Sentences with phrase «cell populations using»

Flow cytometry is a powerful technique that allows researchers to examine multiple proteins on cell populations using fluorescently labeled antibodies.

RNA was extracted from different cell populations using Trizol ™ (Invitrogen) and precipitated with isopropanol.

We present an in - depth characterization of human T cell populations using a panel of ~ 80 antibodies revealing complexity not previously observed by scRNA - seq alone.

Research Focus: I am highly trained in producing and purifying monoclonal antibodies from Hybridoma cells, performing ELISA assays, preparing primary cells from organs and analyzing cell populations using FlowJo Cytometry data analysis software, isolating genomic DNA from animal tissues, maintaining and screening numerous transgenic and knock out mice lines by PCR and FACS.

Isolate RNA from each collected cell population using TRI reagent following manufacturer's instructions.

They hypothesized that, if they labeled the parent cell population using the library, propagated it, separated it into roughly identical replicate groups, and treated these groups with the same drug regimen, they would find one of two outcomes (see schematic depiction below):

Not exact matches

(i) a woman's right to choose; (ii) teaching evolution in school; (iii) medical immunization of teen girls against HPV; (iv) assisted suicide; (v) gay marriage; (vi) my right to view art and theatre deemed «offensive,» «blasphemous» or «obscene» by theists (vii) basic $ ex education for older school children; (viii) treating drug abuse as principally a medical issue; (xi) population control; (x) buying alcohol on a Sunday; (xi) use of condoms and other contraceptives (xii) stem cell research.

(i) a woman's right to an abortion; (iii) medical immunization of teen girls (and boys) against HPV; (iv) assisted suicide; (vi) gay marriage; (vii) my right to view art and theatre deemed «offensive,» «blasphemous» or «obscene» Catholics; (viii) basic $ ex education for older school children; (ix) treating drug abuse as principally a medical issue; (x) population control; (xi) buying alcohol on a Sunday in many places; (xii) use of condoms and other contraceptives; (xiii) embryonic stem cell research; (xiv) little 10 year - old boys joining organizations such as the Boy Scouts of America, regardless of the religious views of their parents; and (xv) gays being allowed to serve openly in the military.

With 212 million cell phone users in the US — about 75 % of the population — the potential for using mobile phones («mo - phos,» as the kids call them) as a tool to grow your organization's visibility and spread your message is enormous.

The government has been forced to introduce emergency measures to tackle a bursting jail population, which includes using police cells from today.

Instead, the team used functional magnetic resonance imaging (fMRI) to detect populations of cells that seemed to fire as the volunteers played a virtual reality game in which they had to replace an object in the location where they originally found it.

She works to isolate populations of these cells, discover how the body regulates them, and understand how they can be used to treat disease.

Many disease - causing bacteria use virulence factors to invade and destroy cells, and they often use quorum sensing to activate these virulence factors when their population reaches a critical threshold to mount a devastating attack.

We don't need to use a cocktail of small molecules, growth factors or other supplements to create a population of bone cells from human pluripotent stem cells like induced pluripotent stem cells,» Varghese said.

Although the new JNCI study did not analyze individual cell phone usage — preferring instead to take a broader look at the population at large — other research has examined subgroups of cell phone users to try to discern different trends among more frequent use.

Human epidermal equivalents representing different types of skin could also be grown, depending on the source of the stem cells used, and could thus be tailored to study a range of skin conditions and sensitivities in different populations.»

A University of Wyoming faculty member led a research team that discovered a certain type of soil bacteria can use their social behavior of outer membrane exchange (OME) to repair damaged cells and improve the fitness of the bacteria population as a whole.

Next, they used a technique called fluorescence - activated cell sorting to separate out the newly differentiated retinal ganglion cells from a mixture of different cells into a highly purified cell population for study.

By using these and other high - tech tools, the researchers homed in on a unique population of T - cells that are highly prevalent in the joints of rheumatoid arthritis patients.

Saatchi, which is owned by France's Publicis Groupe, SA, chose LifeStraw over a field of competitors that included a reusable controller to improve the distribution of IV fluids, a collapsible wheel that can be folded down for easier storage when not in use on bicycles or wheelchairs, an energy - efficient laptop designed for children in developing countries, a 3 - D display that uses special optics and software to project a hologramlike image of patient anatomy for cancer treatment, an inkjet printing system for fabricating tissue scaffolds on which cells can be grown, a visual prosthesis for bypassing a diseased or damaged eye and sending signals directly to the brain, books with embedded sound tracks to help educate illiterate adults on health issues, a phone that provides telecommunications coverage to poor rural populations in developing countries, and a brain - computer interface designed to help paralyzed people communicate via neural signals.

