Sentences with phrase «cell line experiments»

Many scientific journals require researchers who report results based on cell line experiments to use DNA profiling to establish the identity of the used cells.

Not exact matches

The researchers experimented with inducing oxidative stress in a human cell line culture with and without VCOP (virgin coconut oil polyphenols) to observe how VCOP positively promoted catalase, a very important enzyme in protecting the cell from oxidative damage, and glutathione (GSH), a self - recycling antioxidant produced by the liver.

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Despite the presumed virulence of the strain — experiments with mouse lungs showed it produces 1000 times more bacteria in infected cells than do standard varieties — Valway says the number of TB cases that developed were kept in line with other typical outbreaks, which «shows that doing good contact investigations is important and preventative therapy works.»

That variability among cells containing the same program kicked off a whole new line of experiments that Elowitz says are focused on «what it is that is making different cells do different things.»

In experiments conducted on human lung endothelial cells and in mice, the researchers showed that NS1 caused permeability of the endothelium, which lines the walls of blood and lymph vessels.

Researchers are also experimenting with another form of immunotherapy called adoptive cell transfer, which harvests and then reintroduces patients» tumor - infiltrating lymphocytes — cells with anti-tumor capabilities that dig deep behind enemy lines.

Further experiments showed that each iPS cell line had a different pattern of DNA methylation, a molecular coating of the DNA that turns genes on or off in the cell.

The dispute had to do with Mikovits's insistence that another scientist at WPI had improperly ordered a cell line for experiments that were beyond the purview of his authority.

Several different experiments localized the active gene and its protein to stem cells buried within the crypts, or deep grooves in the intestinal lining.

His experiments revealed the cells that became rhabdomyosarcoma were not muscle cells, but were immature cells that would mature into cells lining the inner surface of blood vessels.

In experiments with muscle cells from the lining of blood vessels, the researchers found that a sudden mechanical force flips a genetic switch in these cells, making them more likely to contract.

To validate their computer modeling predictions, researchers performed experiments in human cancer cell lines, mouse liver samples and primary human hepatocytes.

The final guidelines on research with human embryonic stem cells issued on Monday by the National Institutes of Health set out criteria for determining which ES cell lines can be used in federally funded experiments and give NIH discretion to approve old lines that don't meet stringent modern ethical requirements.

Mindful of public sensitivities, Daley opted to pursue experiments using what he considers the least controversial human materials to create new nonpresidential stem cell lines — poor quality embryos and oocytes that, in his words, «otherwise would have been disposed of as medical waste.»

To put the combinatorial screen with the CLOUD to the test, Kubicek's group investigated the effect of pairwise combinations of CLOUD compounds on the viability on KBM7 leukemia cells, a cell line well suited for drug experiments.

Provide example data from experiments using PD - L1 positive tumors and a cancer cell - line engraftment into a humanized mouse model that was used to test anti-PD1 immune therapy

To test their hypothesis, the researchers carried out experiments on human melanoma cells, a line of cancer cells they chose for their ability to grow easily and quickly.

In experiments with cancer cell lines, the PIM1 inhibitors killed cells in a MYC - dependent manner, and in two different mouse models — one in which mice were implanted with patient tumors and the other in which a genetic alteration of MYC predisposes the mice to tumor development — the administration of PIM1 inhibitors resulted in significant tumor regression.

Their experiment has now shown that the gills of jawed vertebrates emerge from the same internal lining cells as their jawless relatives.

In preclinical experiments using human prostate cancer cell lines, Fu's team showed that increased PLK1 expression activated an oncogene known as c - RAF, which has previously been shown to play a role in regulating cell growth and division.

But its most immediate use could be to flag mislabeled or contaminated cell lines in cancer experiments, a major reason that studies are later invalidated.

In lab experiments, the research team used cell lines derived from 40 patient tumour samples to identify that CD151 contributes to the survival of cells of high - grade serous ovarian cancer origin.

Because the dataset now will be available to the scientific community, researchers will be able to account for these differences when designing and interpreting experiments using this HeLa cell line.

