Sentences with phrase «cell markers oct4»

Large differences in staining for these cell markers were not observed in heterochronic young mice compared with isochronic young mice.

In support, PC3 cell holoclones, but not meroclones and paraclones, express three well - established stem and progenitor cell markers.

We use two stem cell markers to improve the resolution of the analysis, which sorted the population into GCTM - 2HIGHCD9HIGH, GCTM - 2MIDCD9MID, GCTM - 2LOWCD9LOW, and GCTM - 2 − CD9 − (HIGH, MID, LOW, and NEG).

Overall, 15 % of cells co-expressed lineage specific and stem cell markers.

We compared the expression of RPE cell markers in transplanted cells in vivo against cells re-plated and cultured in parallel.

Holoclones express high levels of stem and progenitor cell markers CD44, α2β1 integrin, and β - catenin.

(D) Expression of the same RPE cell markers is maintained in vivo by iPS - RPE (white arrows) 8 days following transplantation into the subretinal space of the RCS rat.

Results: Fibroblast growth factor 2 (FGF2) treatment reduced expression of β - cell markers, (INS, MAFB, SLC2A2, SLC30A8, and GCK) and activated ectopic expression of MYC, HES1, SOX9, and NEUROG3.

(E) Western blot analysis of iPS - RPE protein expression using antibodies against a panel of RPE cell markers.

The GFP+HPC s expressed a whole range of leukocyte and stem cell markers including CD45, CD11b, CD31; relatively low levels of MHC class I; and no class II antigens, as we recently reported [17].

The ΔN isoforms of p63 may also serve as stem cell markers in a variety of epithelial cell types, including endometrium [30], cervix [31], breast [32], prostate [33], and in stratified squamous epithelium [21,34].

Phenotypic characterizations reveal high levels of CD44, α2β1 integrin, and β - catenin expression in holoclones, whereas meroclones and paraclones show markedly reduced expression of these stem cell markers.

This assay detects cells that are able to give rise to new colonies containing a majority of cells that express stem cell markers.

A combination of several germ cell markers will thus be necessary to detect germ cell differentiation from ES cells.

Venus positive cells experiencing low - level transcription at the Hex locus, but still expressing the ES cell markers SSEA - 1 and Oct4, show elevated levels of PrEn gene expression and reduced levels of early ICM markers such as Nanog.

VASA is a valuable gene for the detection of germ cells differentiated from ES cells in monkeys, and the identification and characterization of germ cells derived from ES cells are possible by using reported germ cell markers in vivo, including SSEA1, OCT - 4, and VASA, in vitro as well as in vivo.

Among the early germ cell markers examined, VASA is a candidate gene for detecting pre-meiotic germ cell differentiation from monkey ES cells, because its expression is detected earlier in the primordial stage of germ cell development in comparison to that of PIWI family genes in vivo in mice and humans [11], [36]--[38], [49].

With regard to the meiotic germ cell markers, SCP1 and SCP3 expression was also detected in the testes, whereas MLH1 was expressed in various tissues and ES cells.

In addition, the expression of other germ cell markers, such as NANOS and PIWIL1 genes, was also up - regulated as the EB differentiation progressed.

It will be important to include assays of NK cell markers and functions in future longitudinal studies in order to investigate this point in detail as well as to consider the trace element zinc as an essential co-factor for optimal NK cell activity.

With this reporter we split ES cell cultures into two fractions that both express certain stem cell markers but only one of which expresses low levels of an endodermal marker gene.

Using this cell line we show that apparently undifferentiated ES cell cultures consist of at least three cell types defined by this lineage - specific low - level transcription and the expression of the ES cell markers Oct4 and Nanog.

«At first we blamed ourselves, but then we looked at the cell markers and saw that the cells were aging much faster» than true embryonic cells.

This catalog offers a detailed choice of the most commonly used cell markers and an extensive selection of antibodies used in immunology.

For the detection of specific antigens, the following primary antibodies were used: mouse monoclonal anti-Tuj1 (Covance, Princeton, NJ, USA) as a neuronal marker, rabbit anti-L1 (kindly provided from Dr. Asou)[52] and rat monoclonal anti-L1 (Millipore, Billerica, MA, USA) as granule cell markers, rabbit anti-calbindin D28K (Millipore) as a Purkinje cell marker, and rabbit anti-glial fibrillary acidic protein (GFAP, DAKO, Denmark) as an astrocyte marker.

