A new study used single
cell sequencing technology to provide previously...
The Korbel group combines experimental and computational approaches, including single -
cell sequencing technology, to unravel determinants and consequences of germline and somatic genetic variation.
This includes the development of advanced laboratory tools such as CRISPR genome - editing and single -
cell sequencing technologies.
Not exact matches
That's attached to the progress of
sequencing technology, the ability to edit
cells, and other gene editing approaches have been transformative in the immunotherapy world in recent years.
Using advances in genomic
sequencing, the human microbiome, proteomics, informatics, computing, and
cell therapy
technologies, HLI is building the world's most comprehensive database of human genotypes and phenotypes as a basis for a variety of commercialization opportunities to help solve aging related disease and human biological decline.
Using a relatively new
technology called CRISPR / Cas9, they managed to accurately cut out specific DNA
sequences from leukemia
cells.
Single -
cell DNA and RNA
sequencing technologies are entering the marketplace.
The kit also preserves the single - nucleotide variants (SNVs) and SNV frequencies across different
sequencing technologies and allows the amplification of
cell - free DNA to levels that permit
sequencing.
Lu's team will extract immune
cells called T
cells from the blood of the enrolled patients, and then use CRISPR — Cas9
technology — which pairs a molecular guide able to identify specific genetic
sequences on a chromosome with an enzyme that can snip the chromosome at that spot — to knock out a gene in the
cells.
This family tree captures just a sampling of single -
cell and single - nucleus RNA
sequencing technologies that have burst onto the scene over the last eight years.
Using next - generation
sequencing technologies that were previously unavailable, Michael Gardner, a graduate research assistant, and Jianying Wang, a senior research associate in Mitchum's lab, made a remarkable new discovery — nematodes possess the ability to produce a second type of peptide that can effectively «take over» plant stem
cells that are used to create vital pathways for the delivery of nutrients throughout the plant.
The researchers analysed the genetic composition of myocardial
cells using state - of - the - art gene
sequencing technology.
In recent years advances in
sequencing technology have enabled detailed investigations of the RNA molecules that are active in a
cell.
Derived mostly from human embryonic kidney 293T (HEK293T) and HeLa
cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target g
cell lines, EdiGene Knockout (KO)
Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target g
Cell Lysates have been optimized through the use of genome editing
technology and validated at the genomic level through PCR and Sanger -
sequencing techniques to ensure the accuracy and knockout of the target gene.
This led to development of newer
technology, called single
cell sequencing (SCS), that has had a major impact in many areas of biology, including cancer research, neurobiology, microbiology, and immunology, and has greatly improved understanding of certain tumor characteristics in cancer.
Using sensitive genetic
sequencing technology, the scientists identified unique RNA splicing variants that distinguish normal, aging stem
cells from abnormal, malignant ones.
Existing
technology, known as next - generation
sequencing (NGS), measures genomes derived from millions of
cells versus the newer method for single -
cell sequencing, called Monovar.
The authors then took advantage of a relatively new
technology known as CRISPR - Cas9 to target those specific DNA
sequences inside a
cell.
Here they photographed organisms from deep - water samples and used
technology to
sequence the genomes of these single -
celled organisms.
UCLA scientists, in collaboration with teams in China, have used the powerful
technology of single -
cell RNA
sequencing to track the genetic development of a human and a mouse embryo at an unprecedented level of accuracy.
A University of Colorado Cancer Center study published in the journal Oncogene used next - generation
sequencing technologies to perform the most detailed DNA - based analysis to date of 25 commonly used bladder cancer
cell lines, allowing researchers to match patient tumors with their closest genetic
cell line match, and demonstrated genetic alterations that may make
cells more or less sensitive to common therapies.
In this proof - of - principle study, the researchers showed, using DNA
sequencing and karyotyping
technologies, that the patient's unique
cell characteristics were maintained in both normal and tumor CR laboratory
cells.
The convergence of several factors explains the trend: cheaper genetic
sequencing technologies, the discovery of new oncogenes (genes that can cause a normal
cell to become cancerous), a new generation of computers and bioinformatics that can analyze vast amounts of data, and a multibillion - dollar effort by researchers inside and outside the pharma industry to develop targeted drugs and companion diagnostics for cancer.
So my field is science and
technology, so my work is circulating tumor
cells where I isolate cancer
cells from blood of patients and isolate their DNA and RNA and try to
sequence them and try to device treatments for cancer treatment and therapy.
To conduct the experiments, the team will use state of the art genome engineering
technology to generate mutant embryonic stem
cells at a large scale, followed by transcriptional profiling via next generation
sequencing.
We seek to «Track and Treat» the cancer
cells present in patients» tissues by using lineage tracing, as well as
sequencing technologies, to determine the molecular mechanisms responsible for the dissemination of cancer
cells to various organs, and resistance to current therapies.
April 2012 - New research: Illuminating embryonic stem
cells Collaboration between two EU funded projects «Heroic» and «EuroSyStem», has provided new insights into embryonic stem
cells The teams used next generation
sequencing technology to examine two key properties of the
cells that influence their identity and behaviour: gene expression and gene regulation.
