Sentences with phrase «cell nucleic acid»

The sample was injected with live cell nucleic acid stain [STYO 13] which is taken up by live bacteria and shows as bright green when viewed using a 500 nm - long pass emission filter for fluorescence detection.

These improvements push the boundaries of nucleic - acid detection and fragment sizing, affecting applications such as single - cell nucleic acid analysis, PCR - free preparation of NGS library preparation, bacterial artificial chromosome clone sizing, and exosome analysis.

Probes and cell nucleic acids on each slide were co-denatured for 3 min at 73 °C and immediately hybridized for 16 — 24 hours at 37 °C on a ThermoBrite (Abbott Molecular, Inc.).

Not exact matches

For example, after the macromolecules of amino acids or nucleic acids have become sufficiently abundant, the possibility of living cells becomes a relevant new form of ordered novelty in the world's advance.

«It's difficult to make contrived samples behave like actual cell - free nucleic acids, so that's been quite a challenge,» says Kelli Bramlett, director of R&D in clinical sequencing and oncology at Thermo Fisher Scientific in Waltham, Massachusetts.

He studies DNA and RNA quadruplexes, nucleic acid structures that were first visualized via confocal microscopy in fixed, dead cells.

Even after initial purification of the sample, liquid biopsies typically contain much more nucleic acid from healthy cells than from tumors.

My colleagues and I first undertook to synthesize nucleic acids outside the living cell, with the help of cellular enzymes, in 1954.

The field segregates into three major strategies: detecting free nucleic acids, isolating intact circulating tumor cells, and identifying extracellular vesicles from tumors.

The spherical structure of the SNAs is the ideal architecture for delivering nucleic acids into cells for gene regulation, Mirkin said.

The exterior of the nanoparticle is coated with nucleic acids that act as targeting agents, drawing the delivery system to the retina and facilitating uptake by RPE cells.

That is, they depend on the host cell for the raw materials and energy necessary for nucleic acid synthesis, protein synthesis, processing and transport, and all other biochemical activities that allow the virus to multiply and spread.

They naturally enter cells by engaging scavenger receptors that are not recognized by linear nucleic acids.

To start, she used a cotton swab to get a sample of cells from her cheek, boiled them in a test tube in her kitchen to free the DNA, then added primers, nucleic acids that mark the part of the sequence.

The findings, from the Emory / Johns Hopkins / Florida State team that showed this spring that neural progenitor cells are particularly vulnerable to Zika infection, were published in Nucleic Acid Research.

However, in the past decade scientists realized that exosomes play important roles in many biological functions through capsuling and delivering molecular messages in the form of nucleic acids and proteins from the donor cells to affect the functions of nearby or distant cells.

The new chimera interferes with HER2 and HER3 signaling and ultimately leads to cancer cell death, as shown in the group's recent publication in Molecular Therapy: Nucleic Acids.

The first application of the Helinx technology, the Intercept Blood Systems, exploits the fact that while blood components such as red blood cells, plasma, and platelets do not contain nucleic acids, viruses and bacteria do.

An HPV test, in which doctors test a cervicovaginal specimen for the presence of the nucleic acids of carcinogenic types of HPV, is more sensitive than the Pap test (a microscopic examination of exfoliated cells) for detection of precancers.

In a trial described in Nature Materials, the team showed they could deliver the nucleic acids DNA and siRNA into human cells in the lab, using the nanoneedles.

They can penetrate the cell, bypassing its outer membrane, to deliver nucleic acids without harming or killing the cell.

Scientists are currently investigating ways of using nucleic acids to re-program cells to carry out different functions.

They can also deliver precise doses of drugs, nucleic acids, or proteins to targeted points on the cell.

While James Watson and Francis Crick are considered the godfathers of DNA, Hoerr says, Miescher never received the credit he deserved for isolating and analyzing nucleic acids from pus cells.

Cells were stained with the actin binding molecule, phalloidin (red) and nucleic acid stain, DAPI (blue).