Title: Single - molecule transcript counting of stem - cell markers in the mouse intestine Authors: Itzkovitz S, Lyubimova A, Blat IC, Maynard M, van Es J, Lees J, Jacks T, Clevers H, van Oudenaarden A Date: 2011 Publication Details: Nat Cell Biol.

Cell markers are helpful tools used to identify a specific immune cell population.

Significance of CD44 and CD24 as Cancer Stem Cell Markers: An Enduring Ambiguity.

Solid tumours are reported to possess a sub set of cells called Side Population expressing various stem like cell markers, self - renewal capacity and ability to give rise to differentiated cells [23, 24].

Corresponding to upregulated TRF2 expression at 80μM, stem cell markers like CD44 and Oct4 were also observed to be upregulated in Clonospheric HCT116 as compared to Parental HCT116 (Fig. 3a and 3b) Decreasing CD24 expression in clonospheric HCT116 was observed which correlated well with increased stem cell expression (Fig. 3b).

Human T - cell leukemia virus type I Tax induces the expression of dendritic cell markers associated with maturation and activation.

There are no specific markers to identify them; however, they are negative for hematopoietic cell markers like CD34 / 45 / HLA - DR and express CD90 / 73/105 on their surface 2, 3, 4.

Further research uncovered a broad spectrum of cell surface stem cell markers (e.g., CD133, CD44, and CD24) that allow the identification of CSCs in human solid tumors, including brain, breast, prostate, pancreas, liver, ovary, skin, colon cancers, and melanoma (3 - 6)(Figure 1 based on 7).

Cancer stem cell markers are enriched in normal tissue adjacent to triple negative breast cancer and inversely correlated with DNA repair deficiency.

Perivascular cells, including pericytes in the smallest blood vessels (e.g., microvessels) and ARCs around larger ones, express mesenchymal stem cell markers and bear a multi-differentiation fate potential (differentiate into osteoblasts, chondrocytes, adipocytes, smooth muscle cells and myocytes) similar to that documented for MSCs in vitro.

To verify that the individual cells they picked were pure, the researchers cultured them for several days to determine, using cell markers, whether they formed mature HSCs.

The ECETOC Young Scientist Award at the European Societies of Toxicology (Eurotox) Annual Congress (Rhodes, Greece, 5 - 8 October 2008) went to: Ms Nathalie Lambrecht of CARDAM VITO in Belgium for her poster, entitled «Pathway analysis of dendritic cell markers for skin sensitization.»

At one end of the neuroepithelium, a region developed that was positive for markers of progenitors of granule and deep cerebellar nuclei projection neurons and negative for Purkinje - cell markers, and whose origins could be traced to the rhombic lip zone of the cerebellar plate.

They found that cells treated with FGF2 expressed late Purkinje - cell markers and developed structures characteristic of those cells.

«You have blood drawn and that one blood sample can be tested for a comprehensive array of cancer cell markers.

A stem cell is generally characterized by the presence of various proteins — or cell markers — on its outer surface.

They found that ONC201 alters the gene expression of cancer stem cell markers and signaling pathways prior to killing the tumor cells, providing pharmacodynamic biomarkers of response.

«Eighty - two percent of the smooth muscle cells within advanced atherosclerotic lesions can not be identified using the typical methodology since the lesion cells down - regulate smooth muscle cell markers.

One common application for quantum dots is to use them as cell markers in biomedical research, says Todd Krauss, an associate professor of chemistry and optics at the University of Rochester's Institute of Optics who studies the fundamental properties of quantum dots as well as their optical properties.

The figure in question is supposed to show patterns of expression for a range of ES cell markers in the 11 cell lines.

In 1988 and 1989 at Edouard Herriot Hospital, two fetuses whose blood cell markers showed they had severe immune disorders became the first ever to receive a cell transplant.

The infected cells were multinucleated syncytia and expressed the S100 and p55 dendritic cell markers.

Cells grown on hydrogels of the same stiffness all display fat cell markers and deform the underlying matrix material the same way.

«We couldn't have figured out what was happening without the expertise of our collaborators at U.C. Davis, who determined the genetic sequence of every single cell marker on the cells in the lymph nodes,» says Miller.

«We can direct them to CD45, a cell marker which is exclusively expressed in the blood system.