Using advances in genomic
sequencing, the human microbiome, proteomics, informatics, computing, and
cell therapy
technologies, HLI is building the world's most comprehensive database of human genotypes and phenotypes as a basis for a variety of commercialization opportunities to help solve aging related disease and human biological decline.
These types of investigations have been enabled by key
technologies, including single -
cell assays, high - throughput genomic
sequencing, and improved bioinformatic algorithms.
In a study being published online today in the journal
Cell, researchers in the laboratory of Gladstone Senior Investigator Benoit Bruneau, PhD, employed stem cell technology, next - generation DNA sequencing and computing tools to piece together the instruction manual, or «genomic blueprint» for how a heart becomes a he
Cell, researchers in the laboratory of Gladstone Senior Investigator Benoit Bruneau, PhD, employed stem
cell technology, next - generation DNA sequencing and computing tools to piece together the instruction manual, or «genomic blueprint» for how a heart becomes a he
cell technology, next - generation DNA
sequencing and computing tools to piece together the instruction manual, or «genomic blueprint» for how a heart becomes a heart.
Scientific programs include: human genomic
sequencing and analysis, synthetic genomics and exploration of new vaccines using this
technology, and environmental and single
cell genomics to explore the vast unseen world of microbes living in the human body, the ocean, soil and air.
Cell biology has continued to expand ever since, extending its impact on clinical medicine and pharmacology while drawing on new
technologies in bioengineering, high - resolution imaging, massive data handling, and genomic
sequencing.
Research Focus: I dedicate my time and effort to develop high - throughput
sequencing technologies such as single
cell transcriptomic, ChIP - Seq, ChIA - PET, and many more... The scientific rational is to understand better and with a different angle, the mechanisms of epigenetic regulation of gene expression in (rare) immune
cells, pathologically relevant in many diseases such as asthma, SLE, tuberculosis...
The
technology included a variety of expression systems, including the wheat germ
cell free translation system for eukaryotic gene expression, to take synthetic gene
sequences and translate them into proteins.
Germline Genome Editing Advances in gene - editing
technologies now allow researchers worldwide to modify DNA
sequences inside living
cells quickly, cheaply, precisely, and efficiently.
The recent developments in high throughput single -
cell RNA
sequencing technology (scRNA - seq) have enabled the generation of vast amounts of transcriptomic data at cellular resolution.
To do this I will combine novel
cell isolation techniques with high - throughput sorting and cutting - edge
sequencing technologies.
Using mouse genetics in combination with novel
sequencing technologies our lab is able to identify key
cell types or candidate factors important for specific remodeling and repair processes of the heart.
The organizers of the project, called GP - write (for work in model organisms and plants) or sometimes HGP - write (for work in human
cell lines), envision it as a successor to the Human Genome Project (retroactively termed HGP - read), which 25 years ago promoted rapid advances in DNA
sequencing technology.
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cell culture, transfection, transduction, western blot, PCR, RT - qPCR, Next Generation DNA sequencing, ion torrent, gromacs, ICM, Schrodinger Tools, NCBI software, DIVA, FlowJaw, Flow Cytometry, Cell Sorting, Aria II, Aria III, XCalibur, TaqMan, electrophoresis, Sanger DNA sequencing, FCS Express, cell patterning, ELISA, immunoassay, antibody, HPLC, his - tag, flag - tag, flash - tag, chromatography, stem cells, DNA extraction, CRISPR, cas9, plasmid, lentivirus, CAR - technology, MLV, Chimeric Antigen Receptors, Jurkat, U87, HeLa c
cell culture, transfection, transduction, western blot, PCR, RT - qPCR, Next Generation DNA
sequencing, ion torrent, gromacs, ICM, Schrodinger Tools, NCBI software, DIVA, FlowJaw, Flow Cytometry,
Cell Sorting, Aria II, Aria III, XCalibur, TaqMan, electrophoresis, Sanger DNA sequencing, FCS Express, cell patterning, ELISA, immunoassay, antibody, HPLC, his - tag, flag - tag, flash - tag, chromatography, stem cells, DNA extraction, CRISPR, cas9, plasmid, lentivirus, CAR - technology, MLV, Chimeric Antigen Receptors, Jurkat, U87, HeLa c
Cell Sorting, Aria II, Aria III, XCalibur, TaqMan, electrophoresis, Sanger DNA
sequencing, FCS Express,
cell patterning, ELISA, immunoassay, antibody, HPLC, his - tag, flag - tag, flash - tag, chromatography, stem cells, DNA extraction, CRISPR, cas9, plasmid, lentivirus, CAR - technology, MLV, Chimeric Antigen Receptors, Jurkat, U87, HeLa c
cell patterning, ELISA, immunoassay, antibody, HPLC, his - tag, flag - tag, flash - tag, chromatography, stem
cells, DNA extraction, CRISPR, cas9, plasmid, lentivirus, CAR -
technology, MLV, Chimeric Antigen Receptors, Jurkat, U87, HeLa
cells