculovirus expressing CD40L, a member of the TNF gene family which can induce apoptosis [6, 7], and injection into the breast cancer lung metastasis, increased levels of pro-apoptotic cytokines in lung tissues, indicating the induction of apoptosis by CD40L carried by the EPCs (See figure).

Repeated injections of cells into the immune - normal mice led to more rapid cell death, indicating that the immune system was becoming more efficient at recognizing and rejecting the cells.

High throughput injection of poly (A) + RNAs into Xenopus oocytes (in collaboration with Biopredic International) to obtain cells expressing molecules characteristic of certain cell types on their surface.

Dr. Keristead created oligodendrocytes from hESCs, and showed that an injection of these cells into rats with spinal cord injuries could significantly improve their motor functions.

Second, the researchers observed that the stem cell injection coaxed the body into producing more of its own cells in the eye, which multiplied the therapeutic effect.

For intracardiac injections, cells were harvested from subconfluent culture plates, washed with phosphate - buffered saline (PBS), and resuspended at 106 / ml (1833) or 5 × 106 / ml (SCP28) in PBS; 0.1 ml of the suspended cells was injected into the left cardiac ventricle using 30 - gauge needles.

In theory, these sensor — cells could then be injected back into a patient for monitoring, replacing a series of invasive blood samples with a one - time injection.

The table shows the numbers of resultant embryos scored as low -, medium -, and high - contribution chimeras following the injection of fractionated HV cells into Rosa26 LacZ blastocysts.

The pluripotent properties of ES cells can be demonstrated by in vitro differentiation or by reintroduction of these cells back into chimeric embryos by blastocyst injection or morula aggregation.

The experimental metastasis assay we detail here includes tail - vein injection of cancer cells into the mouse and determination of the resulting secondary organ colonization, primarily in the lung, 10 d post dosing.

The cells will be injected into the patients» spinal fluid using a standard injection procedure, performed routinely in hospitals worldwide.

Research has shown that the injection of stem cells into the hands of Scleroderma patients reduced swelling and caused a return of normal color.

Injection of mouse embryonic stem cells into blastocysts / Service Request form plus documentation.

To demonstrate Amrose functionality in an established higher model system, Amrose v0 — v4 variants were cloned into the pCS2 + vector system to synthesize mRNA by in vitro transcription, which was used for injection into one - cell stage zebrafish embryos.

There are numerous, diverse mechanisms that allow a low frequency of gene movement between species, such as accidental fusion of cells between different species, or the injection of DNA by bacteria into plant cells.

Cytokine release syndrome occurs when many cytokines get released into the serum upon CAR T - cell injection; patients can get high fevers and sometimes hypertension, and occasionally there is a need for mechanical ventilation or administration of vasopressors, but that is rare.

After injection, the embryos are put back into a cell culture dish, where they divide and develop; after 30 hours or so, the hatched larvae look recognizably fishy under a microscope.

It has been reported previously however that, upon intravenous injection, CD4 + T cells swiftly migrate into LNs and a majority of them reside within the LNs for only about 12 h (Mandl JN et al..

Eight weeks postinjection, the engraftment was confirmed by flow cytometric staining of peripheral blood cells for human CD45 + / CD33 + cells, followed by randomization into study groups and treatment with a single bolus IV injection of IMGN632 or the control ADC.