Responsible for assisting senior engineers in carrying out
cell level validation, testing and communicating results to applications staff
Not exact matches
The
level of fluorescence you saw in the
validation assay should give an accurate indication of how many pups will contain a mutation and many of the mice with the mutation will be homozygous for it in every
cell; however, if the microinjection was done as the pronuclei were fusing, you may have several mosaic mice.
The overall score encompass several factors: reproducibility of the antibody staining in different
cell lines (and if signal strength correlates with RNA expression
levels); assays for enhanced antibody
validation by using antibodies binding to different epitopes on the same target protein (independent antibodies), by knockdown / knockout of the target protein (genetic methods) and by matching of the signal with a GFP - tagged protein (recombinant expression); experimental evidence for location described in literature.