Sentences with phrase «cell microscopy technique»

The team has succeeded not only in deciphering what is happening in the cell interior but also, using a revolutionary live - cell microscopy technique, the scientists have observed for the first time individual receptors at work in intact cells.

Not exact matches

The latest in live - cell microscopy — multiphoton imaging, light - sheet techniques, and technology borrowed from Raman spectroscopy — allow researchers to study living cells in more detail with less effort.

«At the very microscopic level,» he says, «we have developed techniques like two - photon microscopy, which allows extremely detailed examinations of structures and processes within cells.»

Dr. Michael Man, a postdoctoral fellow in Prof. Dani's Unit, combined the techniques of UV light pulses and electron microscopy in order to see electrons moving inside a solar cell.

The technique could also be modified for microscopy, allowing imaging of photosynthesis inside the plant cells.

The technique, called stimulated Raman scattering (SRS) microscopy, generates videos of moving cells deep inside tissue and could replace biopsies in the diagnosis of cancers.

The technique, called superresolution single - molecule fluorescence microscopy, recently helped scientists at the University of Manchester in England track natural killer (NK) cells, which help destroy cancer and viruses.

In the lab, the team mixed each strain of K. veneficum with a species of algae on which it preys, and recorded the three - dimensional motions of thousands of cells using a high - speed holographic microscopy technique they described in 20071.

Using an advanced imaging technique called intravital microscopy, the researchers followed the movement and interaction of HIV - infected cells in the spleen of mice.

Backman has been studying cell abnormalities at the nanoscale in many different types of cancers, using an optical technique he pioneered called partial wave spectroscopic (PWS) microscopy.

Besides devising unique ways to analyze conventional cell images, researchers are also using new microscopy techniques to better define cell shapes.

This allowed the researchers to blow random puffs of air at their faces, causing them to blink, and to use a non-invasive microscopy technique to look at how the relevant Purkinje cells respond.

The research involved Professor Frederic Meunier's laboratory at QBI, where super-resolution microscopy techniques enabled the researchers to understand how the anaesthetic worked on single cells.

«We studied a range of authentic microfossils using the same transmission electron microscopy technique and in all cases these reveal coherent, rounded envelopes of carbon having dimensions consistent with their origin from cell walls and sheaths.

In this role, she works with many collaborators to facilitate implementing superresolution microscopy into their research programs as well as developing novel techniques for microbial live cell imaging.

learn from our speakers the benefits of imaging live cells using techniques such as high resolution microscopy, superresolution microscopy, and high - content analysis

The breakthrough came with a new imaging technique, dual - resonance - frequency - enhanced electrostatic force microscopy (DREEM), which was developed by University of North Carolina at Chapel Hill chemist and co-author Dorothy Erie, former UNC and NC State postdoctoral researchers Dong Wu and Parminder Kaur, and was featured earlier this year in Molecular Cell.

To find answers, Columbia researchers developed a new microscopy technique that allows for the direct tracking of fatty acids after they've been absorbed into living cells.

By making the switch, all molecules made from fatty acids can be observed inside living cells by an advanced imaging technique called stimulated Raman scattering (SRS) microscopy.

Another popular technique for 3D cell imaging called spinning disc confocal microscopy can't image samples nearly as long as Betzig's technique, but it is still better for imaging thick cells and tissue.

The microscopy techniques that permit imaging of brain cells in awake mice generally can't visualize anything deeper than a fraction of a millimeter below the brain's surface, whereas the mPOA is several millimeters deep.

Super-resolution microscopy is a technique that can «see» beyond the diffraction of light, providing unprecedented views of cells and their interior structures and organelles.

A common technique is fluorescence microscopy, where fluorescent molecules («probes») are attached to cell structures and then «lit up» against a dark background.

Current microscopy techniques can resolve details as small as 2 nanometres in prepared samples and 200 nanometres in living cells.

The researchers used electron microscopy and other imaging techniques to view thousands of cells from the liver tissue of lean and obese mice.

Powerful new microscopy techniques enable researchers to observe the whole process in living cells, with bright fluorescent tags highlighting the chromosomes and other cellular components.

In the current study, the researchers used high - affinity antibodies to «label» the cannabinoid receptors so they could be seen using various microscopy techniques, including electron microscopy, which allowed very detailed visualization at individual synapses, or gaps between nerve cells.

Unlike atomic force microscopy, microfluidics is a high - throughput screening technique, but additional work is required to assess the efficiency of this type of biophysical - based sorting for stem cell enrichment.

Use of the immunofluorescent microscopy visualization technique reveals the presence of specific proteins in naive human embryonic stem cells.

By developing a new fluorescence microscopy - based technique, the researchers were able to measure how long it takes proteins to move over distances ranging from 0.2 to 3 micrometres in living cells.

New techniques combining various staining methods with electron and light microscopy make it possible to investigate in detail the connections among nerve cells and the circuitry of the brain

A new technique enables 3 - D visualization of chromatin (DNA plus associated proteins) structure and organization within a cell nucleus (purple, bottom left) by painting the chromatin with a metal cast and imaging it with electron microscopy (EM).

By parsing the cells with slices of laser light and then correcting for any obstruction with the same AO technique astronomers use to correct blurriness in observations of stars, the scientists have come up with a microscopy technique that looks like an artistic rendering.

