Sentences with phrase «cell pellet»
A "cell pellet" refers to the solid clumps of cells that are gathered together at the bottom of a tube or container after the liquid portion (supernatant) is removed. It's like a group of cells that have settled down, similar to how sand settles at the bottom of a bottle. Full definition
Crude genomic DNA extracts (gDNA) of 78 AID - negative Amrose subclones were prepared by incubation of cell pellets of app.
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Ficoll purified peripheral blood mononuclear cell pellets of the sort used in the current investigation are comprised of several different cell types (e.g., CD4, CD8, CD14, CD19, CD56)(Reinius et al., 2012).
Both TMA blocks contained identical cell lines, cored from cell pellets, as previously detailed for normalization between TMA blocks.
The laboratory also offers frozen sectioning, special staining, processing of cell pellets, alcohol fixation, DNA / RNA and LCM preparation and sectioning, and consultations on research protocols.
Mononuclear cell pellets were separated from the diluted blood specimen by centrifugation with ficoll (400 g, 30 min) and the mononuclear cell layer was removed from the tube using a transfer pipette, resuspended in a phosphate buffered saline solution, and briefly centrifuged again.
Perform protein extraction from tumor tissue or cell pellets, upon request (with additional charge), for use in the reverse phase protein array studies (see pricing below).
Collected cells were centrifuged at 4 °C for 5 min and the cell pellets were treated with ice - cold cell extraction buffer containing protease and phosphatase inhibitor for 30 min, on ice, with vortexing at 10 min intervals.
Cell pellets were then resuspended in 40 % Percoll.
Cell pellets were resuspended in 1 ml cell lysis buffer [5 mM Pipes pH 8.0, 85 mM KCl, 0.5 % NP - 40] containing protease inhibitors and incubated for 10 min on ice.
Cells were centrifuged and the cell pellet was resuspended.
Cell pellets were washed with PBS.
Cell pellets were resuspended in phosphate - buffered saline, total cell count was performed, and then cytospins were prepared with 5000 cells.
The cell pellet was resuspended in 12 µl of the manufacturers resuspension buffer per transfection / plasmid (Invitrogen); and 0.5 µg of GFP plasmid; 1 µg of Smad4 siRNA vector [17]; 100 nM of control, Smad1 or Smad5 siRNA (Life Technologies); or 100 nM of control miRNA or miR - 181a inhibitor / antagomir (Life Technologies) was added to the cell suspension.
Cells were washed 3 times with 5 ml PBS and cell pellets were re-suspended in 300 µl PBS.