Sentences with phrase «cell sequencing with»

The new platform processes hundreds to tens of thousands of cells per day, providing scalable, sensitive, single - cell sequencing with simple yet powerful data analysis.

Not exact matches

Sequencing in one 18 - year - old patient found several oncogenes, and her and her family will continue to work with Human Longevity to monitor the cells.

Each of these cells, like the psyche, is a sequence of actual occasions of experience, each with its own reality and measure of autonomy.

By delivering this version of Cas9 along with the guide RNA strand into single cells, the researchers can target one genetic sequence per cell.

To address this gap in knowledge, Mirabello and Schiffman teamed up with co-senior author Robert Burk of Albert Einstein College of Medicine to sequence the whole genomes of 5,570 HPV16 - infected cell and tissue samples from women around the world and to identify associations between HPV16 genetic variants and the risk of cervical precancer and cancer.

There, some of the added DNA was swapped with the matching target sequences in the cells» genomes.

Scientists at The Institute of Cancer Research, London, and the Wellcome Trust Sanger Institute used DNA sequencing to identify a panel of mutations present across thousands of cancer cells in three patients with leukaemia.

Lu's team will extract immune cells called T cells from the blood of the enrolled patients, and then use CRISPR — Cas9 technology — which pairs a molecular guide able to identify specific genetic sequences on a chromosome with an enzyme that can snip the chromosome at that spot — to knock out a gene in the cells.

He also recommends performing standard bulk - cell sequencing on a portion of the sample, to provide a basis for comparison with the single - cell DNA data.

Cell - free genomic DNA isolated from human cells was cleaved with preassembled, recombinant Cpf1 RNPs and subjected to whole - genome sequencing.

During the formation of eggs and sperm, the cell's chromosomes must pair up and part in an elaborate sequence that results in sex cells with exactly half the number of chromosomes as the parent cell.

To deliver the healthy gene, the team inserted it into an engineered virus called adeno - associated virus 1, or AAV1, together with a promoter — a genetic sequence that turns the gene on only in certain sensory cells of the inner ear known as hair cells.

Coffin and his collaborator, Vinay Pathak, suggested that with each passage, the human cells acquired genetic portions of a murine leukemia virus, which then merged to form a new virus — a hybrid of the parent sequences.

Kelley and his team also used mice with fluorescent markers in different cells of the ear followed by next generation sequencing.

The extra bone that appears in FOP flare - ups progresses through a cartilage stage before replacement with mature bone cells, following a sequence of bone formation seen during normal skeletal development.

Molecules of dsRNA are known to travel between body cells (any cell in the body except germ cells, which make egg or sperm cells) and can silence genes when their sequence matches up with the corresponding section of a cell's DNA.

Combining this mouse model with the reliable RNA sequences allowed an efficient inactivation of genes in primary cells.

They then used next generation sequencing — a state - of - the - art method to rapidly measure gene expression — to sequence and quantify the thousands of genes that are expressed in hair cells, in comparison with other cells in the ear.

«This study demonstrates the potential of combining functional profiling of cells with the characterizations of cancer genomes via next generation sequencing,» said co-senior author Jill P. Mesirov, PhD, professor and associate vice chancellor for computational health sciences at UC San Diego School of Medicine.

Combined with single - cell molecule sequencing, they were able to profile thousands of cells.

Previously developed sequencing techniques require that each cell is isolated before its RNA is labeled with a genetic barcode.

The SC3 tool was then used to analyse single - cell RNA - sequence data from two patients diagnosed with myeloproliferative neoplasm (MPN) blood cancers.

With gene - editing tools such as CRISPR, scientists can now eliminate immune - provoking sugars from the surface of pig cells, introduce human genes that regulate blood coagulation to prevent dangerous clots, and snip out viral sequences that some fear could infect a human host.

Understanding how a particular DNA sequence gives rise to a particular protein provides us with some insight into that protein, but a deeper investigation of how the protein is made, where it is located, and how much of it is present in different cell types is required to enable a true understanding of its function.

Surprisingly, they found that although the patterns of gene expression — as shown by the RNA sequencing — differed between the hepatocellular carcinomas and the liver cancers with biliary phenotype and depended on the histological type, the overall pattern of mutations in the cells was actually similar between the tumors — of either type — that had emerged in patients who had had infections with either hepatitis C or B, and were different in patients without such infections.

In the last couple years, however, hundreds of youngsters with cancer have had the DNA in their noncancerous cells sequenced.

