Sentences with phrase «cell microarray»

She's also created artificial bone materials, targets for tuberculosis therapy, cell microarray platforms, and a cell nanoinjector, a device used to inject molecules into living cells.

We can make cell microarrays that contain a very large percentage of the human genome without being limited by the protein's properties,» says Sabatini.

Not exact matches

Eager to sort through the pile of curriculum vitae's on his desk, Rudnicki is particularly looking for molecular geneticists working on different stem cell systems; scientists with genomics bents like proteomics, microarray analysis, and bioinformatics; and a couple of clinician scientists.

The scientists then scanned the samples using microarray technology, which cuts genetic material into segments to provide a snapshot of which genes are active and which are asleep inside the cells.

This study, published in the journal Microarrays, shows that lack of SOST in the bone microenvironment promotes the expression of many genes associated with cell migration and / or invasion, including long non-coding RNA MALAT1 in prostate cancer, suggesting that SOST has an inhibitory effect on prostate cancer invasion.

ONE OF THE FIRST THINGS MIKOVITS DID was to employ a microarray — a small tray seeded with DNA from nearly every known virus — to flag viral DNA in human white blood cells.

And he had some preliminary evidence: When he added DNA from malignant prostate tissue cells to a microarray, a novel retrovirus emerged.

The Ogretmen laboratory screened previously reported microarray data sets of several human tumor tissues (metastatic head and neck squamous cell carcinoma, melanoma, and renal cell carcinoma) and showed that, in these samples, only the levels of CerS4 were significantly decreased.

Microbial pathogens induce specific host responses or «RNA biosignatures» that can be identified using microarray analyses of blood leukocytes (white cells).

Subjecting N. gonorrhoeae to the phenotypic microarray screening method for the first time, Sikora's team focused on seven proteins from the bacteria's cell envelope, which consists of the outer membrane, the cell wall and the inner membrane.

The goal of the first experiment was to see whether lncRNAs are differentially expressed in prostate cancer by measuring total RNA from prostate cancer cell lines and normal epithelial prostatic cells using NCode human ncRNA array and SurePrint G3 human lncRNA microarrays.

Melton and colleagues are now doing large scale screening with microarrays to track gene activity as cells develop and discover gene products that move cells along the proper paths.

Using microarrays that enabled them to examine 100,000 sites on the genome, they looked at the cells» nuclear DNA, mitochondrial DNA, and methylation patterns (which give clues to whether a gene has been turned on or off).

Periostin (POSTN) was identified by microarray RNA profiling studies as a stromal factor involved in maintaining the normal stem cell niche and demonstrated to be secreted by stromal fibroblasts, but not by infiltrating tumor cells (Malanchi et al., 2012).

The Monash BDI researchers worked in collaboration with Canadian - based company Kinexus, and used an antibody microarray to simultaneously investigate hundreds of factors involved in cell signalling that were modulated by HCV replication, including human protein kinases.

First author Dr Reza Haqshenas said the researchers then used gene silencing technology to determine whether the genes» cell factors identified using the antibody microarray were indeed important for HCV replication and therefore potential targets for anti-HCV compounds.

Microarray data sets for pluripotent cells (PC), EFTF - PC, EF, PNP, LE, and whole embryos (WE) are shown.

Epifluorescence image of HeLa S3 cells grown on top of a two - molecule microarray.

We are now expanding this facility to not only include state - of - the - art protein domain microarrays, but also to allow the further characterization of these protein - protein interactions in cells.

The analysis of doxorubicin resistance in human breast cancer cells using antibody microarrays.

Therefore, we used microarray analysis to perform pairwise comparisons of the transcriptional profile of EFTF - expressing pluripotent cells to three regions of the Xenopus embryo: the eye field (EF) including underlying mesendoderm, posterior neural plate (PNP) and the non-neural, flank, lateral endoderm (LE, Figure 1A).

The department has expertise and infrastructure to support bacterial and viral analysis, and the detailed evaluation of cellular and serological immune responses including flow cytometry and cell sorting, Luminex multiplex platforms, real time PCR, microarrays, comprehensive histological staining, immunohistochemistry and in - situ hybridisation.

The facility collaborates with various research projects using flow cytometric techniques to isolate and purify cells and chromosomes for structural and functional studies, sequencing, microarrays as well as fluorescence insitu hybridisation (FISH) experiment.

