Sentences with phrase «cell reprogramming factor»

«Stem cell reprogramming factor controls change in cellular energy generation: Research reveals role for KLF4 protein in instructing cells to undergo metabolic changes while acquiring stem cell characteristics.»

The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming factors and wait for cells to de-differentiate.

Not exact matches

Using patient's own tissue and specific combination of reprogramming factors, skin fibroblasts are successfully converted to cell type that... Continue reading Breakthrough Device Looks to Heal Organs

It is far more likely, however, that the egg - cell cytoplasm with its stripping factor will reprogram all the genetic material including the alterations made in the donor nucleus that were intended to prevent the creation of the zygote.

Reprogramming involves inducing the expression of four factors, called Yamanaka factors, in cells.

The team induced expression of Yamanaka factors in all cells of the organism using their partial reprogramming approach.

While the process usually proceeds in a one - way direction, artificially inducing the activity of key transcription factors can reprogram differentiated cells back into a stem - like state, a discovery honored with the 2012 Nobel prize.

Testing each of these factors for their ability to return differentiated tumor cells to a stem - like state, identified a combination of four — POU3F2, SOX2, SALL2 and OLIG2 — that was able to reprogram differentiated tumor cells back into glioblastoma stem cells, both in vitro and in an animal model.

The study team removed fibroblasts (skin cells) from DBA patients, and in cell cultures, using proteins called transcription factors, reprogrammed the cells into iPSCs.

«By identifying the areas of the genome that are directly involved in the reprogramming, we have also identified an important factor in the process — the gene regulatory protein KLF11 (Kruppel Like Factor - 11), which is found in all fat cells, and we have shown that it is required for the reprogramming to take place.&factor in the process — the gene regulatory protein KLF11 (Kruppel Like Factor - 11), which is found in all fat cells, and we have shown that it is required for the reprogramming to take place.&Factor - 11), which is found in all fat cells, and we have shown that it is required for the reprogramming to take place.»

These factors guide a reprogramming process that reverts the cells to an embryonic state, in which they have the potential to become virtually any type of cell.

«Maybe at the one - cell or two - cell stage,» Eggan and his colleagues reasoned, «there's still some of that stuff in there...» And if they picked the right moment of cell division, when these powerful reprogramming factors were still floating around in the periphery of the cell, they might be able to use drugs to temporarily freeze the cell in the middle of division, stick in the needle of a micromanipulator to suck out the embryonic DNA, squirt in DNA from an adult animal, and then kick - start the process of reprogramming — hours, perhaps even days after an egg had been fertilized.

But until now no one has been able to identify the factors affecting the reprogramming of precursor fat cells or establish the rapidity at which cells are reprogrammed.

The stem cell transcription factor SOX2, which is critical for stem cells, cell reprogramming, and brain development, also is activated in astrocytes in the adult brain.

KLF4 together with other reprogramming transcription factors is used in the lab to force the expression of genes in somatic cells (adult non-germline cells) in the development of iPSCs.

We have now discovered that this factor also acts as a catalyst when reprogramming adult cells into iPS,» explains Thomas Graf, senior group leader at the CRG and ICREA research professor.

One week after a breakthrough finding, scientists report they can reprogram human skin cells to behave like embryonic stem cells without a growth factor known to cause cancer

They developed transgenic mice in which a specific drug can turn on the four reprogramming factors in all the animals» cells.

«Researchers identify key factor for reprogramming adult cells into stem cells.»

However, these problems associated with histocompatibility may be solved using autologous donor adult stem cells, therapeutic cloning, stem cell banks or more recently by reprogramming of somatic cells with defined factors (e.g. induced pluripotent stem cells).

«Work in other organs has shown that reprogramming cells is rarely accomplished by manipulating a single factor,» Zuo said.

Transcription factors orchestrate dynamic interplay between genome topology and gene regulation during cell reprogramming.

So what we think is that probably in many cells in this section, all cells, the chromatin is encountered in a specific state, and in order to render the cell is permissive to reprogramming, you have to overcome these certain epigenetic modifications that block, for example, the binding of Ascl1 to its target chains, or the binding of other transcription factors to its target chains, then this way interfere with the possibility of reprogramming.

First discovered a series of small molecules and conditions that can replace reprogramming transcription factors and enhance reprogramming efficiency in generating iPS cells from somatic cells.

