Sentences with phrase «cell sorting followed»

Berkeley Lab's Len Pennacchio (left) and Axel Visel led the development of a new technique for identifying gene enhancers called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing.

Called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing, this new technique complements existing genomic tools, such as ChIP - seq (chromatin immunoprecipitation followed by sequencing), and offers some additional benefits.

Not exact matches

All of the following antibodies for both analytical and cell sorting cytometry were purchased from BD, except anti-CD27, which was purchased from eBioscience: anti — CD3 - PECy7 or PerCP, anti — CD20 - PECy7 or PerCP, anti — CD38 - PE, anti — CD27 - APC, and anti — CD19 - FITC.

(A) Human tonsillar lymphocytes or (B) sorted Lin - CD127 + cells were pretreated with lethal toxin (1 μg / ml) for 2 — 3 hr followed by IL - 23 (50 ng / ml) stimulation for 18 hrs.

When V+S + cells were recombined with V − S + cells immediately following sorting, they formed normal EBs and the V+S + cells move preferentially to the outside to form the presumptive visceral endoderm.

To avoid the appearance of AID negative clones, cells were cultured in the presence of 0.5 mg / ml of mycophenolic acid for three days following each preparative FACS sort.

Immunofluorescence analysis of the two populations, following overnight adherence to exclude analysis of non-viable cells, revealed that > 97 % of the SSEA3 - positive population expressed detectable SSEA3 / 488 fluorescence and 0 % of the SSEA3 - negative population expressed detectable SSEA3 / 488 fluorescence (Figure 2C), demonstrating that the fluorescence activated cell sorting process can purify viable subpopulations of cells from a heterogeneous somatic population.

(A) Representative images of cell types emerging following culture of sorted subpopulations.

Each Card sort includes the following electrical components: Ammeter, Battery, Bulb, Buzzer, Cell, Switch (closed), Switch (open), Fuse, LDR, LED, Motor, Power supply, Push button switch, Fixed resistor, SPDT switch, Thermistor, Variable resistor, voltmeter, wire.

This bundle includes the following Task Cards: 12 Google Slides Task Cards: • Inserting slides • Changing slide background color • Adding a slide theme / design • Inserting images • Inserting Shapes • Inserting Word Art • Adding slide transition • Adding object animation • Creating Tables • Shading Tables • Downloading files to Microsoft PowerPoint from Google Slides • Uploading Microsoft PowerPoint documents into Google Slides 20 Google Sheets Task Cards: • Inserting columns • Deleting columns • Deleting rows • Inserting rows • Adding currency • Removing currency • Adding decimal places • Removing decimal places • Adding borders • Changing border color • Adding fill color • Sorting data • Merging cells • Unmerging cells • Creating charts • Customizing Pie Charts — A closer look • Moving charts to new sheets • Changing Chart types • Adding New Sheets • Deleting sheets 12 Google Drawings Task Cards: • Creating Tables • Shading tables • Insert Image • Insert Shape • Shape Fill • Line Thickness • Line Style • Line Color • Sending shapes to back • Bringing Shapes to Front • Rotating Shapes • Changing Shapes 12 Google Docs Task Cards: • Creating Tables • Inserting Page Numbers • Inserting images • Formatting Text (Bold, Italic, Underline) • Shading tables • Word count • Line Spacing • Text Alignment • Inserting Bullet Points • Inserting Numbered Lists • Downloading files to Microsoft Word • Uploading Microsoft Word documents into Google Docs

Performs any combination of following duties to assist Pharmacist in Central Fill pharmacy, which includes manual entry and picking, replenishing product for sorting and filling, mixes pharmaceutical preparations, run automated sorting and counting equipment, fills bottles and cells with prescribed tablets and capsules, applies labels to bottles, and verification.