Sentences with phrase «cell isolation»
Cell isolation refers to the process of separating and extracting specific cells from a mixture or a larger sample. Full definition
Target cells are captured through magnetic cell isolation workflow.
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The United Kingdom, Israel, and Sweden are among the few countries where stem cell isolation from superfluous in - vitro - fertilized embryos is allowed.
For Western blot assay and immunoprecipitation analysis, CD4 + T cells were isolated from mice spleen on day 21 after transplantation using a Mouse CD4 + T Cell Isolation Kit (Miltenyi Biotec), and the procedures were performed as described previously (35).
The work on stem cell isolation strategies for future clinical application was recognized with a Medical Technology Innovation award and the Overall Grand Prix Award at the Engineer Technology and Innovation Awards in 2010.
To do this I will combine novel cell isolation techniques with high - throughput sorting and cutting - edge sequencing technologies.
Technical knowledge of scientific disciplines; Performs mononuclear cell isolation from biological samples, this includes ficoll purification, counting and...
Phase 2.1 — In Vitro Revival: Primordial Germ Cell isolation, Culturing, and CRISPR / Cas9 Genome Editing
They will not be able to continue the cloning and stem cell isolation procedures unless a new license is issued.
Performed cell isolation, enumeration, and staining on organs and blood, including those of HIV - exposed mice
Recent reports have found that conventional cell isolation and genome amplification strategies, even using MDA, can yield as many as a million false positive single - nucleotide variants in a human genome, swamping the true positives (1).
To do that as gently as possible, Qiagen's QIAscout cell isolation system spreads cells across an array of magnetic rafts.
Dr. Joerg - Matthias Pollok, Head of the Laboratory for Tissue Engineering and Cell Transplantation, Department of Hepatobiliary and Transplantation Surgery at the University Medical Center in Hamburg, Germany explains, «Currently isolated liver cells are used for liver cell transplantation, but these cells suffer during cell isolation and cryopreservation, which is one reason there is limited success with this type of transplant procedure.»
We continue to prosecute new multidisciplinary strategies for nanodelivery of agents for skeletal and wound repair, skeletal cell isolation and characterization as well as development of translational programs for large scale musculoskeletal regeneration.
In addition to our original line of haematology products, we also offer primary cells and a comprehensive suite of immune cell isolation tools and instruments.
Overall stem cell colony formation efficiency following single cell isolation and flow cytometry was still quite low (∼ 0.5 %) despite the use of the Rho - associated kinase inhibitor but consistent results were obtained.
Naïve CD4 + T cells were isolated by MACS microbead negative sorting using the naïve T cell isolation kit (Miltenyi Biotec).
Careful sample handling is an essential requirement of many single - cell isolation strategies, a family of methods that has grown exponentially in recent years.
The researchers also anticipate that their gene expression data and newly described islet - cell isolation technique, coupled with the ongoing tissue procurement effort, will be helpful to others studying pancreatic development and diabetes.
CD4 + naive T cells were purified using the CD4 + naive T cell isolation kit (STEMCELL Technologies), according to the manufacturer's instruction.
Phase 2.1 — In Vitro Revival: Primordial Germ Cell isolation, Culturing, and CRISPR / Cas9 Genome Editing
Cells isolation procedures have to be performed at GMP conditions and viability and purity of the cells has to be determined.
In the United States, embryonic stem cell isolation is not permitted using federal funds; however, since August 2001, the Bush government accepted registration of 78 human embryonic stem cell lines on which research could be carried out using government finance.
Whole blood from rhesus macaques (Macaca mulatta) housed at the Tulane National Primate Research Center was used for CD4 + T cell isolation and ZFN treatment.
Study Plans for hematopoietic stem cell isolation and transduction with viral vectors, mouse conditioning, transplantation, manipulation and clinical signs monitoring are optimized and applied to the GLP studies.
Cell isolation is an important, but often overlooked, first step on the road to scientific discovery.
Cells isolation and delivery methods are of the highest importance in developing stem cells applications for clinical use.
Dr. Hackam's team has developed techniques of intestinal stem cell isolation and culture, and are working with tissue engineers and chemists towards the development of an artificial intestine, which has been tested in large and small pre-clinical models.
To advance the next generation of scientists, the gift provides ongoing support for the center's state ‐ of ‐ the ‐ art core facilities in imaging, therapeutic screening, flow cytometry and stem cell isolation and culture.