Sentences with phrase «cell lines used»

Approximately 20 - 30 % of all cell lines used in biomedical research are contaminated.

Cell lines used in this study (HCC1806 and MX - 1) were both susceptible to treatment with the GHRH antagonist, MIA - 602.

In essence, the signers were trying to get the President to change his mind about stem cell lines used for research so that Congress does not have to create a legislative solution.

For the remaining genes, all genes detected at TPM > 1 in at least one of the tissues or cell lines used in the RNA - seq analysis are classified as «Evidence at transcript level».

As names are not even used consistently in publications and deviations from the original name are not rare, the situation increasingly causes confusion about the identity of cell lines used in specific studies.

• ES cell line purity verification In addition to mouse strain purity verification, the facility provides genetic screening of ES cell lines used for gene targeting experiments.

The three Group 3 medulloblastoma cell lines used in this study reinforced the observation, highlighting significant differences in epigenetic regulators at work in medulloblastoma cell lines versus tumor samples.

Doing this in bacteria or cell lines used for growing chemicals would be of huge importance to industry, where viral infections can shut down entire production lines.

Since then, Freeze's lab has created cell lines using samples collected from 10 NGLY1 patients, and he hopes the crowdsourcing campaign will yield testable new hypotheses.

Ongoing attempts to create human stem cell lines using SCNT have yet to achieve success.

Researchers have already developed dozens of patient - specific cell lines using this new method.

First, we produced PARP1 - KO ovarian cancer cell lines using CRISPR / Cas9 gene editing to test the loss of PARP - 1 as a resistance mechanism to all clinically used PARP inhibitors.

We have generated more than 10 knockout cell lines using homologous recombination and AAV - based system.

June 21, 2007: Bush vetoes another DeGette - sponsored bill that would allow research on all stem - cell lines using cells created for in - vitro fertilization and scheduled to be discarded.

Earlier this year the team announced that they had cataloged 769 strong genetic dependencies across 501 CCLE - curated cell lines using genome - scale RNAi screens — the fruits of a nearly 10 - year effort.

I and a colleague have attempted to use this on two different types of cell lines using Flow cytometry and fluorescence microscopy all of which proved unsuccessful.

Western blot analysis of Beta - actin in various tissues and cell lines using Proteintech antibody 60008 -1-Ig at a dilution of 1:5000.

The cell line used by Asterias, which was originally derived in the 1990s, is sufficient to supply ALL of the AST - OPC1 needed for clinical trials and for potential commercialization of the product following regulatory approval.

Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004 -1-Ig at a dilution of 1:10000.

Total RNA was isolated from each cell line using an RNA Extraction Kit (Qiagen Inc., Valencia, CA, USA) in accordance with the manufacturer's instructions.

Hon et al created a low - passage cancer cell line using a Her2 + ER - / PR - tumor from an East Indian female.

For microRNA (miRNA) analysis, total RNAs, including miRNAs, were isolated from the cell lines using TRIzol reagent (Invitrogen Corporation) in accordance with the manufacturer's instructions.

The technical evaluation of projects may require the provision of additional data such as information on the genetic modification of your mutant mouse line if applicable (e.g. affected gene, MGI ID of the gene, type of mutation, ES - cell line used, genetic background (e.g. number of backcross generations), safety level, description of DNA modification, vector, remaining non-recipient DNA, donor organism), mutant phenotype (s), special housing or care requirements, current sanitary status, and intellectual property rights (who generated the mouse line, owner of the mouse line)

Others were disappointed that the White House had effectively prohibited the creation of new stem cell lines using federal funding.

That bill, based on one from Rep. Diana DeGette, D - Colo., would have allowed federal dollars to flow toward research on stem cell lines using embryos left over from in vitro fertilization, slated for disposal and donated by the parents.

We report the sequencing and assembly of a reference genome for the human GM12878 Utah / Ceph cell line using the MinION (Oxford Nanopore Technologies) nanopore sequencer.

This course is aimed at researchers who are familiar with basic molecular and cell biology techniques and want to learn how to create an engineered mammalian cell line using the most recent and advanced CRISPR / Cas9 system.

These results clearly indicate that analysis of future xenograft experiments should take careful note of the size of the xenografts as well as how hypoxia influences CSC in the specific cell line used.

NEs were prepared from various cell lines using a standard protocol (Dignam et al., 1983).

Not exact matches

The majority of study participants reported that it's all good, fine and kosher with them (well, technically «generally OK») to use a cell phone when walking down the street, riding public transit and waiting in line.

Phone surveys used to be the standard in the industry back when all customers had land lines, but in the era of the cell phone and do - not - call list, it's less reliable.

Memphis Meats acknowledges it used FBS to start its cell lines but says, «We have validated a production method that does not require the use of any serum, and we are developing additional methods as we speak.»

The cells coming off the line today will be used in Tesla's Powerwall 2 and Powerpack 2 energy products.

Single - celled organisms reproduce by mitosis, whereas multi-cellular organisms reproduce by meiosis, using specialised sex cells, the germ line.

In August of last year, President Bush approved the use of federal funds to support research on a limited number of existing human embryonic stem cell lines.

Where is the clear line in a progression from (1) using animal insulin to treat diabetes, to (2) using gene remodeling techniques to grow insulin in a host bacterium that will reproduce rapidly and from which a plentiful supply of insulin can be harvested, to (3) genetic surgery to replace the defective gene in a person diagnosed as diabetic, to (4) genetic surgery immediately after fertilization in order to replace the defective gene and alter the germ cells which would otherwise have transmitted the disease to one's offspring?

The second part of the automation job was the complete process data acquisition for the filling process cells that cover the data processing for seven filling lines, with approximately 150 associated controllers used to cyclically read 21,000 digital and 2,000 analogue data points.

Our bodies then use these essential nutrients to rebuild cells and particularly the lining of our intestine — which prevents those food sensitivities that seem to be on the rise these days.

Before we established in - house human taste cell (HTC) technologies, it was not possible to use human tongue - derived cells as a model, due to the lack of homogenous, proliferating cell lines with defined properties, which is a prerequisite to establish comprehensive research and screening programs.

While a monthly option is hosted, members are encouraged to use the on - line platform to create small cell groups for additional interest and geographic activities.

Older wireless receivers used analog signals, but these are open to interference from other wireless devices like cell phones, cordless land line phones, radios, etc..

(In my district, even cell phones and Facebook are sometimes used to bully kids in the «uncool» line.)

As well as allowing the use of stem cells grown from established cell lines, the technology could enable the creation of improved human tissue models for drug testing and potentially even purpose - built replacement organs.

When a cell divides, chromosomes line up with similar mates such that if one chromosome is scrambled, it can use its counterpart as a template for repair.

Using an experimental FA cell line, they also found that cells without frataxin produced lower levels of ApoA - I compared to controls.

That includes not only procedures in which embryonic - stem - cell lines are created, but also those that use previously derived cell lines.

Also, each was tested on a companion cell line already multi-drug resistant, as if the patient already had undergone chemotherapy using Paclitaxel.

To solve this, West proposed «therapeutic cloning» — taking the nucleus out of a patient's cell, transferring it into an egg cell to create a cloned embryo, then using that embryo to derive patient - matched stem - cell lines.

One perplexing problem, however, is that three out of 13 of the iPS cell lines created by Hochedlinger's team had a complete extra set of chromosomes, which has not been seen with iPS cells made using retroviruses.

Each of the four compounds was then tested in the lab to see how it would affect a line of prostate cancer cells relatively sensitive to the chemotherapeutic Paclitaxel, commonly used to treat prostate cancer patients.