Not exact matches
Fluorescent transgenic ES
cell lines - PCR
assays are provided for the
marker transgene.
Methods: Human corneas with attached scleral rims were obtained from eye banks and were
assayed for telomerase activity and BrdU (bromodeoxyridine) incorporation to determine, respectively, the presence of a stem - like
cell marker and replicative activity.
Oligonucleotide - labeled antibodies allow integration of cellular protein and transcriptome measurements at a single -
cell level, with the number of simultaneously
assayed protein
markers far surpassing what can be measured by cytometry - based approaches.
By means of a non-obese diabetic / severe combined immunodeficiency disease (NOD / SCID) xenotransplant
assay in combination with specific
cell surface
markers (CD44 + CD24 - / low), CSCs were enriched from metastatic and primary breast tumors and were shown to have the ability to reestablish tumor heterogeneity after transplantation [1].
It will be important to include
assays of NK
cell markers and functions in future longitudinal studies in order to investigate this point in detail as well as to consider the trace element zinc as an essential co-factor for optimal NK
cell activity.
This
assay detects
cells that are able to give rise to new colonies containing a majority of
cells that express stem
cell markers.
Fluorescence - based
assays Fluorescent
markers such as EGFP, YFP, mCherry and mTomato or fluorescent biosensors can be used to measure a variety of real - time
cell - based activities, including, intracellular transport, protein signaling, receptor desensitization, migration, division, apoptosis, metabolism, differentiation, chemotaxis, transcription and translation.