Sentences with phrase «cell lysis»
"Cell lysis" refers to the process in which a cell's outer membrane is broken open or disrupted, resulting in the release of its contents. Full definition
Reactions were terminated with 1 ml of ice - cold PBS, and cells were centrifuged and resuspended in 50 μl of cell lysis buffer containing 1 % NP - 40 and protease inhibitors.
immunohorizons.org
Disposable microfluidic cartridges coencapsulate single cells and barcodes into subnanoliter droplets, where cell lysis and barcoding occur.
Cell pellets were resuspended in 1 ml cell lysis buffer [5 mM Pipes pH 8.0, 85 mM KCl, 0.5 % NP - 40] containing protease inhibitors and incubated for 10 min on ice.
The cells were incubated for 15 — 30 minutes at room temperature to allow complete cell lysis before determining luminescent levels using in an Envision plate reader.
Vince guided them through cell lysis (soap), precipitation (salt), and then separation (ethanol).
K563 cell lysis in the treated hu - PBMC co-cultures was observed, also.
The distinction between apoptosis and cell lysis is important in immunology - lysing a virus - infected cell would only release the virions, whereas apoptosis leads to destruction of the virus inside.
The pellet was resuspended in 10 ml of resuspension buffer (20 mM NaHPO4, 300 mM NaCl, 20 mM Imidazole, pH 7.8), and cell lysis was induced by freezing at − 80 °C with PMSF, Leupeptin and Pepstatin added to inhibit protease activity.