Sentences with phrase «cell sequencing from»

Information obtained via single - cell sequencing from cells sampled from each core indicate the likely location of a cancerous tumor (dotted lines encompassing cores 2, 3 and 6).

Not exact matches

Researcher Aviv Regev's «lab has gone from looking at 18 cells at a time to sequencing RNA from hundreds of thousands.»

From which it follows, ultimately, that the self must be regarded as a series of momentary selves the unity of each of which can only be regarded as the unity of a complex set of what I will refer to as «interrelated event - cells,» while the unity of the more general sense of «person» or «self» can only be regarded as the unity of a sequence or succession.

People of every nation, color, language, belief, and condition are now known to possess in their body cells trait factors drawn by an inconceivably complex sequence of intercombinations from a common «gene pool.»

The evolutionary sequence from protons, molecules, cells, plants and animals to people would be interpreted as an increase in complexity of experience and degree of self - determination.

@Chad: I. Mutations in DNA sequences generally occur through one of two processes: A. Environmental factors: DNA damage from environmental agents such as ultraviolet light (sunshine), nuclear radiation or certain chemicals B. Mistakes that occur when a cell copies its DNA in preparation for cell division.

To determine how the cells switch from one type to another, they took three human uterine carcinosarcoma samples and sequenced the genomes of cells in two parts of each tumor: the carcinoma and sarcoma components.

Using a relatively new technology called CRISPR / Cas9, they managed to accurately cut out specific DNA sequences from leukemia cells.

These modifications represent a layer of information that can be passed from a parent cell to a daughter cell but is not encoded in the DNA sequence.

But to coax them down a specific path in the lab requires recreating the precise sequence and timing of environmental cues in the womb — the signals from proteins and hormones that tell cells what kind of tissue to become.

«However, because SIF - seq only requires DNA sequence from a mammal and can be used in a variety of cell types, it should be possible to compare the neuronal enhancers present in a large genomic region from human to the neuronal enhancers present in the orthologous chimpanzee region.

Blackburn and Szostak determined that it was a specific DNA sequence in the telomeres that kept chromosomes from fraying whenever they were copied when a cell splits in two.

Biotech experts point out that, aside from some clever watermarks the researchers stuck into the genetic sequence, the synthetic cells are identical to a natural species.

To start, she used a cotton swab to get a sample of cells from her cheek, boiled them in a test tube in her kitchen to free the DNA, then added primers, nucleic acids that mark the part of the sequence.

An analysis of the HPV16 genome from 5,570 human cell and tissue samples revealed that the virus actually consists of thousands of unique genomes, such that infected women living in the same region often have different HPV16 sequences and variable risks to cancer.

To address this gap in knowledge, Mirabello and Schiffman teamed up with co-senior author Robert Burk of Albert Einstein College of Medicine to sequence the whole genomes of 5,570 HPV16 - infected cell and tissue samples from women around the world and to identify associations between HPV16 genetic variants and the risk of cervical precancer and cancer.

Through a variety of «high tech» approaches, including the isolation of monoclonal antibodies from single B cells and ultra-deep sequencing of shifting viral populations over more than three years of infection, the researchers studied one woman who developed potent broadly neutralizing antibodies.

Project members also catalogued sequences that mark areas where DNA unwinds from the round histone proteins that maintain the shape of chromosomes, allowing the cell's transcription machinery to activate genes in those areas.

Bar coding typically involves sequencing a few short segments of animal DNA from the mitochondria, the mini-organs that produce energy within every cell.

Lu's team will extract immune cells called T cells from the blood of the enrolled patients, and then use CRISPR — Cas9 technology — which pairs a molecular guide able to identify specific genetic sequences on a chromosome with an enzyme that can snip the chromosome at that spot — to knock out a gene in the cells.

To more accurately reflect the mechanisms driving oligodendrogliomas, the researchers used RNA sequencing to study directly, on a single - cell level, gene expression in samples from six early - stage human tumors.

HPV is detected using a DNA analysis that looks for characteristic sequences from the 13 high risk strains of the virus in the cervical cell sample.

In germline cells PIWI proteins silence the RNA from jumping genes by cutting them in sequences of ~ 30 nucleotides that will become piRNAs.

Though these barcodes are synthesized differently from 10x Genomics» RNA - sequencing barcodes, their purpose is similar: to link each cell's amplified genome pieces to a unique identifying sequence.

