Sentences with phrase «cell populations generated»

Dr. Sonntag studies this concept on the molecular and cellular level using a translational research approach that integrates the analysis of human material, such as postmortem brains, primary cell systems, and neural cell populations generated from patients» - or healthy individuals» - derived induced pluripotent stem cells (iPSC), or induced neurons (iNs), in combination with molecular, biochemistry, and lentivirus - mediated gene - engineering technologies.

Different clonal memory cell populations had different B cell or macrophage helper compositions that matched effector cell populations generated much earlier in the response.

Attempts to eliminate the tumor - initiating cell population generated by EMT are hampered by their increased resistance against most conventional cancer therapeutics.

Not exact matches

In the clearest possible case, the ANT - OAR cell would differ from a zygote on all of the parameters noted above: The ANT - OAR cell would have a pattern of gene expression that is clearly distinct from a zygote; it would generate a homogeneous population of cells rather than multiple cell types; it would undergo simple cleavage divisions and not produce any multicellular structures.

They observed that after allergic sensitization, some mouse strains generated large populations of MMC9 cells while other strains did not.

Frey and his colleagues made use of mathematical models to analyze the complex interplay between ecological factors and population dynamics, and were able to demonstrate that populations can indeed respond in a coordinated fashion to chemical information even when the signal molecules are generated by only a subset of the cells.

According to the authors, future studies should aim to improve the efficiency of the approach to generate a pure population of cells that closely mimic adult Leydig cells.

She said that a failure to maintain NFkB signals at this point, or even after memory T cells have been produced, leads to a loss of the population of memory T cells that have been generated after the infection.

Mycobacteria can generate daughter cells through asymmetric growth, resulting in genetically identical, but physiologically diverse, populations.

These results collectively suggest that neural stem cells need MSI1 to generate enough neurons for normal brain size, but the presence of MSI1 also increases the vulnerability of these cells to Zika infection, leading to the death of the population which ultimately results in microcephaly.

«In combination with recent advances in sorting cells on a biophysical basis, the biomechanical stemness markers we identified hold the potential to rapidly generate corneal transplants with highly enriched stem cell populations, which could one day translate into better outcomes for these patients.»

Interestingly, CD44 + / CD24 − population can be generated by induction of epithelial — mesenchymal transition (EMT) by treating breast epithelial cells with EMT - inducing agents such as TGF - β1 (6).

For isolated cell populations, SuperScript III was used to generate cDNA.

The selection strategy targeted cell populations expressing the inducible endothelial cell adhesion molecules, E-selectin and VCAM - 1, and proved successful in generating microvascular endothelial cell lines from a number of different organs.

Thus, our selection strategies, which were based on targeting endothelial populations that expressed both of these inducible glycoproteins, suggest that the endothelial cell lines generated in this study are most likely from this microcirculatory region.

The results of our limiting dilution analysis confirmed that the isolated CSCs (CD24 − / CD44 + / ESA +) population has significantly stronger ability of generating tumors compared with non-stem cell population (Table 1).

However, unlike HUVECs or other commercially available endothelial cell lines, the endothelial cells generated from the H - 2Kb - tsA58 mice retained their ability to mobilize these adhesion molecules despite undergoing numerous population doublings (currently 30 passages).

Within five to nine days we can generate virtually all the pure cell populations that we need.»

In this study, we used an in vivo strategy to determine if functional retinas could be generated from a defined population of pluripotent Xenopus laevis cells.

The ability to make pure populations of these cells within days rather than the weeks or months previously required is a key step toward clinically useful regenerative medicine — potentially allowing researchers to generate new beating heart cells to repair damage after a heart attack or to create cartilage or bone to reinvigorate creaky joints or heal from trauma.

The researchers also found that another population known as progenitor cells — differentiated daughter cells of stem cells — started to behave like stem cells: They began to live much longer than their usual lifespan of a few days, and they could also generate mini-intestines when grown outside of the body.

The ability to quickly generate purified populations of specialized precursor cells has opened new doors to further study.

Homogenous and functional population of keratinocytes and melanocytes derived from pluripotent stem cells were generated by designing a multi-step sequential protocol respecting the chronobiology of epidermis formation during human ontogenesis.

«The ability to generate pure populations of these cell types is very important for any kind of clinically important regenerative medicine,» said Loh, «as well as to develop a basic road map of human embryonic development.

