Sentences with phrase «cell line with»

We have used a patient cybrid cell line with a single point mutation in the overlap region of the ATP8 and ATP6 genes of the human mitochondrial genome.

Overall, making a human cell line with a gene knockout was straightforward, and is a testament to how far genome engineering has come.

For more on human embryonic stem cells, see Teisha Rowland's «All Things Stem Cell post on «Induced Pluripotent Stem Cells: A New Stem Cell Line with a Long History,» The National Institute of Health's Stem Cell FAQs, or, for a visual explanation of terms used, see All Things Stem Cell's Visual Stem Cell Glossary.

Gesicles are made by transfecting a 293T - based producer cell line with a vector containing an sgRNA for your target locus and our gesicle packaging mix, which contains everything you need in an optimized format.

Then, they incubated each cell line with a helping of either mildly toxic thiacloprid or super toxic imidacloprid.

Changes in replication, nuclear location, and expression of the Igh locus after fusion of a Pre-B cell line with a T cell line.

Every cell line with high Vimentin and no E-cadherin had no detectable miR - 200.

Thus, high ALKBH1 activity can globally decrease the amount of proteins made in a cell, as the researchers observed in a human cell line with abnormally high levels of ALKBH1.

WPI fired Mikovits on 29 September because it said she refused to share a cell line with another researcher there.

A new stem cell line with the mutation for fragile X syndrome may help uncover the mechanism behind the debilitating disorder

Professor Ali Tavassoli, who led the study with colleague Dr. Ishna Mistry, explains: «In an effort to better understand the role of HIF - 1 in cancer, and to demonstrate the potential for inhibiting this protein in cancer therapy, we engineered a human cell line with an additional genetic circuit that produces the HIF - 1 inhibiting molecule when placed in a hypoxic environment.

Before we established in - house human taste cell (HTC) technologies, it was not possible to use human tongue - derived cells as a model, due to the lack of homogenous, proliferating cell lines with defined properties, which is a prerequisite to establish comprehensive research and screening programs.

The work, funded by the US National Human Genome Research Institute, aims to create human cell lines with subtly different genomes in order to test ideas about which mutations cause disease and how.

This technique is able to distinguish cancer cell lines with differing metabolic activities and reveals heterogeneous uptake patterns in neurons, mouse brain tissues and tumor tissues with clear cell - to - cell variations.

With the help of my colleagues I started growing retroviral vector - containing plasmids in bacterial cultures, isolating the plasmid DNA and transfecting special producer cell lines with these plasmids.

They will share their cell lines with other researchers via the Prechter Repository at U-M.

«Marie and her colleagues helped us genetically compare the cell lines with each other.

Using a very sensitive DNA analysis technique that can also be employed when only very small amounts of DNA from old tissue are available, the researchers could compare the two current cell lines with the tumor from which the cell line was established.

Earlier this year, researchers in Germany published a scientific paper that described the first sequence of the full HeLa genome, comparing the DNA of HeLa cell lines with that of cells from healthy human tissues.

To find out, Barton and colleagues treated cell lines with the drug enzalutamide and with shRNAs designed to knock down androgen receptor levels.

Last week, Hwang filed a complaint with the Seoul prosecutor's office claiming that someone had switched his stem cell lines with ones from MizMedi.

NeuroStemcell is focused on the identification and systematic comparison of progenitor cell lines with the most favourable characteristics for mesDA and striatal GABAergic neuronal differentiation, generated either directly from human embryonic stem (ES) cells, from Neural Stem (NS) cells derived from ES cells or fetal brain, from induced Pluripotent Stem (iPS) cells or from in vitro short - term expanded neural progenitors from ventral midbrain grown as neurospheres (VMN, Ventral Midbrain Neurospheres) 4, and perform rigorous and systematic testing of the most prominent candidate cells in appropriate animals models.

Treatment of lung cancer cell lines with mebendazole caused mitotic arrest by depolymerization of tubulin, followed by apoptotic cell death.

Treatment of the Karpas 299 and SU - DHL1 cell lines with increasing concentrations of rapamycin, an inhibitor of the mTOR - raptor complex, resulted in a marked concentration - dependent decrease of the phosphorylation levels of mTOR, p70S6K, 4E - BP1, and total eIF4E (Fig. 4A).

