Sentences with phrase «cell lines from»

The San Diego Zoo has its parallel Frozen Zoo, an archive of thousands of DNA samples and cell lines from a host of species.

Gerber H, Peter HJ, Bösiger J, Studer H, Drews R, Ferguson DC, Peterson ME, Becker DV: Different continuous cell lines from adenomatous feline goiters widely differ in morphologic, functional and growth parameters.

Over five years it will derive three induced pluripotent stem - cell lines from skin and blood samples taken from 500 patients that it hopes to enrol in the project.

In addition, three California - based ALS research labs have joined forces to form the Neuro Collaborative, which will create induced pluripotent stem (iPS) cell lines from ALS patients that can be used to screen for new drugs and will be shared with the other groups.

Some receptors display a broad pattern of expression across tumour types, while others were expressed in cell lines from a particular tissue.

The MMRRC currently holds 24 mouse models and several ES cell lines from this collection and the MMRRC at UC Davis will generate an additional group of mouse models for this collection from the supplied ES cells.

To further maximize accuracy, the researchers recommend that future studies involving stem cells use cell lines from as many individuals as possible rather than generating multiple cell lines from the same individual.

Therefore, it is necessary to establish multiple independent iPS cell lines from individual donors to ensure efficient generation of various types of functional cells for future application in the treatment of diseases.

«We have already received four remote tumor samples and generated two new ROS1 cell lines from patients who never even had to travel to University of Colorado,» says Doebele.

The laboratory, in the project I - Stem, received the first approval by the Biomedicine Agency, for importation into France of hES cell lines from abroad in accordance with current legislative framework.

DBA Cord Blood Registry (CBR) are collaborating to customize the creation of high - quality stem cell lines from umbilical cord blood tissue...

In this study, we established mouse iPS cell lines from NPCs of neonatal EGFP - transgenic mice on the HFF feeder cells via viral transduction of 4 factors, combined with application of 2i.

Based on mRNA expression data, two suitable cell lines from the cell line panel are selected for the immunofluorescent analysis of each protein.

Whereas the derivation of stem cell lines from human embryos is subject to specific regulation by the Human Fertilisation and Embryology Authority (HFEA), UK law does not distinguish between fetal tissue and other tissue from living patients.

The team that generated the insulin - producing embryonic stem cell line, e.g., had a success rate of under six percent, using 71 eggs to produce four stem cell lines from the embryos they created and destroyed.

The researchers established cell lines from the original egg and in seven out of eight cases the amount of mutated mitochondrial DNA remained low when the cells were grown in the laboratory.

In studies of cell lines from more than 20 Friedreich's patients, the molecular prosthesis restored expression of the frataxin protein.

In effect, the new guidelines provide an incentive to private research entities to obtain so - called «leftover» embryos from fertility clinics and derive stem cell lines from them in order to obtain NIH research dollars to study the derived lines.

Until now, scientists examining the causes and effects of insulin resistance have struggled with a general lack of human cell lines from tissues such as muscle, fat and liver that respond significantly to insulin, Kahn says.

In parallel, we have generated iPS cell lines from fibroblasts of two patients (Coriell) suffering from Leber Congenital Amaurosis (LCA).

We purchased MDA - MB - 231 (MB231) and MCF7 breast cancer cell lines from American Type Culture Collection.

The selection strategy targeted cell populations expressing the inducible endothelial cell adhesion molecules, E-selectin and VCAM - 1, and proved successful in generating microvascular endothelial cell lines from a number of different organs.

Using a new TaqMan PCR - based technique, the researchers screened a number of cancer cell lines from various tissues, and discovered that tRNA halves were specifically expressed in large quantities in sex hormone - dependent cancers, i.e., estrogen receptor (ER)- positive breast cancer and androgen receptor (AR)- positive prostate cancer that are driven by the hormones estrogen and testosterone.

