Sentences with phrase «cell sequencing on»

He also recommends performing standard bulk - cell sequencing on a portion of the sample, to provide a basis for comparison with the single - cell DNA data.

Not exact matches

«We couldn't have figured out what was happening without the expertise of our collaborators at U.C. Davis, who determined the genetic sequence of every single cell marker on the cells in the lymph nodes,» says Miller.

Zhang's group is moving beyond bulk sequencing of tumor samples and is now conducting genomics on single cells.

The man responsible for one of the original sequences of the human genome as well as the team that brought you the first living cell running on human - made DNA now hopes to harness algae to make everything humanity needs.

Umen focused on the «mating locus» (MT)-- the genetic sequence behind the making of sex cells.

It's well established, of course, that environmental effects like radiation, which alter the genetic sequences in a sex cell's DNA, can leave a mark on subsequent generations.

Lu's team will extract immune cells called T cells from the blood of the enrolled patients, and then use CRISPR — Cas9 technology — which pairs a molecular guide able to identify specific genetic sequences on a chromosome with an enzyme that can snip the chromosome at that spot — to knock out a gene in the cells.

To more accurately reflect the mechanisms driving oligodendrogliomas, the researchers used RNA sequencing to study directly, on a single - cell level, gene expression in samples from six early - stage human tumors.

They specifically studied the length of telomeres (repeated DNA sequences) on the ends of chromosomes in leukocytes (white blood cells); the protective caps are believed to be markers of biological aging, because they shrink over time.

Hindson adds that even seemingly simple issues, such as how the cells are isolated and stored before sequencing, can have a major impact on the quality of the data.

The two proteins have different biochemical properties and recognize different DNA sequences, so these properties create more options for gene - editing,» said Dr. Olson, who holds the Pogue Distinguished Chair in Research on Cardiac Birth Defects, the Robert A. Welch Distinguished Chair in Science, and the Annie and Willie Nelson Professorship in Stem Cell Research.

That's why most single - cell DNA sequencing now relies on multiple displacement amplification (MDA).

In a recent proof - of - concept experiment, Abate and his colleagues performed single - cell genomic sequencing on a sample of seawater.

Moderna is also doing animal safety tests of a personalized cancer vaccine that would code for immune - activating proteins unique to a person's cancer cells, based on genetic sequencing of their tumor.

One aspect of gene regulation involves enzymes placing chemical tags or modifications on histone proteins — which control a cell's access to the DNA sequences that make up a gene.

By comparing proteomic and RNA - sequencing data from people on different exercise programs, the researchers found evidence that exercise encourages the cell to make more RNA copies of genes coding for mitochondrial proteins and proteins responsible for muscle growth.

Focusing on DNA from chloroplasts and mitochondria — energy - producing structures in cells that have their own genomes — the researchers produced 49,000 genetic sequences.

Our current concept of the origin of the eukaryotic cell is in flux, however, and an evolutionary sequence that appears simple when conceptualized on a phylogenetic tree diagram may be far more complex and interesting in reality.

To deliver the healthy gene, the team inserted it into an engineered virus called adeno - associated virus 1, or AAV1, together with a promoter — a genetic sequence that turns the gene on only in certain sensory cells of the inner ear known as hair cells.

Professor Gianni Liti, a senior author on the paper from the Institute for Research on Cancer and Ageing, Nice, said: «We were able to study the evolution in time by combining genome sequences of the cell populations and tracking the growth characteristics of the yeast cells.

A multicenter team of researchers reports that a full genomic analysis of tumor samples from a small number of people who died of pancreatic cancer suggests that chemical changes to DNA that do not affect the DNA sequence itself yet control how it operates confer survival advantages on subsets of pancreatic cancer cells.

«Previous techniques that have been used to investigate DNA control sequences usually rely on sorting cells one by one and measuring gene activity in each of them,» says Dr Eva Yus, lead author of the paper.

The DNA analysis used in this study focused on mitochondrial DNA, which is easier to recover from fossils than the DNA in chromosomes, because each cell has thousands of copies of the relatively short mitochondrial DNA sequence.

The link to viral mimicry came when Cantor's group found that the same variant sequence is indeed present on corneal cells and also in a herpesvirus protein called UL6.

Relative to reactions on naked DNA, there are changes (protections and enhancements) in the reactivity of guanine residues to dimethyl sulfate within the enhancer sequence in myeloma, B, and early B cells, whereas virtually no alterations appear in cells of non-B lineage.

Dr Martin Hemberg, lead author on the paper from the Wellcome Trust Sanger Institute, said: «It has been difficult to fully exploit single - cell RNA - sequence data due to the current lack of computational methods for analysing them.