However, Eggan and McCarroll emphasized that now that this phenomenon has been found, inexpensive gene - sequencing tests will allow researchers to identify and remove from the production line cell cultures with concerning mutations that might prove dangerous after transplantation.

The first study to sequence and analyze the entire genome of a HeLa cell line, along with access to its sequence data, has been published Aug. 7 in its final version, by G3: Genes Genomes Genetics, an open - access, scientific journal of the Genetics Society of America.

The new controlled access policy for full genome sequence data from HeLa cells will give the Lacks family the ability to have a role in work being done with the HeLa genome sequences and track any resulting discoveries.

Researchers working with stem cells should follow the example of their colleagues in genetic sequencing and clinical research, setting up global networks for sharing data, materials, and intellectual property, according to a report released today in Washington, D.C..

Now a team at the Wyss Institute of Biologically Inspired Engineering at Harvard University and Harvard Medical School, in collaboration with the Allen Institute for Brain Science, has developed a new method that allows scientists to pinpoint thousands of mRNAs and other types of RNAs at once in intact cells — all while determining the sequence of letters, or bases, that identify them and reveal what they do.

Earlier this year, researchers in Germany published a scientific paper that described the first sequence of the full HeLa genome, comparing the DNA of HeLa cell lines with that of cells from healthy human tissues.

The device has made it possible for researchers to study bacteria and viruses in the field, but its high error - rate and large sequencing gaps have, until now, limited its use on human cells with their billions of nucleotides.

By analogy, the scientists sought to fix RNA in place in the cell, make a tiny ball with many matching DNA replicas of each RNA, then adapt next - gen DNA sequencing so it worked in fixed cells.

Genomics studies like those commonly conducted with HeLa cells play an instrumental role in revealing how variation in genome sequence and function can lead to disease.

These sequences shorten with every cell replication and are considered a strong measure of the aging process in cells.

Without this approach, the results of genomic sequencing would have been swamped with DNA from normal cells, making it difficult to detect cancer - linked DNA errors, Papp says.

Now, Skehel, along with colleagues at Harvard, Yale, and Emory University in Atlanta, Georgia, has used that sequence to build the virus's hemagglutinin (HA)-- a protein that latches onto receptors on the host cell surface — and determine its structure.

With single - cell RNA sequencing, much more gene transcription was detected than before.

«T - cell receptors that are very similar, with slight differences in their sequences, may be recognizing the same antigen.»

Almost all cells in the human body have identical DNA sequences, yet there are 200 - plus cell types with different sizes, shapes, and chemical compositions.

UCLA scientists, in collaboration with teams in China, have used the powerful technology of single - cell RNA sequencing to track the genetic development of a human and a mouse embryo at an unprecedented level of accuracy.

The proteins that bacteria secrete are labeled with unique amino acid sequences, which tell the cells that the proteins are destined for secretion.

A University of Colorado Cancer Center study published in the journal Oncogene used next - generation sequencing technologies to perform the most detailed DNA - based analysis to date of 25 commonly used bladder cancer cell lines, allowing researchers to match patient tumors with their closest genetic cell line match, and demonstrated genetic alterations that may make cells more or less sensitive to common therapies.

The Johns Hopkins team tested ImmunoMap's ability to correlate immune responses on receptor sequencing data from T - cells in the tumors of 34 patients with cancer enrolled in a nationwide clinical trial of the immunotherapy drug nivolumab.

«With our sequencing and transcriptional data, we can figure out which of these cell lines most closely match the human tumors.

«Our combined analysis of cell - free DNA and white blood cell DNA allows for identification of tumor DNA with much higher sensitivity, and deep sequencing also helps us find those rare tumor DNA fragments.»

Future additions to the RM collection may include whole genomes from persons with Hispanic, African and mixed ancestries, as well as a set containing sequenced genes of both malignant tumor and normal cells from the same individual.

Starting in the late 1980s, their labs revealed steps in how the endoplasmic reticulum, the cell's factory for processing secreted and membrane proteins, deals with proteins whose linear sequence of amino acids hasn't folded into a proper 3D shape.

«Understanding how Cas9 is able to locate specific 20 - base - pair target sequences within genomes that are millions to billions of base pairs long may enable improvements to gene targeting and genome editing efforts in bacteria and other types of cells,» says Doudna who holds joint appointments with Berkeley Lab's Physical Biosciences Division and UC Berkeley's Department of Molecular and Cell Biology and Department of Chemistry, and is also an investigator with the Howard Hughes Medical Institute (HHMI).