Sentences with phrase «cell lysates»

Cell lysates refer to the contents of a cell that have been broken open or ruptured, releasing all the internal components. It is the liquid or solution obtained after destroying the cell membrane, allowing scientists to study and analyze the contents of the cell. Full definition
The SMN (human) Enzyme Immunometric Assay (EIA) kit is a complete kit for the quantitative determination of SMN protein in cell lysates of human origin.
Using mass spectrometry - based analysis and bioinformatics comparisons, the team identified 889 proteins in conditioned HMEC medium and compared these proteins to those identified in whole cell lysates and the serum added to the cell growth medium.
The Assay Designs ® SMN (human) Enzyme Immunometric Assay (EIA) kit is a complete kit for the quantitative determination of SMN protein in cell lysates of human origin.
Notably, analysis of cell lysates from ABL2 - depleted and ABL1 / ABL2 double - knockdown breast cancer cells showed a greater reduction of the phosphorylation of CrkL compared to cells with knockdown of ABL1 alone (Fig. 2K).
Total cell lysates of HuH - 7 cells were incubated with GST or GST - Sp1 fusion protein — prebound glutathione - Sepharose 4B beads and the bound fractions were subjected to immunoblotting with antibody against DDX3 (top).
The assay has been validated for use with human and mouse cell lysates and tissue homogenates, such as peripheral blood mononuclear cell (PBMC) lysates, and will be marketed through Enzo's worldwide sales and marketing group.
Antibody titers of positive control anti-sera and reactivity of pre-immune sera to polytropic MuLV - infected (upper panel) and uninfected (lower panel) HeLa cell crude cell lysates in WB testing.
The Heart for Companion Animal Research at Colorado State College has shown that cats vaccinated with FVRCP vaccines grown on Crandell - Rees Feline Kidney (CRFK) cell traces can develop antibodies to renal (kidney) proteins, and that cats hypersensitized to CRFK cell lysates can develop interstitial nephritis.
EdiGene Knockout Cell Lysates can be used to validate antibodies for specificity or used as high - quality negative controls for Western blot analysis.
The research, performed using recombinant protein from cell lysate isolated in vitro, also validates a methodology for characterizing large, multivalent membrane proteins in general.
Live cell assays constructed with genetically encoded, fluorescent biosensors can provide significant advantages over endpoint assays measured in cell lysates because functional information about the timing and location of cellular responses can be monitored in cells that are relevant to disease.
Plasma or serum samples were diluted 1:50 and reacted separately to 150 ug of infected and uninfected cell lysates overnight at 4 °C after protein separation through 4 - 12 % polyacrylamide gels and transfer to nytran membranes, as previously described [35, 36].
Western blot analysis of whole - cell lysates demonstrated that ospC7 no longer synthesized OspC (Fig. 3).
Anti-HIF-1-alpha unpurified antibody (ab51608) reactivity with reduced Hep3B cell lysate after transient transfection of scrambled siRNA (lanes1 - 3 and 7 - 9) or HIF -1-alpha siRNA (lanes 4 - 6 and 10 - 12).
Unpurified ab32518 used to immunoprecipitate IKB alpha from human HeLa whole cell lysate.
Surprisingly, we did not observe any increase in VCAM1 in either the TBS fraction or total cell lysates of brain homogenates (Fig 6C).
Cell lysates of hippocampal tissue from the aged huAPP / PS1 and control mice were analyzed for an effect of J147 on the APP processing pathway by immunoblotting with antibodies against BACE (D) and APP (E).
(I) Immunoblotting was performed using the indicated antibodies on whole - cell lysates from cells incubated or not with TRAIL.
Infected and uninfected HeLa crude cell lysates were prepared for WB testing as previously described [35].
Twelve hours after transfection, cells were treated with or without IFNα for another 24 h. Cell lysates (Input) were then immunoprecipitated (IP) with anti — IRF - 9 antibody followed by Western blot (WB) analysis with anti-STAT2 antibody.
The Center for Companion Animal Studies at Colorado State University has shown that cats vaccinated with FVRCP vaccines grown on Crandell - Rees Feline Kidney (CRFK) cell lines can develop antibodies to renal (kidney) proteins, and that cats hypersensitized to CRFK cell lysates can develop interstitial nephritis.
Luciferase expression can be quantified both in cell lysates and in live mice using a luminometer.
«When you prepare the cell lysate and [perform] DNA denaturation, it introduces an artifact called cytosine deamination that's due to the elevated temperature [step of the protocol],» says Joyce Peng, global marketing director for Novogene in Chula Vista, California.
Each lysate product is sold in kit format consisting of a KO cell lysate and a parental cell lysate, which are immediately available from OriGene for the academic and industrial research markets.
Derived mostly from human embryonic kidney 293T (HEK293T) and HeLa cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gene.
For immunoblot analysis, 50 μg protein from each total cell lysate was electrophoresed through 10 % SDS - polyacrylamide gels, then transferred to PVDF membranes (Bio-Rad).
Will immunoprecipitate from in vitro translation assays but not from cell lysates.
Lane 1: Wild - type HAP1 whole cell lysate (20 µg) Lane 2: IKB alpha knockout HAP1 whole cell lysate (20 µg) Lane 3: Hela whole cell lysate (20 µg)
ab32518 (purified) at 1/20 immunoprecipitating IKB alpha in HeLa cell lysate (Lane 1).
Unpurified ab32518 used to immunoprecipitate IKB alpha from rat PC12 whole cell lysate.
WB: Jurkat whole cell lysate.
Subcellular fractionation of cell lysates was prepared using the ProteoExtract Subcellular Proteome Extraction Kit (Calbiochem - EMD Biosciences, San Diego, CA, USA), and protein concentrations were determined by EZQ Protein Quantification Kit (Invitrogen Corporation).
Cell lysates of either hippocampal tissue or entorhinal cortex tissue from aged AD mice on control diet (AD Ctl) or J147 diet (AD J147) were analyzed by Western blotting and the images quantified in bar graphs accompanying the images.
Whole - cell lysates were prepared from control and infected cells 48 hours after infection.
Cells were harvested at 48 hours following transient transfections with the siRNAs and whole - cell lysates were prepared.
Whole - cell lysates were prepared at 48 hours.
Whole - cell lysates were then prepared at 48 hours.
For the coimmunoprecipitation experiments, HuH - 7 cells transfected with pcDNA - SRα / FLAG - Sp 1 were harvested and cell lysates were prepared using immunoprecipitation lysis buffer [20 mmol / L Tris - Cl (pH 7.5), 150 mmol / L NaCl, 10 % glycerol, and 1 % Triton X-100].
Karpas 299 and SU - DHL1 cells were transiently transfected with 20 μg mTOR siRNA and whole - cell lysates were prepared at 48 hours.
Whole - cell lysates were prepared 24 and 48 hours following treatment.
(E) Immunoblots were performed on whole - cell lysates (pSTAT5, STAT5, and tubulin) or conditioned medium (MMP1, IL - 6, and TNC) of parental 1833 and SCP28 cells.
(F) Immunoblotting with the indicated antibodies were performed on whole - cell lysates (pSTAT5, STAT5, and tubulin) or conditioned medium (MMP1, IL - 6, and TNC) of 1833 cells.
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