Connected at UNU - BIOLAC workshops in Montevideo, Uruguayan chemistry professor Francisco Carrau and scientist Massimo Delledonne of Italy recently collaborated on sequencing the Tannat grape, pressings of which, thanks partly to its many seeds, produces the largest concentration of tannins — an anti-oxidant that combats the ageing of cells.

As part of ENCODE, 32 institutions did computer analyses, biochemical tests, and sequencing studies on 147 cell types — six fairly extensively — to find out what each of the genome's 3 billion bases does.

Surprisingly, they found that although the patterns of gene expression — as shown by the RNA sequencing — differed between the hepatocellular carcinomas and the liver cancers with biliary phenotype and depended on the histological type, the overall pattern of mutations in the cells was actually similar between the tumors — of either type — that had emerged in patients who had had infections with either hepatitis C or B, and were different in patients without such infections.

If the DNA sequences don't match, the cells float on by.

After the sorting, we performed whole genome amplification on individual aggregates then sequenced the 16S rRNA marker genes to identify cells comprising the aggregate.

«If we could predict the final 3D structures of proteins from their genetic sequence, we would be one step closer to understanding the cell on a molecular level and curing disease,» says co-author Gevorg Grigoryan, an assistant professor of computer science at Dartmouth.

The result is a mixture of background sequences that record time and signal sequences that change depending on the cell's environment.

The device has made it possible for researchers to study bacteria and viruses in the field, but its high error - rate and large sequencing gaps have, until now, limited its use on human cells with their billions of nucleotides.

Yet, «these studies using HeLa cells had to rely on information from the «reference» sequence produced by the Human Genome Project, even though there was evidence that the genomes of HeLa cell lines were probably quite different,» said Lars Steinmetz, Ph.D., who led the G3 study.

Now, Skehel, along with colleagues at Harvard, Yale, and Emory University in Atlanta, Georgia, has used that sequence to build the virus's hemagglutinin (HA)-- a protein that latches onto receptors on the host cell surface — and determine its structure.

To determine the genetic basis for his exceptional response, researchers at the Dana - Farber Cancer Institute, Stanford University, Brigham and Women's Hospital and elsewhere performed targeted and whole - exome sequencing on his leukemic cells.

Using an unsupervised learning algorithm, the team was able to convert the T - cell receptor sequencing data into numeric distances based on similarities in the receptor sequences and cluster them by functional specificity.

Now, researchers writing in the Cell Press journal Cell Reports on February 12th have sequenced the complete genome of one wild - caught individual, and they say that it has already told them more than they had anticipated about the butterflies and some of their most intriguing features.

A new technique for sequencing the RNA in a single cell has been revolutionary, Ngai said, allowing researchers to trace which stem cells in a densely packed tissue become specialized, based on the mRNA present in the cell, which indicates which genes are being expressed.

The Johns Hopkins team tested ImmunoMap's ability to correlate immune responses on receptor sequencing data from T - cells in the tumors of 34 patients with cancer enrolled in a nationwide clinical trial of the immunotherapy drug nivolumab.

Dr. Montelaro and his colleagues found that a particular sequence of amino acids on the tail end of HIV allow the virus to «punch into» and infect cells.

The team then performed comprehensive RNA sequencing on the blood samples followed by computational data analyses to determine what genes and cells were activated and driving these allergic reactions.

The FACC - 29 library depends on microsatellite «signatures» that can be used to identify short segments of a cell's genetic sequence, in this case developed by the laboratory of study co-author, Dawn L. Duval, PhD, also working at the FACC.

To see if any of these lay in genetic sequences on which the p53 protein acts, the investigators used data from several lines of healthy and cancerous cells subjected to various p53 - activating treatments.

An article published on June 26th in PLOS Pathogens now reports that a mini antibody called 3D8 scFv can degrade (or chew up) viral DNA and RNA regardless of specific sequences and protect mammalian cells and genetically manipulated mice against different viruses.

On 11 March, the European Molecular Biology Laboratory (EMBL) issued a press release proudly announcing that a research team there had deciphered much of the genetic sequence of one of the most widely used cell lines in cancer studies and had made the information available publicly.

The vectors also contained DNA bar codes, sequences that the researchers could look for later to determine which small RNAs were having a big effect on a cell's behavior.

Western Pygmies I love population genetics for its ability to peer back into human history through the medium of DNA's ATCGs.One of the stars of this discipline is Sarah Tishkoff, a standout in African genetics, someone who will readily haul a centrifuge into the bush in Cameroon.Tishkoff of the University of Pennsylvania is lead author on a paper published online July 26 in Cell that details whole - genome sequencing of five individuals each from three extant hunter - gatherer groups — the Pygmies of Cameroon as well as the Hadza and the Sandawe of Tanzania.

Pharmaceutical researchers are now on the lookout for molecules that have these recognition sequences in the fitting spatial structure, allowing them to trigger the same cell reactions.