Sentences with phrase «cell lines growing»

As important genes are identified for de-extinction, the researchers can attempt to edit them into the Woolly Mammoth cell lines growing at the Church Lab.

The majority of cell lines growing at the permissive temperature (33 °C) underwent an initial population doubling within 48 h.

All human cell lines growing in tissue culture dishes, and about 95 % of cells in a human being, split evenly, resulting in daughter cells of equal size.

Capsaicin additionally produced a significant deceleration of the development of prostate tumors created simply by those human cell lines grown in mouse models.

By functionally linking the signal transduction of melanopsin to the control circuit of the nuclear factor of activated T cells, we have designed a synthetic signaling cascade enabling light - inducible transgene expression in different cell lines grown in culture or bioreactors or implanted into mice.

Researchers believe it is the largest epigenetic study yet for any single cancer type and, importantly, the first to use a large cohort of primary patient tumor tissues instead of cell lines grown in the laboratory.

It also works in cells taken directly from human patients, which are usually much more difficult to manipulate than human cell lines grown specifically for lab research.

From a biologist's standpoint, this is a really unique thing, as we don't usually know who is behind the cell lines we grow in the lab.

Stem cell lines grown in the lab provide scientists with the opportunity to «engineer» them for use in transplantation or treatment of diseases.

Not exact matches

Where is the clear line in a progression from (1) using animal insulin to treat diabetes, to (2) using gene remodeling techniques to grow insulin in a host bacterium that will reproduce rapidly and from which a plentiful supply of insulin can be harvested, to (3) genetic surgery to replace the defective gene in a person diagnosed as diabetic, to (4) genetic surgery immediately after fertilization in order to replace the defective gene and alter the germ cells which would otherwise have transmitted the disease to one's offspring?

Newborns typically have loose junctures between the cells of the stomach lining, so during the first few months of life, these junctures grow tighter as your baby's protective tummy barrier matures.

Endometriosis occurs in women when cells from the lining of the womb grown in other areas of the body.

If the egg gets to the uterus and is fertilized by a sperm cell, it may plant itself in that lining and grow into a baby.

As well as allowing the use of stem cells grown from established cell lines, the technology could enable the creation of improved human tissue models for drug testing and potentially even purpose - built replacement organs.

For instance, a growing number of households no longer have land lines, but rely on their cell phones instead.

Introducing human prostate cancer cell lines into mice, Wu and his colleagues saw a particular enzyme called MAOA activate a cascade of signals that made it easier for tumor cells to invade and grow in bone.

To create the effect of tobacco smoke on cells, Vaz, Baylin and their colleagues began their studies with human bronchial cells, which line the airways of the lungs, and grew them in a laboratory.

In addition, where cells derived from embryonic stem cells are great at proliferating — a potentially critical feature if one wants to grow sufficient numbers of cells for clinical use — ones from the iPS lines were much feebler.

Doing this in bacteria or cell lines used for growing chemicals would be of huge importance to industry, where viral infections can shut down entire production lines.

In a new study published in the journal Development, the KU Leuven researchers show that individual or small groups of cells from uterus biopsies can be made to grow into three - dimensional structures that show many of the features of the womb lining, including the ability to produce mucus.

Finally, the team grew the tissue in a three - dimensional system, which coaxed it into forming all the structures found in gut tissue, including the finger - like villi that protrude inwards to increase surface area, and recesses called crypts containing the intestinal stem cells that renew the gut lining weekly (Nature, DOI: 10.1038 / nature09691).

As other researchers asked for samples, the cells replicated, grew, and proliferated so wildly that they often took over and wiped out cell lines of any kind with which they happened to come in contact.

«We believe an overwhelming number of these altered cells reach the lining of the abdominal cavity, survive and grow,» Bulun said.

He and two colleagues then used spleen cells from the animal to grow 43 cell lines.

The bacteria are full of shiny white globules of sulphur and grow in long lines of single cells, making them look like a string of pearls.

With the help of my colleagues I started growing retroviral vector - containing plasmids in bacterial cultures, isolating the plasmid DNA and transfecting special producer cell lines with these plasmids.

Now, researchers in Europe report they can get new lines from «arrested» embryos — early embryos that have ceased to develop but that contain individual cells that can be induced to grow separately.