Next steps include examining whether the viral infections play a role in controlling the population of toxic algae and continued studies on the nutrients these cells use to grow.

Researchers in this study used budding yeast, creating populations of cells with more than 10 million different randomised genomes, to investigate how genetic diversity affected resistance.

At one level, optogenetics is nothing more than using light to control a targeted population of cells.

Frey and his colleagues made use of mathematical models to analyze the complex interplay between ecological factors and population dynamics, and were able to demonstrate that populations can indeed respond in a coordinated fashion to chemical information even when the signal molecules are generated by only a subset of the cells.

By varying the compositions of lipids, cues, and diffusible factors in the scaffolds, we engineered a very versatile and flexible platform that can be used to amplify specific T cell populations from blood samples, and that could be deployed in existing therapies such as CAR - T cell therapies,» said Mooney, Ph.D., a Core Faculty member at the Wyss Institute and leader of its Immunomaterials Platform.

For instance, researchers often use so - called «gene guns» to fire particles coated with strands of DNA known as plasmids at large populations of cells.

Using only the DNA from sloughed - off cells floating in the ocean, scientists have been able to determine the population size and genetic properties of one of the world's largest and most mysterious animals: the whale shark.

Researchers take advantage of this feature to obtain populations of synchronized bacteria that can be used to study specific cell cycle processes.

Using fluorescent markers, Fuchs and her colleagues isolated two distinct populations of stem cells from mouse skin.

Techniques enabling the isolation of specific cell populations have advanced our ability to identify and characterize niche cell types which may be used to replace or engineer cells / tissue.

The use of cell surface markers to isolate specific cell populations is one common method for separating cells; however, isolating live cells based on their RNA expression is a powerful new way enabling the study of small cell niches in nongenetically modified animal models and human tissue.

The code that a monkey's brain uses to represent faces relies not on groups of nerve cells tuned to specific faces — as has been previously proposed — but on a population of about 200 cells that code for different sets of facial characteristics.

«The surprising result is that even though only a very localized population of cells is used in the brain - computer interface, the brain recruits many other areas that aren't directly involved to get the job done.»

Luckily, a mouse model already existed that allowed the team to take advantage of this gene expression and use it to compare the two cell populations.

Their recent study, which appears as the cover article in the May issue of Cancer Research, shows that mathematical models can be used to predict how different tumor cell populations interact with each other and respond to a changing environment.

Using sensitive live cell imaging, the team noted that endocytic waves initiated by clathrin emerge in some cell populations.

The DKFZ researchers conducted this study in collaboration with Epiontis, a Berlin - based company that specializes in the epigenetic tests that were used to determine the ratio of the various T cell populations.

Using molecular tools, he and his colleagues conducted a study, published in Science in 2012, which showed that a large population of fungal cells — called a mycobiome — lives in the gut and likely plays a role in IBD.

In this work, we use the DNA of living cell populations as genomic «tape recorders» for the analog and distributed recording of long - term event histories.

We describe a scalable platform that uses genomic DNA for analog, rewritable, and flexible memory distributed across living cell populations.

By matching the microsatellite signature in a population of cells to those of known cells in the FACC - 29, a researcher can validate the identity of the cells used in research.

A Ludwig Cancer Research study published online September 14th in Nature reports a novel technique to map specific chemical (or «epigenetic») modifications made to the protein packaging of DNA using a small population of cells.

For isolated cell populations, SuperScript III was used to generate cDNA.

The team used a combination of experiments to zero in on the molecular activity of melittin at the «minimal inhibitory concentration» (MIC), the lowest concentration that's been shown to slow the growth of target cell populations.

The experimental plan includes a fine - grained analysis of the different cell populations within these precious tissues using cutting - edge single - cell genomics methods.

In this study, we used an in vivo strategy to determine if functional retinas could be generated from a defined population of pluripotent Xenopus laevis cells.

The capacity of each cell population to induce apoptosis was tested using a 24 - hr three - color apoptosis assay.

The present findings are derived from studies using CD4KO mice and thus, raise the question as to whether the CD4 + T cell - independent immune mechanisms in CD4 KO mice differ from those involved in corneal allograft rejection in wild - type mice whose CD4 + T cells population have been depleted with monoclonal antibodies.

Dr. Sonntag studies this concept on the molecular and cellular level using a translational research approach that integrates the analysis of human material, such as postmortem brains, primary cell systems, and neural cell populations generated from patients» - or healthy individuals» - derived induced pluripotent stem cells (iPSC), or induced neurons (iNs), in combination with molecular, biochemistry, and lentivirus - mediated gene - engineering technologies.

ClioCell has the ability to selectively remove inhibitory or toxic dead cells using a simple and rapid procedure results in radically improved cell populations