But without any neuromorphology, thus we tested co-expression of SOX2, another transcription factor, which figures prominently amongst the Yamanaka reprogramming cocktail, but it's also well - known for its role in neuro stem cells.

Factors that drive neuronal specification in the developing CNS, are good candidates for reprogramming other somatic cells into neurons in vitro and perhaps also in vivo.

The researchers identified 24 potential reprogramming factors — proteins that control whether other genes in the cell are turned on or off.

It has recently been demonstrated that mouse and human fibroblasts can be reprogrammed into an embryonic stem cell - like state by introducing combinations of four transcription factors.

These reprogrammed iPS cells are usually created by expressing four genes, called Yamanaka factors, normally unexpressed (or expressed at very low levels) in adult cells.

Using a unique chemical cocktail of small molecules and other reprogramming factors, Dr. Ding never lets the cells enter the pluripotent state.

We have identified a number of key transcription factors that are deregulated during this process, and we are using this information to investigate mechanisms by which differentiation can be reprogrammed in tumour cells.

Several different transcription factors have been found that can directly reprogram one type of blood cell into another.

More is not always better in reprogramming, as researchers show that a set of factors which reprogram cells with low efficiency are developmentally superior to other more «efficient» reprogramming cocktails.

Non cell - autonomous reprogramming of adult ocular progenitors: generation of pluripotent stem cells without exogenous transcription factors.

Additionally, the originally generated iPS cells contained DNA randomly inserted into the genome from the retrovirus system, although researchers have since made new forms of iPS cells using non-integrating systems and have also improved delivery of the reprogramming factors to the cells.

This new paper demonstrates that one of the more controversial reprogramming factors (c - myc) is not absolutely necessary to produce iPS cells which gives us hope that methods may be developed soon that produce cells which can be used in human patients.

However, there is still much work to be done to prove that the cells are safe particularly since even this newer method still uses retroviruses to tranfer the reprogramming factors.»

The research published in the journal Nature Biotechnology shed more light on which genetic factors are critical in the reprogramming of adult skin cells to become other types of cell.

This is beautiful science continuing directly from lasts week's publications to identify genes or factors critical for reprogramming a differentiated adult cell back into a pluripotent stem cell.

The factors were actually added to, not deleted from, the original adult cells that were reprogrammed into iPSCs.

To achieve efficient and synchronous reprogramming, the authors expressed reprogramming factors (OSKM) in mouse bone marrow - derived pre-B cells exposed to the C / EBPα transcription factor, leading to nearly 100 % reprogramming within 4 to 8 days.

Using NPCs, Silva et al. delineated two phases in the reprogramming process (pre-pluripotency and ground state pluripotency) and demonstrated that dual inhibition (2i) of mitogen - activated protein kinase (MAKP) signaling and glycogen synthase kinase - 3 (GSK3) signaling combined with the self - renewal cytokine leukemia inhibitory factor (LIF) promotes pre-pluripotent cells to ground state pluripotency [19].

The scientists also used the chemicals to improve direct cardiac reprogramming of human cells, which is a more complicated process that requires additional factors.

Using a technique known as transcription factor reprogramming, first described by the Japanese stem - cell pioneer Shinya Yamanaka and his colleagues in 2007, Ku «rebooted» the skin cells» genetic machinery to turn them into stem cells.

Since murine NSCs already express high levels of Sox2 [14]--[16], it was tested whether these cells could be reprogrammed into iPS cells by only a few critical factors.

Here we have identified another source of human cells that can be efficiently reprogrammed into human iPS cells by the ectopic exposure of only two factors, namely OCT3 / 4 and KLF4.

The crux of the discovery, published online Tuesday by the journals Cell and Science, is a «direct reprogramming» technique that adds a cocktail of four genetic factors to run - of - the - mill human skin cells.

In two - factor reprogramming experiments, 80 % of cells were infected with both OCT3 / 4 and KLF4.

Since murine NSCs have been «primed» with several of the factors originally discovered to reprogram fibroblasts into iPS cells, they represent an attractive source of starting material for iPS cell induction studies.

In this study, we develop a reproducible protocol for efficient reprogramming mouse neural progenitor cells (NPCs) on human foreskin fibroblast (HFF) cells via retroviral transfer of human transcriptional factors OCT4 / SOX2 / KLF4 / C - MYC.