The study of mice suggests that a high - fat diet drives a population boom of intestinal stem cells and also generates a pool of other cells that behave like stem cells — that is, they can reproduce themselves indefinitely and differentiate into other cell types.

«Often, we want to generate a particular cell population from stem cells for introduction into patients,» says co-senior author Young - sup Yoon, MD, PhD, professor of medicine (cardiology) and director of stem cell biology at Emory University School of Medicine.

His team also discovered how to generate functional hemangioblasts — a population of «ambulance» cells — from hESCs.

Today, analyzing and editing genomes, proteomes and metabolomes has become a standard for many model systems; imaging beyond the diffraction limit of light and new technologies for studying protein structures provide insights deeper than ever before; the characterization of large populations of cells or organisms brings unprecedented statistical power; and studying nearly all organisms of an ecosystems as a whole allows generating comprehensive models.

Starting with transplants of human oligodendrocytes in the late 1980s [40], and more recently with populations of human oligodendrocyte progenitor cells isolated from the developing or adult CNS, or from human embryonic stem cells, it has been possible to generate extensive myelination upon transplantation into spinal cord injury or into congenital mouse models of hypomyelination [41]--[48].

The Hematopoietic stem cell population resident in bone marrow is responsible for generating blood cells and immune cells.

We show that subpopulations of human astrocytes, generated by activation of different signaling pathways in the same population of human glial precursor cells, have markedly different effects when transplanted into the injured spinal cord.

We now show that astrocytes generated from the same population of human fetal glial precursor cells, by exposure to either bone morphogenetic protein (BMP) or ciliary neurotrophic factor (CNTF), promote widely divergent outcomes with respect to repairing the injured adult spinal cord.

Further challenges include the identification of signaling molecules that promote the generation of beneficial populations of astrocytes, identification of appropriate stem and / or progenitor cell populations that can be the source of such cells, and determination of whether there are situations in which it is more useful to transplant astrocytes themselves rather than to transplant stem or progenitor cells that might generate astrocytes in vivo in response to signals present in the host environment.

As a first step towards determining whether human glial progenitor cells (hGPCs) can generate functionally distinct astrocyte populations, we exposed embryonic hGPCs isolated from spinal cords of 9.5 week old abortuses to BMP or CNTF.

Thus while the majority of the V + population existing in ES cell cultures are indistinguishable from undifferentiated ES cells, we also observe differentiated cells expressing high levels of the Venus transgene (arrows in Figure 2B) that resemble the high - level Venus expressors generated in response to differentiation and that probably represent spontaneous PrEn differentiation.

Clinical trials are now underway that aim to see if the large populations of T regulatory cells generated in the thymus can be exploited to better protect against type 1 diabetes.

This approach involves first generating many monoclonal antibodies raised against «crude» tumor cell antigen populations — whole cells and cell membranes — and then screening for those that block the metastatic ability of the cell.

Despite remarkable improvement in the generation of hPSC - CMs, without purification, these protocols can only generate mixed cell populations including undifferentiated hPSCs or non-CMs, which may elicit adverse outcomes.

The researchers now hypothesize that microbes in the gut, where most of this pTreg cell population is switched on, may be responsible for generating these protective cells and thus protecting against the autoimmune attack on pancreatic beta cells that cause type 1 diabetes.

More than half of mammalian genes generate multiple messenger RNA isoforms that differ in their 3 ′ untranslated regions (3 ′ UTRs) and therefore in regulatory sequences, often associated with cell proliferation and cancer; however, the mechanisms coordinating alternative 3 ′ - UTR processing for specific mRNA populations remain poorly defined.

There are populations of cells that divide in the mature brain, and the knowledge we gain from the in vitro studies could help influence how one might be able to manipulate and use that newly generated population of cells in the brain in more therapeutic ways.

Against common wisdom even the adult and aging brain can generate new neurons from a population of resident stem cells but it does so only in two privileged regions and on a minute scale.

The low density culture protocol helps in generating larger number of CD133 + endothelial population from initially fewer CD133 + endothelial progenitor cells; EPCs (Figure S1).

Among other projects, according to Pop!Tech, she's designing a cheap wind turbine capable of generating enough electricity to charge a cell phone or power LED lighting in Guatemala, where 75 percent of the population lives below the poverty line.