Sentences with phrase «cell mass spectrometry»
Her experience includes investigating the neuroinflammatory response in Multiple Sclerosis and several neurodegenerative diseases, using single - cell mass spectrometry approaches.
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She was always ready to learn new techniques, and she diversified her skill set by working in the animal facility, cell culture room, and also in a mass spectrometry lab.
«During the development of specialized tissues from stem cells, enzymes belonging to the Tet family also oxidize the thymidine base, as we have now shown with the aid of highly sensitive analytical methods based on mass spectrometry.
The research team used mass spectrometry to compare phosphorylation of proteins from mouse embryonic stem cells with fully functioning GSK - 3 to cells in which the gene encoding GSK - 3 had been deleted.
They used an analytical technique called quantitative mass spectrometry to identify mitochondrial inner membrane proteins which are reduced in cells without OXA.
A strategic focus is to continue to develop computational tools (such as KinomeXplorer, NetworKIN, and NetPhorest) and to deploy these on genome - scale quantitative data obtained by, for example, mass spectrometry, genomic, and phenotypic screens to understand the principles of how spatio and temporal assembly of mammalian signaling networks transmit and process information at a systems level in order to alter cell behavior.
Using quantitative mass spectrometry to search for proteins that contained these stable isotope labels, researchers were able to determine the cell of origin of both intracellular and secreted proteins identified in multicellular culture.
Conventional technology such as mass spectrometry and nuclear magnetic resonance can measure such components — that is how biochemists test the toxicity of drugs or environmental pollutants on human cells.
The researchers used a multitude of screening tools in this study to analyze the molecular components in the blood, including mass spectrometry - based quantitative proteomic, metabolomic and hematological analyses of blood cells.
To identify the main compounds (metabolites) produced by glioblastoma cells during infection by Zika, the researchers analyzed the cells using matrix - assisted laser desorption / ionization mass spectrometry imaging (MALDI - MSI).
In the case of the Circulation paper, which reported a surprisingly high turnover rate for muscle cells in the adult heart, Leri and Anversa say that Kajstura apparently altered, without their knowledge, data from mass spectrometry experiments performed at Lawrence Livermore National Laboratory (LLNL) in California.
Ding said recent advances in a technology called mass spectrometry and in techniques to analyze massive quantities of data have made complex studies of the proteins in tumor cells possible.
Dr. Veenstra joined NCI - Frederick in the fall of 2001 to implement a state - of - the - art mass spectrometry center that focuses on the discovery of signaling pathways and networks that are activated within cells as a response to external perturbations.
Two - dimensional polyacrylamide gel electrophoresis combined with chromatographic phosphoprotein pre-enrichment and electrospray ionization mass spectrometry - based protein identification was used to reveal changes of phosphoproteins in cells exposed to cyclic stretching.
The main tool they used is called CyTOF, which relies on mass spectrometry to measure many parameters on individual cells.
In the present study, the researchers have developed a mass spectrometry - based method that is sensitive and reproducible in order to measure, at steady - state conditions, absolute protein copy numbers across human tissues and cell lines and compared these levels with the corresponding mRNA levels using transcriptomics.
Mats Nilsson demonstrated a unique technology to reveal cells harboring specific mutations in tissue sections, while Ruedi Aebersold held an impressive lecture, illustrating about how powerful mass spectrometry provides a basis for for computational analyses, commented moderator Ulf Landegren.
Pulling FP - binding proteins out of the entire proteome of test cells and identifying them using mass spectrometry techniques, the team matched nearly all to known hydrolases.
Other areas of instrument development include DNA sequencing, cell fractionation, light and electron microscopy methods, mass spectrometry of proteins, X-ray imaging plates, synchrotron beam - lines and automated cell micro injectors.
At the heart of mass spectrometry is the ability to precisely measure tiny samples of substances such as chemicals in soil and water or cancer cells in a tissue sample.
The core will provide assistance with project design, testing of BioID - fusion proteins, establishment and testing of cells expressing BioID - fusion proteins, large scale BioID pull - downs, arranging for candidate identification by mass spectrometry and data analysis.
Decreased costs, increased analysis speed and easier access to mass spectrometry instruments and user - friendly software will have a major impact in cell biology research.
We discovered several Eph receptors and ephrins, and research in our laboratory is dedicated to the characterization of Eph receptor signal transduction mechanisms and biological functions using biochemical, mass spectrometry, molecular biology and cell biology approaches in conjunction with animal models.
Quantitative mass spectrometry and gene expression arrays were used to investigate the consequences of BAP1 expression modulation in the NCI - H226 mesothelioma cell line.
Here we characterise Myst2 / Kat7 / Hbo1 protein interactions in mouse embryonic stem cells by affinity purification coupled to mass spectrometry.
Cells were then washed in medium, and metabolites were extracted with acetone and analyzed by reverse - phase high - performance liquid chromatography (HPLC) and by electrospray mass spectrometry as described previously (19).
His career success began in 2006 at Celgene Corporation, where he was the principal investigator behind a highly interdisciplinary program focused on mass spectrometry - based proteomic studies of placenta - derived populations of stem cells and natural killer cells.
Laboratory: Experience with laboratory applications in parasitology, immunology and molecular biology including, microscopy, BCA Protein Assay, ELISA, Flow Cytometry, Real Time qPCR, Luminex, Gas chromatography (GC) mass spectrometry, cotinine assays (saliva), magnetic bead cell separation (MACS, Percoll and Ficoll) and whole blood cultures.
Proteomes covers the multi-disciplinary topics of structural and functional biology, protein chemistry, cell biology, methodology used for protein analysis, including mass spectrometry, protein arrays, bioinformatics, HTS assays, etc..