Tissue - specific microvascular endothelial cell lines from H - 2K (b)- tsA58 mice for studies of angiogenesis and metastasis

We capitalized on one of the innate responses of microvascular endothelial cells, i.e., the up - regulation of cell adhesion molecules in response to inflammatory agents, to generate endothelial cell lines from different organs.

Since last year, teams in Italy, Scotland, and the United States have reported deriving ES cell lines from human parthenotes, although none of the lines is well studied.

Following up on clues — including the fact that PMS is 56 percent heritable — the NIH researchers studied the genetic control of gene expression in cultured white blood cell lines from women with PMDD and controls.

The study was conducted on 60 lymphoblastoid cell lines from female patients, most of whom had a family history of mental illness.

Melton says his lab has iPS cell lines from people with diabetes — both type 1 and type 2, in which the β cells are not destroyed — and healthy controls.

Furthermore, stem - cell lines from mice begin to lose their ability to produce many types of cells after as few as 20 generations, and researchers assume the same will prove true for stem cells from humans.

Australian researchers have so far generated over 50 embryonic stem cell lines from surplus embryos, and three research groups have been licensed to attempt somatic cell nuclear transfer.

Researchers at Columbia University and the New York Genome Center have developed a method to quickly and accurately identify people and cell lines from their DNA.

On the other hand, the problem is, you know, with embryonic stem cells, they haven't been able to get stem cell lines from livestock animals that can proliferate in that way, without just sort of veering up in their own direction and turning into, instead of muscle, turning into brain tissue or bone tissue or something else.

At the University of Washington, Hannele Ruohola - Baker, professor of biochemistry and associate director of the Institute for Stem Cell and Regenerative Medicine, Julie Mathieu, acting instructor and Henrik Sperber, graduate student, withdraw embryonic stem cell lines from liquid nitrogen storage.

Cell lines from human tumours did exist, but were considered unsafe: what if cancerous cells were transferred along with the vaccine?

Dr. Jochen Maurer and his research group were able to cultivate several cancer stem cell lines from triple receptor - negative breast cancer that are excellent representations of the original tumors they isolated from the patients.

The team produced 11 stem cell lines from 11 men, women, and children with conditions such as diabetes, spinal cord injury, and inherited immune deficiency.

In order to thoroughly investigate cures for a diverse population, cell lines from a large sampling of individuals are needed.

Potti and his colleagues began by testing chemotherapy drugs on cultured cell lines from human tumors, such as from the lung, breast, or ovary.

In 2007, however, scientists at International Stem Cell, a California - based biotech firm, reported the first successful creation of human stem cell lines from unfertilized eggs.

Subsequent efforts have averaged one stem cell line from 16.8 embryos.

«We can work with any embryonic stem cell line from any source and are not restricted to working with the very small number of federally approved lines as is the case for researchers in the United States,» says Minger.

The paper not only seemed to validate the group's claim a year earlier that it had created a single cell line from a cloned human embryo, but it also reported a huge increase in efficiency for the technique.

Although scientists have welcomed the development, they say the real breakthrough will be when someone manages to extract ES cells from the inner cell mass of cloned blastocysts and generate a cell line from them.

One idea is to grow a cell line from a single cell removed from an early embryo, leaving the rest of the embryo intact.

The team makes it clear that it thinks its approach is superior to one reported last month in Nature by scientists at Advanced Cell Technology (ACT) in California — deriving an ES cell line from a single cell taken from a morula (ScienceNOW 23 August).

In 2008 a group of medical researchers led by Robert Lanza at Advanced Cell Technology in Worcester, Massachusetts, reported another leap: They discovered a way to avoid destroying the embryo by deriving an entire stem cell line from a single embryonic cell.

He also inserted the disease - causing variation into one healthy cell line from a family member, as well as the cell line from the unrelated control.

We found that the U87MG cell line from ATCC had a different DNA profile than the original cell line in Uppsala», says Bengt Westermark.

«The comparison showed that the Uppsala cell line was genetically identical with the original tumor whereas the U87MG cell line from ATCC had a different, unknown origin.