Biochemist Nirenberg of the National Institutes of Health gave a seminar describing a groundbreaking experiment in which he and a colleague had discovered how the cell interprets messenger RNA — by reading one triplet, or codon, of nucleotide bases at a time — to line up the amino acids that form proteins.

His team have begun recolonising the primate scaffolds with human cells that line blood vessels, the first step towards human - scale biolimb development, and have started experiments using human myoblasts in rats instead of the mice ones.

That's because experiments conducted on the International Space Station involving cells that line the inner surfaces of blood vessels (endothelial cells) show that microgravity accelerates cardiovascular disease and the biological aging of these cells.

Roein - Peikar also noted that their experiments are mainly based on Chinese Ovary Hamster cells, a cell line derived from the ovary of the Chinese hamster, often used in biological and medical research.

The goal of the first experiment was to see whether lncRNAs are differentially expressed in prostate cancer by measuring total RNA from prostate cancer cell lines and normal epithelial prostatic cells using NCode human ncRNA array and SurePrint G3 human lncRNA microarrays.

Its first target was the regulatory network involved in controlling the differentiation of THP - 1 cells, a line of human leukemia cells used in laboratory experiments.

These results were reproduced in three experiments with the three cell lines.

In another experiment, blocking the activity of inflammatory cytokines in the presence of DENV NS1 did not prevent disruption of human cell layers similar to those found in the lining of blood vessels.

Number of experiments: n = 15 (XpdTTD / TTD), n = 6 (XpdTTD / KO), n = 4 (XpdTTD / † XPCS), n = 2 (XpdTTD / † XP); 1 — 2 cell lines per genotype were included in each experiment.

Survival curves represent an average of four independent experiments; 1 — 2 cell lines per genotype were included in each experiment.

One of the many payoffs of the International HapMap Project was that it characterized genetic variation in fibroblast cell lines that could be ordered from Coriell for subsequent experiments.

The p - value indicates minimum significant difference between wt and the indicated cell lines analysed on the same microscopic slide within one experiment.

• ES cell line purity verification In addition to mouse strain purity verification, the facility provides genetic screening of ES cell lines used for gene targeting experiments.

In our experiments, both HNF4α and ASGPR1 are well - expressed in enhanced hiPS - HEP cells from all three hiPS cell lines (Figure 2), as shown by homogeneous nuclear staining for HNF4α and homogeneous membrane staining for ASGPR1.

Three independent experiments were performed to derive the average CFE and standard deviation per cell line.

The majority of gene - targeting experiments in mice are performed in 129Sv - derived embryonic stem (ES) cell lines, which are generally considered to be more reliable at colonizing the germ line than ES cells derived from other strains.

Wolin and her colleagues had begun to answer questions about Ro's function when their earlier experiments demonstrated that the Ro protein binds to small RNA molecules that are misfolded versions of those that help make up the cell's ribosomes, which are its protein assembly lines.

These cell lines offer convenient tools for experiments where transgenes and selection are required.

Hence, its members favor primary cell lines and try to avoid experiments with cultured cell lines, they favor three - dimensional cell cultures over two - dimensional cell monolayers that are cultivated on hard and flat surfaces and they try to maintain the three - dimensional context of plants, cell clusters, tissues sections and small animal embryos.

In a series of lab experiments with cell lines, human xenograft tumors in mice and primary human prostate cancer samples, the researchers demonstrated that miR - 34a inhibits prostate cancer stem cells by suppressing CD44.

We established several cell lineages from three independent transfection experiments and chose three lines (iPSC1, iPSC2, iPSC3) for further characterization.

At the end of the experiment, counting the numbers of each barcode serves as a readout that reveals how many cells from each cell line survived exposure to the compound.

T - DDOGs also successfully regulated expression of channelrhodopsin - 2, commonly used in optogenetics, opening up the possibility of adapting GFP lines for optogenetics experiments in specific cell populations.

This information is standard for all newly created cell lines and will help researchers decide which lines may be best for their experiments.

Researchers can also find information on the cancer cell lines that are most suitable for their experiments.

The number of cell lines can also vary depending on the experiment being implemented; one could test a handful of cell lines, or hundreds of them.