To investigate if AKT activation is sufficient to activate the mTOR pathway in ALK + ALCL cells, we infected the Karpas 299 and SU - DHL1 cell lines with an adenoviral vector, adeno - myrAkt, expressing constitutively active AKT (23).

Infection of both cell lines with adeno - myrAkt resulted in substantially increased of Ser473p - AKT levels (A) associated with increased phosphorylation (activation) of mTOR, p70S6K, and rpS6 (B).

Of note, treatment of ALCL cell lines with rapamycin also resulted in a slight decrease of Ser473 phosphorylation of AKT.

The MDA - MB - 231 — derived 1833 cell line, which is characterized by enhanced bone - specific metastasis compared to the parental cell line or cell lines with increased tropism to lung and brain, showed increased abundance of ABL1 and ABL2 (fig.

When Possemato suppressed PHGDH protein production in breast cancer cell lines with elevated levels of it, the cells stopped proliferating.

Nevertheless, cell lines with varying differentiation potential might also aid in study of molecular regulation of differentiation processes.

In the first step, stainings in different cell lines with the same antibody are reviewed and the results are compared with available protein / gene characterization data for subcellular location.

To directly evaluate the relationship between ABL family kinases and metastasis, we analyzed ABL1 and ABL2 protein abundance in MDA - MB - 231 — derived breast cancer cell lines with different organ metastasis tropisms (19).

According to the researchers, when expression of the gene that codes for this protein — phosphoglycerate dehydrogenase or PHGDH — is suppressed in tumors and cell lines with an overabundance of the protein, the rate of cellular growth declines markedly.

We use this in vitro model of spontaneous tumorigenesis to generate a library of cell lines with 25 different de novo JAK1 - activating mutations, 6 of which are targeting two residues, Phe958 and Pro960, located in the hinge region at the periphery of the ATP - binding pocket.

Isolation of amniotic stem cell lines with potential for therapy.

In this study, published in Stem Cell Reports, the researchers used both murine and human pluripotent stem cell lines with airway secretory lineage reporters, which enables their tracking, purification and profiling.

The Mutant Mouse Resource and Research Center (MMRRC) distributes and cryopreserves scientifically valuable, genetically engineered mouse strains and mouse ES cell lines with...

Not exact matches

With a virtual office you can have many different number all connecting back to a single office line or cell phone.

The majority of study participants reported that it's all good, fine and kosher with them (well, technically «generally OK») to use a cell phone when walking down the street, riding public transit and waiting in line.

Finally, have some sacrosanct times when you're with your kids or on vacation when you turn your work phone off, but can be reached in emergencies by land line or personal cell, (the way things were before mobile work phones became ubiquitous).

Even phone systems now enable remote working, with land lines capable of forwarding to your cell phone, anytime, anywhere.

One way to enhance eggs, developed by the company OvaScience, involves supplementing an egg with mitochondria taken from stem cells found in the lining of a woman's uterus.

In diseases such as malaria and sickle cell disease, red blood cells break down, with harmful effects on the rest of the body — particularly the lining of small blood vessels.

Tesla in late 2016 shared plans for a line of glass roofing tiles with integrated solar cells.

As a cancer researcher, do you think the mechanisms of tumor growth are somehow changing to come into line with your perceptions, or is it possible that the process of our learning more about DNA mutations and cell architecture and nutrient exchange and epigenetic effects make it possible for us to inch ever closer to understanding that which is already going on under our noses?

In all likelihood, soon after he is sworn in, federal dollars will start flowing toward research on new embryonic stem - cell lines, bringing with it new battles to fight.

Not surprisingly, President Obama received high marks from the journal, largely because his response to the question of whether he would lift Bush's ban on the federal funding of new embryonic stem - cell lines is in line with the view of most in the scientific community.

The bottom line and then I'll shut up: in a healthy conversation, with no one trying to WIN THE ARGUMENT, this cartoon should be both cell colors freely mixing with each other — not «converting» or merging with one another.

Duly reverential, the panel satisfied itself with simply listing all the research possibilities, including the improvement and increased safety of IVF, the creation of cell lines that might someday be useful for bone marrow transplantation, repair of spinal cord injuries, skin replacement and, naturally, the hint of a greater understanding of cancer.»

Advocates of embryonic stem cell research were thought to be in a long line of pioneers fighting the restraints of religious doctrine to push forward with scientific research.