Part of the trouble is the ingredients: Subtle variations in tissue - culture chemicals and Matrigel, or in different stem cell lines and how they are grown first in 2 - D culture, can have a big impact on how the organoids turn out, Novitch says.

One idea is to grow a cell line from a single cell removed from an early embryo, leaving the rest of the embryo intact.

If it is blocked, the cancers in cell line studies and in animals just stop growing — which is really quite striking.»

It performs this critical service in embryonic development, growing organisms and in a few specialized adult cell lines, including stem cells.

To test their hypothesis, the researchers carried out experiments on human melanoma cells, a line of cancer cells they chose for their ability to grow easily and quickly.

In studies of laboratory - grown human tumor cell lines, the drug disrupted tumor cell division and prevented growth of advanced cancer cells.

To do this, they switched from using dead tumour cell samples to patient - derived tumour cell lines, in which fresh samples of a person's tumour are grafted onto mice and grown to the required volumes.

Scientists have already reported progress in growing precursor cells for eggs and sperm from both iPS and embryonic stem cell lines.

The study used a well - known line of pancreatic cancer cells (AsPC - 1) in the laboratory and assessed how well this grew when treated with either the chemotherapy drug gemcitabine or different levels of commercially available chokeberry extract alone, and when treated with a combination of gemcitabine and chokeberry extract.

Malaria parasites grow in red blood cells and stick to the endothelial lining of blood vessels through a large family of parasite proteins called PfEMP1.

The group reported growing multiple parthenogenetic embryonic stem cell lines by incubating eggs in a warm, low - oxygen culture medium.

To find methods for stemming the tide of liver - damaging microbes, Schnabl and team tried experimentally bumping up copies of the REG3G gene in intestinal lining cells grown in the lab.

The virus, however, grew efficiently in a human A549 lung adenocarcinoma cell line.

For the time course study, cells were treated with 20 μM of EGCG for 12, 24, 48, 72, or 144 h. Human colon cancer cell line HT - 29 and prostate cancer cell line PC3 were obtained from American Type Culture Collection (Manassas, VA), and were grown in McCoy's 5A and RPMI 1640 containing 10 % fetal bovine serum, respectively.

NeuroStemcell is focused on the identification and systematic comparison of progenitor cell lines with the most favourable characteristics for mesDA and striatal GABAergic neuronal differentiation, generated either directly from human embryonic stem (ES) cells, from Neural Stem (NS) cells derived from ES cells or fetal brain, from induced Pluripotent Stem (iPS) cells or from in vitro short - term expanded neural progenitors from ventral midbrain grown as neurospheres (VMN, Ventral Midbrain Neurospheres) 4, and perform rigorous and systematic testing of the most prominent candidate cells in appropriate animals models.

NCI's efforts to develop new laboratory models of human cancer includes vastly increasing the number of human cancer cell lines (grown as two - dimensional and three - dimensional cultures) and patient - derived tumor xenografts.

The Broad Cancer Dependency Map team adds CRISPR - based data from 342 cancer cell lines to their growing catalog of genetic dependencies in cancer, and a new method for ensuring that data's accuracy.

The Joslin team found that intestinal tumors grow just as quickly in a mouse cancer model whose «epithelial» cells lining the inside of the intestine have been genetically modified so that insulin can't activate the cells.

The researchers established cell lines from the original egg and in seven out of eight cases the amount of mutated mitochondrial DNA remained low when the cells were grown in the laboratory.

Cellartis human iPS cells from the ChiPSC22 line (Figure 2) and the ChiPSC18 line (Figures 3 — 7 and Table I) were grown in the Cellartis DEF ‑ CS 500 Culture System before editing.

For the first time since the linkurl: National Institutes of Health; http://www.nih.gov/ released its new guidelines for the derivation of human embryonic stem cell (hESC) lines last summer, a line approved under the Bush administration has been recommended for inclusion into the growing federal registry of lines eligible for federal funding.

It has not been grown in a T - cell line, only on human PBMCs

Our study shows that selecting the appropriate cell line, growing the cells as 3 - D aggregates and infecting them when they are fully differentiated is key for successful in vitro cell culturing of human noroviruses.»