Protein subcellular localization profiling of breast cancer cells by dissociable antibody microarray staining.

To assess the expression of Tie2 and other Ang / Tie signalling molecules on human pericytes, a microarray - based expression profiling of human brain pericytes (BP), placenta pericytes, pancreas pericytes (PA), lung pericytes (LP), muscle pericytes (MP) and human umbilical vein endothelial cells (HUVEC) was performed.

Patients and Methods: A tissue microarray (TMA) consisting of four biopsy cores from 34 matched pairs of nephrectomy and metastatic sites of clear cell RCC was used to assess PD - L1 expression by quantitative immunofluorescence.

Study of stem cell function using microarray experiments.

He'd like a way to perform single - cell analysis using microarrays, instead of full sequencing, but chip - based bisulfite methods require more nucleic acid — about a microgram — than one cell can provide.

DS carried out cell culture experiments and JLE performed DNA microarray assay and corresponding data analysis.

Paired SAGE - microarray expression data sets reveal antisense transcripts differentially expressed in embryonic stem cell differentiation.

Other proteins can't be put on a microarray at all because they are part of a cell membrane and need to be attached to their membranes to function.

Microarray profiling identified STAT6 as one of the proteins whose expression was increased in apoptosis - resistant cells (Gagarin et al., 2005).

For some applications, a qPCR wil... Keywords: Amplification, Cancer, Capillary electrophoresis, Cell culture, Degradations, Detection methods, Electrophoresis, Gene expression, Growth factors, Indicators, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA purification, Sample preparations, Transcription

Similarly, microarray analyses comparing global gene expression in cells that overexpress wild - type STAT3 or phosphorylation - deficient mutant indicate that a subset of genes are regulated by unphosphorylated STAT3, including well - known oncogenes such as MRAS and MET (Yang et al., 2005).

Researchers performed ES cell cultures, RNA preparations and validated microarray data: IA KA NB PYB MXD MG EK SL MR AR MPS MT. Results were discussed among all Consortium partners.

However, normalization is typically performed using methods developed for bulk RNA sequencing or even microarray data, and the suitability of these methods for single - cell transcriptomics has not been assessed.

Microarray profiling using the Affymetrix Mouse Genome 430 2.0 array, defines sets of genes that are specific to bone marrow - derived hematopoietic stem cells and their different immunological progeny.

Breeden, Prentice and Zhao, whose study appeared in the May 8 Proceedings of the National Academy of Sciences, compared their new algorithm to other methods used to analyze microarray experiments designed to identify cell cycle genes in yeast.

The microarrays reproducibly delineated previously characterized gains and deletions in a cancer cell line and identified a small gain not detected by use of conventional CGH.

Specifically, we have generated clusters of transcripts that behave the same way under the entire spectrum of the sixty - seven experimental conditions; we have assembled genes in groups according to their time of expression during successive days of ES cell differentiation; we have included expression profiles of specific gene classes such as transcription regulatory factors and Expressed Sequence Tags; transcripts have been arranged in «Expression Waves» and juxtaposed to genes with opposite or complementary expression patterns; we have designed search engines to display the expression profile of any transcript during ES cell differentiation; gene expression data have been organized in animated graphs of KEGG signaling and metabolic pathways; and finally, we have incorporated advanced functional annotations for individual genes or gene clusters of interest and links to microarray and genomic resources.

This is accomplished by combining high - throughput generation of affinity - purified antibodies with protein profiling in a multitude of tissues and cells assembled in tissue microarrays.

Microarray studies have the potential to piece together groups of co-regulated genes and thus lead to the discovery of novel components of genetic pathways in ES cells.

Recently, a large - scale microarray study characterized gene expression differences between current, former, and never smokers [5], and identified specific genes related to xenobiotic functions, anti-oxidation, cell adhesion and electron transport to be more highly expressed in current smokers relative to never smokers.

Tags for this Online Resume: next generation sequencing, microarray analysis, bioconductor, perl, bioperl, bioinformatics, c, MySQL, microbiology, BSL2, BSL3, DNA extraction, ELISA, cell culture, laboratory

Q - MeDIP analysis of DNA methylation differences between CPA (black) and control (white) groups in men and women T cells samples for four gene promoters predicted to be more methylated in the men CPA group by microarray analysis.