She has extensive research experience in the development and application of novel electron microscopy techniques for energy materials, such as lithium ion battery materials and fuel cell catalysts.

Colocalization analysis in fluorescence microscopy Cell Imaging Techniques (pp. 97 - 109): Springer.

As they develop microscopy techniques to better visualise the details of chromatin structure, even in living cells, they're better able to explore how structural changes relate to gene expression and cell function.

We work across disciplines and use a variety of techniques including microfluidics, standard microscopies (electron, optical, fluorescence, confocal), spectroscopies (fluorescence, UV, CD), scattering techniques (X-ray, light), protein expression and characterization and cell - free gene expression to investigate the utility of coacervate microdroplets as robust reaction compartments and cellular mimics.

Furthermore, the microscope will be capable of performing live - cell super-resolution imaging through structured illumination microscopy (SIM) and Super-Resolution Radial Fluctuations (SRRF); for fixed cells resolutions on the scale of tens of nanometres will be achievable using single molecule localization microscopy (SMLM) techniques.

In the 1940s, Porter was one of the first in the world to use the revolutionary technique of electron microscopy (EM) to reveal the internal structure of cells.

His team applied, among others, techniques of confocal microscopy and cell sorting by flow cytometry which led to the discovery in human muscle biopsies that these myoendothelial cells are located adjacent to the walls of blood vessels.

They also assist with more advanced techniques, such as live - cell microscopy, three - dimensional reconstruction, and image analysis.

The Kind group studies the regulation of gene expression in single cells by developing and using novel microscopy and genomics based techniques.

We develop methods to label RNA in fixed and living cells using fluorescent probes and microscopy techniques and image analysis algorithms to visualize and quantify many mRNAs simultaneously.

Recent progress in phylogenomics, and the implementation of modern molecular, microscopy, and cell biology techniques in a handful of spiralian model systems have made that possible.

To uncover molecular processes in individual cells and to understand the full complexity of biological systems, our lab applies and develops novel microscopy and genomics based techniques to study the regulation of gene - expression in single cells.

The projects are multidisciplinary, involving a variety of techniques including molecular biology (PCR, cloning), cell culture and advanced fluorescence microscopy.

The team has systematically validated the fidelity, accuracy, reliability, reproducibility and versatility (for many cell types) of this method, using various other techniques such as confocal microscopy, flow cytometry, Western blotting and Raman spectroscopy.

Other structural biology techniques, such as cryo - electron microscopy, can not resolve cellular elements beyond an intact cell's surface, explains Julia Mahamid of the Max Planck Institute for Biochemistry, a member of the research team (Science 2016, DOI: 10.1126 / science.aad8857).

In cases of neonatal mortality, the diagnosis typically is made postmortem with virus isolation from fresh lung, liver, kidney, and spleen by cell culture techniques and subsequent identification by PCR and sequencing, transmission electron microscopy, immunofluorescence, or fluorescence in situ hybridization.

Professional Duties & Responsibilities Biomedical and biotechnology engineer with background in design of biomaterials, biosensors, drug delivery devices, microfrabrication, and tissue engineering Working knowledge of direct cell writing and rapid prototyping Experience fabricating nanocomposite hydrogel scaffolds Proficient in material analysis, mechanical, biochemical, and morphological testing of synthetic and biological materials Extensive experience in bio-imaging processes and procedures Specialized in mammalian, microbial, and viral cell culture Working knowledge of lab techniques and instruments including electrophoresis, chromatography, microscopy, spectroscopy, PCR, Flow cytometery, protein assay, DNA isolation techniques, polymer synthesis and characterization, and synthetic fiber production Developed strong knowledge of FDA, GLP, GMP, GCP, and GDP regulatory requirements Created biocompatible photocurable hydrogels for cell immobilization Formulated cell friendly prepolymer formulation Performed surface modification of nano - particle fillers to enhance their biocompatibility Evaluated cell and biomaterial interaction, cell growth, and proliferation Designed bench - top experiments and protocols to simulate in vivo situations Designed hydrogel based microfluidic prototypes for cell entrapment and cell culture utilizing computer - aided robotic dispenser Determined various mechanical, morphological, and transport properties of photocured hydrogels using Instron, FTIR, EDX, X-ray diffraction, DSC, TGA, and DMA Assessed biocompatibility of hydrogels and physiology of entrapped cells Evaluated intracellular and extracellular reactions of entrapped cells on spatial and temporal scales using optical, confocal, fluorescence, atomic force, and scanning electron microscopies Designed various biochemical assays Developed thermosensitive PET membranes for transdermal drug delivery application using Gamma radiation induced graft co-polymerization of N - isopropyl acylamide and Acrylic acid Characterized grafted co-polymer using various polymer characterization techniques Manipulated lower critical solution temperature of grafted thermosensitive co-polymer Loaded antibiotic on grafted co-polymer and determined drug release profile with temperature Determined biomechanical and biochemical properties of biological gels isolated from marine organisms Analyzed morphological and mechanical properties of metal coated yarns using SEM and Instron Performed analytical work on pharmaceutical formulations using gas and high performance liquid chromatography Performed market research and analysis for medical textile company Developed and implement comprehensive marketing and sales campaign