For tumors to grow and spread, cancer cells must make larger than normal amounts of nucleic acids and protein, so they can replicate themselves.

In the study published in Nucleic Acids Research, the researchers were able to induce and inhibit the expression of genes in mammalian cell cultures and were able to regulate intracellular protein levels using light signals.

Anderson and Langer have previously developed nanoparticles, now in clinical development, that can deliver siRNA to liver cells called hepatocytes by coating the nucleic acids in fatty materials called lipidoids.

The immune system detects viruses when viral nucleic acids bind protein receptors in immune cells.

But what keeps these receptors from reacting to the host cells» own nucleic acids in the cytoplasm and causing unwanted inflammation?

«We had previously learned that messenger RNA, a nucleic acid present in all living cells whose primary role is to act as a messenger carrying instructions from DNA, moves between parasitic plants and their hosts, but we have yet to discover the significance of this exchange.

The two cell types are important to a broad range of organ systems in the body and play active roles in diseases that could be targets for nucleic acid therapies.

This realm of research is called epigenetics, and at the heart of it is chromatin — the nucleic acids and proteins that package DNA to fit inside cells.

Genetic therapies, such as those made from DNA or RNA, face challenges because of the difficulty in delivering the nucleic acid to the right cells.

Intrigued by the ability of certain polymers to mop up DNA and RNA for gene transfer, Sullenger and colleagues tested the idea that these chemical compounds might also be effective targeting such nucleic acids as they arise in cell death.

Colon cancer cells produce proteins, fatty acids, nucleic acids and reactive oxygen species (ROS).

But it's still a mystery how the amino acid building blocks were first assembled according to coded nucleic acid templates into the proteins that formed the machinery of all cells.

Proteins, fatty acids, and nucleic acids are essential factors for cell survival using glutamine metabolism.

Investigations of how short chains of nucleic acids replicate themselves in vitro have even provided clues to primitive genetic codes for translating nucleic acid information into protein information, systems that could have preceded the elaborate machinery of ribosomes and activating enzymes with which cells now manufacture protein.

He has manufactured intricate molecular rings containing carbon, nitrogen, and hydrogen; fatty - acid - like molecules that look and behave like the membranes protecting living cells; and nucleic acids or nucleotides, the primary components of RNA and DNA.

Another idea is to probe the mother's bloodstream for cells and nucleic acids shed by the placenta as a window into the function of the organ.

Using sensitive polymerase chain reaction (PCR) tests to scour through Brown's blood cells, plasma, and rectal tissue, Yukl and others were able to were able to detect bits of viral nucleic acid.

miRNA - 335, a messenger nucleic acid, regulates the endodermal transcription factors Sox17 and Foxa2 and is essential for the differentiation of cells within this germ layer and their demarcation from the adjacent mesoderm.

However the team from the Krebs Institute for Nucleic Acids at the University of Sheffield found that the key to preventing resistance to a common class of chemotherapy used to treat breast and colon cancer is to change the speed in which the cancer cells repair damage to their DNA that is introduced by chemotherapy.

«We identified a set of novel microRNAs that are potent inducers of neuroblastoma cell differentiation and found that mimics (synthetic fragments of nucleic acid used to raise microRNA levels in cells) of some of the identified microRNAs are much more potent in inducing neuroblastoma cell differentiation than the current differentiation treatments.

Exosomes are tiny packets of proteins, microRNA and nucleic acids that cells emit into their environment to both communicate with neighboring cells and influence their behavior.

He soon managed to restrict the growth factor to a fraction of the tumor cells containing both protein and nucleic acid.

Within a biofilm, cells are typically embedded in a matrix of extracellular polymeric substances (EPS) made of proteins, lipids, nucleic acids and polysaccharides [6 — 8].

This reprogramming methodology dispenses with the standard nucleic acid - mediated strategy and instead treats cells with conditioned media (CM) designed to mimic the primitive embryonic environment.

TNGB collects, stores and distributes high quality biological materials (nucleic acids, cell lines and tissue samples) linked to relevant clinical and genetic data from patients affected by inherited diseases.