The need to compare multiple cells» DNA sequences to distinguish genuine genetic variants from artifacts limits some of the potential of single - cell sequencing.

Mitochondria are the cell's energy - generating organelles, and they have their own DNA, which is distinct from the much longer DNA sequence that resides in the cell's nucleus.

In the next phase of the study, researchers will genetically sequence tumor cells from at least 500 patients and follow the course of their disease.

In this study, rather than studying one iPS cell line, the researchers derived and sequenced 10 iPS cell clones from each patient tissue sample to get a better understanding of mitochondrial DNA mutation rates.

When they sequenced the iPS cell lines, they found higher numbers of mitochondrial DNA mutations, particularly in cells from patients older than 60.

Cell - free genomic DNA isolated from human cells was cleaved with preassembled, recombinant Cpf1 RNPs and subjected to whole - genome sequencing.

Current genome sequencing techniques involve the chemical disintegration of samples of red blood cells from infected patients to obtain parasite DNA, which are then sequenced.

By comparing proteomic and RNA - sequencing data from people on different exercise programs, the researchers found evidence that exercise encourages the cell to make more RNA copies of genes coding for mitochondrial proteins and proteins responsible for muscle growth.

Focusing on DNA from chloroplasts and mitochondria — energy - producing structures in cells that have their own genomes — the researchers produced 49,000 genetic sequences.

Researchers sequenced ancient DNA from the mitochondria — tiny energy factories inside cells — from a Neandertal who lived about 100,000 years ago in southwest Germany.

The team integrated three, complementary gene sequencing approaches to look for mutations in tumor cells from SS patients: whole - genome sequencing in six subjects, sequencing of all protein - coding regions (exomes) in 66 subjects, and comparing variation in the number of copies of all genes across the genome in 80 subjects.

Moreover, because they had an idea of what cells the genes came from and what kinds of gene sequences produce what kinds of proteins, they were able to draw some conclusions about what all those genes are good for.

Quintana - Murci and colleagues used RNA - sequencing to characterize the way that immune cells, known as primary monocytes, derived from 200 people of self - reported African or European ancestry would respond to attack by a bacteria or a virus.

Professor Gianni Liti, a senior author on the paper from the Institute for Research on Cancer and Ageing, Nice, said: «We were able to study the evolution in time by combining genome sequences of the cell populations and tracking the growth characteristics of the yeast cells.

A multicenter team of researchers reports that a full genomic analysis of tumor samples from a small number of people who died of pancreatic cancer suggests that chemical changes to DNA that do not affect the DNA sequence itself yet control how it operates confer survival advantages on subsets of pancreatic cancer cells.

Co-first author Alice Eunjung Lee, PhD, from the lab of Peter Park, PhD, at the Center for Biomedical Informatics at Harvard Medical School, developed the study's retrotransposon analysis tool, which detects somatic retrotransposon mutations in single - cell sequencing data.

The genomes of these 377 bacterial isolates, plus an additional 107 single bacterial cells from roots of A. thaliana, were then sequenced, assembled, and annotated at the JGI.

If the miRNA recognition sequence binds to miRNA expressed in the desired cells, the expression of the regulatory protein is suppressed, thus distinguishing the cell type from others that do not contain the miRNA and express the protein.

DNA sequences were once thought to be identical from cell to cell, but it's increasingly understood that mutations can arise during brain development that affect only certain groups of brain cells.

The DNA analysis used in this study focused on mitochondrial DNA, which is easier to recover from fossils than the DNA in chromosomes, because each cell has thousands of copies of the relatively short mitochondrial DNA sequence.

Here, Patil et al. have carried out single RNA - seq and sequenced the T cell receptors (TCRs) of CD4 + T cells from human blood to identify precursors that give rise to CD4 - CTLs.

In the developing brain, stem cells in the heart of the cortex produce neurons in sequence from the inner layer outwards.

The SC3 tool was then used to analyse single - cell RNA - sequence data from two patients diagnosed with myeloproliferative neoplasm (MPN) blood cancers.

Using single - cell RNA sequencing, the team was able to profile molecular features and metabolic activity of individual beta cells to determine how dividing beta cells differ from non-dividing cells.

The team used a statistical model of T cell receptor formation to analyze receptor DNA sequences in blood samples from adult pairs of identical twins.

Dr Vladimir Yu Kiselev, first author from the Sanger Institute, said «We created the new SC3 tool to analyse complex single - cell RNA - sequence data, and showed that it is more robust and accurate than existing methods at grouping cells.